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A simple, specific, accurate, precise and stability-indicating reverse phase high performance liquid chromatography (RP-HPLC) method is developed for estimation of Dapagliflozin (DGF) in bulk and Pharmaceutical dosage form. The method employed, Hypersil BDS C18 250 mm x 4.6 mm, 5 mm column in isocratic mode with mobile phase of 0.1% Ortho phosphoric acid buffer and acetonitrile 50:50% v/v. The flow rate was 1.0 mL min-1 and effluent was monitored at 245 nm using PDA detector. The injection volume was 10 µl and the total runtime was set as 5min. The retention time for DGF was found to be 2.226min.The method was validated in terms of Linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) etc. in accordance with ICH guidelines. Linear regression analysis data for the calibration plot showed that there was a good linear relationship between response and concentration in the range of 25 - 150 µg/ml respectively. The LOD and LOQ values for HPLC method were found to be 0.04 and 0.121 µg/ml respectively. No chromatographic interference from the tablet excipients was found. The proposed method was successfully used for estimation of Dapagliflozin (DGF) in Bulk and Pharmaceutical dosage form.

Stability indicating RP-HPLC method for estimation of dapagliflozin in bulk a...

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HPLC is a chromatographic technique employed in active compound chemistry and biochemistry to separate a mixture and substances with the goal of identifying, measuring, and purifying the different components of the mixture. Its a much better variety of column and traditional chromatography. The objective of the research work is to develop and validate a simple and accurate reverse phase chromatographic method to estimate amount of drug in dosage form. The developed method successfully can be applied to estimate the amount of Dapagliflozin in tablet dosage form. After oral administration of dapagliflozin, the maximum plasma concentration Concentration max under two hours. High performance liquid chromatographic system was alleviated according to the chromatographic settings. After attaining the steady base line, to verify the system suitability, a single 40 Âµg ml of standard solution proportional to 100 test concentration of dapagliflozin was injected into the HPLC system. The gradient mobile phase flow rate programming assisted in optimising the lengthy run duration and resolution of sample analysis, making the approach more cost effective and quick. Validation of the developed and optimized HPLC method was carried out according to ICH guidelines with respect to parameters such as linearity, specificity, precision and accuracy. Junaid Ahmed | Himanchal Sharma | Shiva Teotia "Method Development and Validation for Estimation of Oral Hypoglycaemic Drug Dapagliflozinina Tablet Dosage form by the Employment of Rp-HPLC" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46395.pdf Paper URL : https://www.ijtsrd.com/pharmacy/analytical-chemistry/46395/method-development-and-validation-for-estimation-of-oral-hypoglycaemic-drug-dapagliflozinina-tablet-dosage-form-by-the-employment-of-rphplc/junaid-ahmed

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Niosomes are nanosized vesicles composed of nonionic surfactants and cholesterol that form when these compounds are dispersed in an aqueous medium. These lipid-based structures are similar to liposomes but differ in their composition, as niosomes use nonionic surfactants instead of phospholipids. The unique characteristic of niosomes lies in their ability to encapsulate both hydrophilic and hydrophobic drugs within their bilayer membrane. This feature makes them promising candidates for drug delivery systems, as they can protect the encapsulated drug from degradation, prolong its release, and enhance its bioavailability. Additionally, niosomes offer advantages such as biocompatibility, stability, and ease of preparation, making them a versatile platform for targeted drug delivery and other biomedical applications.

Niosome An Non-Ionic Surfactant Vesicles.pptx

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Niosomes are a novel drug delivery system that encapsulates the medication in a vesicular system made up of non ionic surfactants. The vesicle is made up of a bilayer of non-ionic surfactants, thus the name niosomes. Niosomes are extremely small and microscopic (on a nanometric scale). Despite having a similar structure to liposomes, they have several advantages over them. Niosomes are biocompatible, nonimmunogenic, and biodegradable in nature and exhibit flexibility in their structured characterization Based on the vesicle size, niosomes can be divided into three groups. Small unilamellar vesicles (SUV, size=0.025-0.05 μm), Multilamellar vesicles (MLV, size=>0.05 μm), and Large unilamellar vesicles (LUV, size=>0.10 μm). In the formulation of niosomes, the selection of surfactants is based on hydrophilic-lipophilic balance (HLB) value. HLB values between 4 and 8 recommended for the facile formation of niosomes and surfactants with an HLB value of more than 8 are required to optimize cholesterol concentration. However, it has been widely observed that HLB value between 4 and 8 is highly recommended for better encapsulation efficiency, of niosomes. For example, long stearyl and short lauryl chain length increase and decrease the entrapment efficiency of niosomes, respectively. Long hydrophilic chains result in increased encapsulation of hydrophilic drugs, and long hydrophobic chains result in improved encapsulation of lipophilic drugs. Long Hydrophilic Chains and Increased Encapsulation of Hydrophilic Drugs: Surfactants with longer hydrophilic chains create larger aqueous compartments within the niosome bilayer. This provides more space for water-soluble drugs to reside, leading to higher encapsulation efficiency. Example: Span 60 (HLB 4.7) has a longer hydrophilic chain compared to Span 20 (HLB 8.6). Studies have shown that using Span 60 in niosomes resulted in significantly higher encapsulation efficiency of the hydrophilic drug gentamicin, compared to formulations using Span 20. Long Hydrophobic Chains and Improved Encapsulation of Lipophilic Drugs: Long hydrophobic chains increase the affinity of the niosome bilayer for lipid-soluble drugs. These drugs can partition and entrap themselves within the bilayer structure, leading to improved encapsulation. Example: Tween 80 (HLB 15) has a longer hydrophobic chain compared to Tween 20 (HLB 16.7). Niosomes prepared with Tween 80 demonstrated superior encapsulation of the lipophilic drug curcumin compared to those made with Tween 20. Pegylation is a process where polyethylene glycol (PEG), a biocompatible and hydrophilic polymer, is attached to the surface of niosomes. This modification offers several advantages for drug delivery: Benefits of Pegylation: Increased Stability: PEG creates a steric barrier, preventing proteins and other molecules in the blood from adhering to the niosome surface. This reduces aggregation and opsonization (recognition by immune cells).

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Non-ionic surfactant vesicles, commonly referred to as niosomes, have garnered significant attention within the pharmaceutical industry due to their remarkable capacity to encapsulate both hydrophilic and hydrophobic drugs. Recent studies have demonstrated the potential of these vesicles to enhance the bioavailability of drugs, making them a promising strategy for delivering various therapeutic agents such as gene materials, protein therapeutics, and chemical pharmaceuticals. This approach offers minimal toxicity and desirable targeting effectiveness. Niosomes are substantially more stable during the preparation and storage procedure than liposomes. The desired pharmacokinetics property can be attained through the optimization of constituents or surface modifications. This novel method of distribution is also facile to establish and expand, while maintaining cost-effective manufacturing expenses. This review article elucidates the fundamentals of niosomes as non-ionic surfactant vesicles, including their structure and components, as well as various formulation methods. Additionally, the article explores the diverse applications of niosomal in the analgesics.

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Introduction: This study explores the use of Response Surface Methodology (RSM), a statistical optimization technique, to optimize the SR properties of prochlorperazine maleate (PCM) matrix tablets. PCM is a phenothiazine derivative used for treating schizophrenia, nausea, and vomiting. Sustained-release formulations offer extended drug delivery, potentially improving patient compliance and reducing side effects. RSM helps identify optimal combinations of critical formulation factors influencing drug release, such as polymer type and concentration, filler type, and drug/polymer ratio. The study likely involves designing experiments based on chosen RSM designs (e.g., Box-Behnken) with varying factor levels. Formulate SR tablets with different factor combinations. Evaluating the drug release profiles of each tablet formulation. Analyzing data using RSM software to build mathematical models relating factors to drug release and identifying optimal factor combinations that maximize desired release characteristics. Objective: The ongoing research purpose to improve the advancement of a sustained release tablet containing Phenothiazine derivative PCM loaded matrix. This is achieved by utilizing DoE as a computational method to statistically validate the formulation.

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Objectives: The ongoing research aims to enhance the development of LNH-loaded nanogel by utilizing DoE as the computational method to statistically validate their formulation. Methodology: In this research Chitosan used as a natural polymer and Poly (Ethylene glycol) [PEG] as a penetration or permeation enhancer. The different nanogel of LNH were synthesized using the Nanoprecipitation and Dispersion method, with variations in the drug-polymer ratio (1/0.03, 1/0.08, 1/0.12). The process parameters were carefully optimizing for enhance the efficiency of the synthesis. To achieve this, optimization studies were conducted using 3² FFD, employing the Design Expert Software Trial version 10.0.7. The total of 13 runs were generated to ensure comprehensive analysis and evaluation of the procedure. The selected independent variables included the concentration of Chitosan (R1) and Carbopol 934 (R2). The dependent variables, on the other hand, were particle size (P1), Polydispersity Index (P2), and % Drug release (P3), chosen in that order. By employing this optimization technique, one can acquire valuable information in a manner that is both efficient and cost-effective. This approach facilitates a deeper comprehension of the relationship between controllable independent variables and the performance and quality of the Nanogels being produced

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Partition coefficient, often denoted as P or P_oct, is a measure of how a solute distributes between two immiscible (unmixable) solvents. It is commonly used in chemistry, biochemistry, and pharmacology to understand the distribution of a compound between different phases, such as between a hydrophobic organic solvent and water. In experimental settings, the partition coefficient is determined by measuring the concentrations of the solute in each phase. The values obtained provide insights into the solute's behavior and can guide decisions in various scientific and industrial processes.

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A pharmaceutical suspension is a heterogeneous system in which finely divided solid particles are dispersed in a liquid medium. Unlike solutions, where solutes are completely dissolved, suspensions involve particles that are only partially soluble or insoluble in the liquid. These suspensions are commonly used in the pharmaceutical industry to deliver medications that may be poorly soluble or unstable in their pure form. The solid particles, often in the form of powders or crystals, are dispersed throughout the liquid phase, creating a stable mixture through the use of suspending agents or stabilizers. These agents prevent the settling of particles, ensuring uniform distribution and ease of redispersion upon shaking before administration. Pharmaceutical suspensions offer advantages in terms of flexibility in dosing and formulation, enabling the delivery of therapeutic agents in various forms such as oral liquids, injectables, or topical preparations, enhancing patient compliance and therapeutic efficacy. The formulation and stability of pharmaceutical suspensions require careful consideration of factors such as particle size, density, and the choice of stabilizers to maintain a consistent and reliable product.

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Suppositories and pessaries are both types of medication delivery systems that are designed to be inserted into body orifices for therapeutic purposes. While they serve similar functions, they are used in different parts of the body. Suppositories: Usage: Suppositories are typically designed for rectal or vaginal administration. Composition: They are solid, bullet-shaped or cone-shaped dosage forms that contain medication in a base that melts or dissolves at body temperature. Rectal Suppositories: Commonly used for medications that need to bypass the digestive system or when a patient cannot take medications orally. They are inserted into the rectum. Vaginal Suppositories: Often used for localized treatment of gynecological conditions, such as yeast infections or hormonal therapy. They are inserted into the vagina. Pessaries: Usage: Pessaries are specifically designed for vaginal administration. Composition: They are solid, oval-shaped or ring-shaped devices made of various materials such as silicone, rubber, or plastic. Indications: Pessaries are mainly used to support the uterus, bladder, or rectum in cases of pelvic organ prolapse. However, they can also be used for the controlled release of medication into the vagina for the treatment of local conditions. Maintenance: Pessaries need to be fitted by a healthcare professional and should be cleaned and reinserted regularly.

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Partition coefficients are a fascinating and important concept in many fields, from chemistry and environmental science to medicine and pharmacology. They tell us about how a substance will distribute itself between two immiscible phases, like how a drug might move between your blood and tissues, or how a pollutant might spread through soil and water. A partition coefficient, denoted as P or log P, describes the ratio of the concentration of a compound in one phase (usually organic) to its concentration in another phase (often water) at equilibrium. Higher values of P indicate a greater preference for the organic phase, meaning the compound is more lipophilic (fat-loving). Lower values of P suggest a higher affinity for the aqueous phase, implying the compound is more hydrophilic (water-loving).

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This systematic review aims to provide a comprehensive overview of the current status of mucosal drug delivery systems (MDDS) and explore their future prospects in drug delivery. MDDS have gained significant attention in recent years due to their potential to enhance drug absorption, improve therapeutic efficacy, and minimize systemic side effects. This review critically evaluates the existing literature on MDDS, including various mucosal routes such as oral, nasal, ocular, pulmonary, and vaginal delivery. Additionally, it discusses the challenges associated with MDDS, such as formulation development, stability, and regulatory considerations. Furthermore, this review highlights emerging technologies and innovative strategies that hold promise for the future of MDDS. Overall, this systematic review provides valuable insights into the current landscape of MDDS and offers recommendations for future research and development in this field.

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Three-dimensional (3-D) printing is elevating various growth in production viewpoint both at nanoscale and macro-scales. 3-D printing is being scouted for numerous bio-pharmaceutical administration and creation of nano-medicines employing supplementary production methods and shows assurance in capability in satisfying the demands for a patient-based customized approach. The previous outcome features the accessibility of novel natural bio-materials and finely designed polymeric substances, which can be created as unique 3-D printed nano-materials for numerous bio-pharmaceutical administrations as nano-medicines. Nano-medicine is described as the utilization of nanoscience in fabricating nano-materials for various pharmaceutical utilization, comprising identification, cure, scan, stopping, and management of diseases. Nano-medicine has also displayed a huge effect in the creation and evolve an accurate drug. In contrary the "one-size-fits-all" benchmark for the traditional drug is a personalized, structured, or accurate drug considering the variation in numerous characteristics, comprising genetics and pharmacokinetics of various victims, which have exhibited better outcomes over traditional cures. This article highlights the approaches advancements in the design and development of customized-made nano-medicine employing 3-D printing science.

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Definition: TQM has been defined as an integrated organization effort designed to improve quality at every level. “ The process to produce a perfect product by a series of measures require an organized effort by the entire company to prevent or eliminate errors at every stage in production is called Total Quality Management (TQM).” The Aim of TQM is “Prevention of defect rather than detection on defect.” TQM is very important for pharmaceutical industries to produce the better product and ensure the maximum safety of health care system and also protect waste of money for both government and individual customer.

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The word pharmacy is derived from the Greek word “Pharmakon”, meaning medicine or drug. In other term, “Pharmacy may defined as the art and science of preparing (manufacturing) and dispensing of drugs prepared by the natural and synthetic sources and using for the treatment as well as prevention of diseases”. In general sense, it is the place where medicine or drugs are sold. Pharmacy is a health profession that links health science with chemical science and aims to ensure the safe and effective use of pharmaceutical drugs. It includes the collection, identification, synthesis, purification, isolation and quality control of medical substance or pharmaceutical products.

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