Bial's Test

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Bial's Test

  1. 1. Bial’s Test<br />
  2. 2. Pentoses vs. Hexoses<br />Pentoses<br /> monosaccharide with five carbons in a chain<br />Ex: <br />xylose<br />lyxose<br />arabinose<br />ribose<br />
  3. 3. Bial’s Test<br /> - A test used to detect the presence of pentoses with a test reagent consisting of orcinol, HCl and ferric chloride<br />
  4. 4. Pentoses vs. Hexoses<br />Hexoses<br /> simple sugar contgaining 6 carbon atoms<br />galactose<br />glucose<br />fructose<br />
  5. 5. Procedure<br />1 mLBial’s reagent + 1 mL test solution <br />boil gently until bubbles are observed<br />*Blue green color : +<br />
  6. 6. Results<br />
  7. 7.
  8. 8. Discussion<br />Pentose<br />
  9. 9. Hexoses<br />
  10. 10. Tauber’s Test<br />
  11. 11. Introduction<br />TAUBER’S BENZIDINE TEST<br /> -> more specific for pentoses<br /> -> can detect up to 0.01 mg of the pentose either in its free of combined form<br />
  12. 12. Procedure<br />0.5 mLTauber’s reagent <br /> 5 drops of test solution <br />boil over an open flame for 1-2 minutes <br />*formation of cherry red solution : +<br />
  13. 13. Results<br />
  14. 14. Results<br />
  15. 15. Discussion<br /> It is more specific for pentoses than the bial’s test because it can detect as little as 0.01 mg of a pentose. The presence of a high concentration of hexoses does not interfere with the test. A positive test is indicated by the formation of a cherry red color.<br />
  16. 16. Application<br /> When testing for pentose in urine, the urine (0.1 cc.) and benzidinesolution (0.5 cc.) is brought to vigorous boiling. The mixtureis cooled under tap water and 1 cc. of distilled water is added.In the presence of pentose a pink to red color is shown immediately,whereas if pentose is absent the mixture has a yellowish browncolor.<br />Pentu<br />

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