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Reagents

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Reagents

  1. 1.  BY:  V.SRINANDAN  M.PHARMACY 1ST YEAR  DEPT. OF PHARM ANALYSIS
  2. 2.  It is a substance or mixture for use in chemical analysis or in chemical reactions.  It is used for both qualitative and quantitative analysis.
  3. 3. REAGENTS Aldehydes Ketones& Quinones Amines Redox reagents • PDAB • PDAC • Ninhydrin • Folin-Ciocalteau • 2,6-dichloroquinone Chloride • 1,2-napthaquinone – 4-Sulfonate • 3-mehyl-2 benzthiazole Hydrazone • 2,3,5-Triphenyl tetrazolium • 2,4Dinitropheny l Hydrazine • Bratton- Marshall Reagent • 2,3,5- Triphenyl tetrazolium • 3,5- Dinitrosalicy lic acid Amines Aldehydes& Ketones CLASSIFICATION
  4. 4. P-DIMETHYL AMINO BENZALDEHYDE[PDAB] C PRINCIPLE:
  5. 5. APPLICATIONS
  6. 6. P-DIMETHYL AMINO CINNAMALDEHYDE [PDAC] PRINCIPLE: + PDAC
  7. 7.  To detect indole production in cell.  Rapid identification of bacteria containing tryptophan enzyme system.  Analysis of flavonoids.  Identification of micro organism in urine medium  Study of narcotic drugs APPLICATIONS
  8. 8. NINHYDRIN: Ninhydrin Amino Acid Reduced Ninhydrin + -2H2O Purple colour complex PRINCIPLE:
  9. 9. APPLICATIONS  Quantitative estimation of antibiotics Bacitracin Cycloserine Polymixin B  Estimation of drugs like Folic acid Methyldopa Cephalenin Salbutamol  For protein analysis  In bone analysis  In finger print detection
  10. 10. 2,6-Dichloroquinone-4-chloroimide + Pyridoxine Coloured Product2,6dichloroquinone mono imine
  11. 11.  Colorimetric determination of pyridoxine( indo phenol ester)  Chloramphenicol can be estimated at 555nm it produce purple color  Procaine HCl at 550nm, it produce pink color.  Folic acid can measure at 550nm.  Sulphonamides can also estimated. APPLICATIONS
  12. 12. 3- METHYL BENZOTHIAZOLINONE HYDRAZONE (MBTH) PRINCIPLE:
  13. 13. It is used in the determination of the following  Phenols  Formaldehyde  Aldehydes  Poly hydroxyl compounds  Primaquine- pink(510nm)  Adrenergic Drugs like Salbutamol, Pholedine APPLICATIONS
  14. 14. In diazonium salt electrophilic reaction takes place PRINCIPLE: Sulphonamide diazotization Diazo compound + BMR coupling Pink color Diazo compound BMR coloured complex BRATTON MARSHALL REAGENT
  15. 15.  Estimation of sulphonamides Eg: sulphamethaxazole- yellow color at 445nm sulphamethazine- pink color  In clinical analysis; solution deproteised with alcohol chloramphenicol- purple color at 555nm procaine - pink color at 550nm  Application in quantitative inorganic analysis of nitrates, nitrite and sulphonamide in blood. APPLICATIONS Chloramphenicol Procaine
  16. 16. 2,3,5-TRIPHENYL TETRAZOLIUM CHLORIDE Redox indicator. PRINCIPLE: Steroids(alpha-keto grp) succinate dehydrogenase TTC (white colour) 1,3,5-triphenyl formazan (red colour) {Water soluble } {water insoluble} 𝑅𝐻2 Dehydrogenase R + 2𝐻+ +2𝑒− (Substrate) ( Oxidised substrate) Tri-phenyl formazan is formed and extinction in measured in blank reagent
  17. 17.  For reducing sugars and also distinguish between alpha-ketone and simple aldehyde.  For reducing plant and animal tissues.  Molybdenum in the plant extract at 250nm.  Autopsy_ pathology of Heart tissue. APPLICATIONS

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