MPSS or massively parallel sanger sequencing is ultra high throughput sequencing technology that sequences millions of mRNA simultaneously and is ideal for whole genome analysis. When applied to expression profiling it reveals almost every transcript in sample and provides accurate expression level.

1. Massively Parallel Signature Sequencing
abbreviated as
MPSS
What is MPSS ?
How it works ?
MPSS vs. Other sequencing
technologies
Application
Dr. Sakshi Vaishnav
(PhD scholar , IVRI)

2. HISTORY
Sydney Brenner
• Co discovered mRNA
• Triplet theory of translation
• Scientific advisory board of Lynx Therapeutics inc.
“ Inventing and implementing technology is much difficult than doing science”

3. • Ultra high throughput
• Analyses level of gene expression
• Counting no. of individual mRNA
INTRODUCTION
Fig.1. GENE EXPRESSION

4. HOW IT WORKS?
Loading cDNA on microbeads (Megaclone technology)
Actual DNA sequencing reaction
Data handling and Calculation of RNA abundance
Statistical analysis of data

5. Lynx Megaclone technology

7. APPLICATION AND ADVANTAGES
 Gene expression
 Variation analysis
 Genome mapping
 Creates digital data rather than analogue data
 Genes expressed at low levels can also be quantified
 No prior requirement of genes to be identified and quantified
 Captures complete transcriptome
 Sequencing - ligation based technique

8. COMPARISION OD MPSS AND OTHER TECHNOLOGIES
MPSS vs. cDNA sequencing
• Both involve preparation of cDNA library
• cDNA sequencing: Purification and sequencing costly and time consuming
MPSS vs. SAGE
• Both generate tag sequence for each mRNA
• Length of tag varies
• MPSS- large dataset for in depth analysis
MPSS vs. microarrays
• MPSS- captures all genes of a sample
• Microarrays – homogenous genes
cross hybridize