14. RULES WHILE COUNTING CELLS IN
EACH SQUARE
• Count only the cells present inside each
square
• Cells on the line – count only the cells that
are there on the TOP and LEFT border
• Omit those on the RIGHT border & lower
border
• This will avoid counting the same cell again
18. BLOOD COLLECTION
Capillary/peripheral blood collection
Things required: Disposable lancet, spirit, cotton
• Select the finger tip
• Clean the tip with spirit
• Allow it to dry
• Make a prick with the lancet
• Wipe the first drop – blood diluted with tissue
fluid
• Allow the blood to flow freely- do not squeeze
or milk the finger to get blood
20. METHOD OF DILUTION
• Take blood up to the 0.5 mark without any
air bubble
• Take the dilution fluid upto the 11 or 101
mark
• Keep the pipette between the palms and mix
the fluid
• The fluid in the bulb gets mixed completely
21. DILUTION FACTOR
• Blood taken till 0.5 mark
• Dilution fluid taken till 101 mark
• Fluid in the bulb is blood + dilution fluid
• Fluid in the stem is cell free
• Discard the stem fluid – roughly 2-3 drops
• So 0.5 blood is diluted in 100 dilution fluid or
• 1 blood is diluted in 200 dilution fluid
• Dilution = 1: 200