2. Dialysis Lab
Take out your lab notebook!
If you have not finished your Cell Observations Lab conclusion,
leave some space for your conclusion and skip to the next page
Title: Dialysis Lab
Purpose: To make observations of a dialysis apparatus in order
to determine how dialysis tubing functions
3. Dialysis Lab – Materials and Method
Materials and Methods:
- Corn Syrup
- Dialysis Tubing
- 500 mL beaker
- 2 rubber bands
- 10mL pipette
- Water
- Clamp and Ring Stand
7. Dialysis Lab - Results
Write down any observations you see
Then write down any questions you have about the
apparatus.
For Example: Why does ______________ happen?
From one question you can come up with a hypothesis
Maybe __________ happens because the pipette has a cotton
in it.
8. Dialysis Lab - Results
Then you can make an experiment around your hypothesis using
an if … then statement
If the cotton ball in the pipette causes the liquid in the pipette
change and we take out the cotton ball then, there will be no
change in the amount of liquid in the pipette.
Here are the materials available for your experiment…
After writing your hypothesis, have an instructor check it and you
can write method for this experiment
Be sure to include
How you will be recording your data
Only have 1 variable – change only 1 thing
A control
9. Gallery Walk
When everyone is done with their method, we will walk around
and annotate each group’s method
Write one thing that you like about the method and one thing
they can do to make it better
Things to look for
Does the method make sense to you? In other words, is it clear?
Do they have a way to record their data qualitatively and quantitatively?
Do they only have one variable?
Make any adjustments necessary and start your new lab.
10. What was happening?
Dialysis is a process
that takes out waste
and extra water
from blood.
It is an artificial
replacement for
kidneys.
11.
12. The dialysis tube is made
of a permeable
membrane that works by
simple diffusion or
passive diffusion.
Passive Diffusion:
Molecules move from an
area of high
concentration to low
concentration until it fills
the space evenly
(equilibrium)
13.
14. Passive Diffusion: Molecules move from an area of
high concentration to low concentration until it fills
the space evenly
Ex: Farts, Purfume, Food coloring in water
15. Cell Membrane
The cell
membrane is
also semi-
permeable and
wants to
balance with the
water from the
environment
outside.
17. Phospholipids: lipids with polar head (hydrophilic) and non-polar
tail (hydrophobic) that make up the cell membrane.
18.
19. But Wait!
If the fatty acid tails on the phospholipid are non-
polar, how do the water molecules go through?
Aquaporins! Proteins in the cell membrane that have a
tunnel for water to pass through.
20. Aquaporins! Proteins in the cell membrane that have a tunnel
for water to pass through using passive transport
21. Types of Transport Proteins
Passive
Transport:
Moving
molecules in and
out of a
membrane
without using
energy. Can only
move from high
to low
concentration
The cell membrane is made up of a phospholipid bilayer where the lipids are really cliqu-ish and like to cluster together.Water on the outside water on the inside