APPLICATIO OF RDNA TECHNOLOGY SHORTLY

1. RECOMBINANT DNA TECHNOLOGY 1

2. RECOMBINANT DNA TECHNOLOGY
2
APPLICATIONS OF RECOMBINANT D
NA TECHNOLOGY
BABAR KHAN
UOS,SWAT
MICROBIOLOGY

3. ☆ RECOMBINANT DNA TECHNOLOGY
 Recombinant DNA technology, joining
together of DNA molecules from two
different species that are inserted into
a host organism to produce new genetic
combinations that are of value to science,
medicine, agriculture, and industry.
RECOMBINANT DNA TECHNOLOGY 3

4. ☆ APPLICATIONS
 Application in medicine
 Application in forensic science
 Application in agriculture
 Application in food industry
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5. ☆ APPLICATION IN MEDICINE
 Recombinant DNA technology had made it possible
to treat different disease by inserting new genes in
place of mutated gene in human body.
 Brought many revolutionary changes in the field of
medicine and introduce such method of treating
diseases and delivering the drug.
 Recombinant DNA technology in medicine also used
in a series of procedures that are used to join
together DNA segments.
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6. • A recombinant DNA molecule is constructed from
of two are more different DNA molecules
• under certain condition recombinant
DNA molecule can enter
A cell and replicate
their either on its own or
after it has been
integrated into
Chromosome
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7. 1. INSULIN
 Insulin is a hormone
 made of protein
 secreted in the pancreas by some cells called as islet of
Langerhans.
 This hormone is responsible for controlling the glucose
level in humans.
 If a person has decreased amount of insulin in his body,
he will suffer from a disease called diabetes.
 Recombinant DNA technology has allowed the scientists
to develop human insulin by using bacteria as a host cell
.
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8. 2. vaccines
 vaccines is a biological substances that is prepared
from the suspension of weak or dead pathogenic cells
 it is injected in the body to enhance the production of
antibodies against a particular antigen
 recombinant DNA technology has made it easier for
scientists to develop vaccine by cloning a gene used for
protective antigen protein
 viral vaccine are mostly develop in this technique
 Example : HBV
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9. 3.INTERFERON
 Interferon is a glycoprotein which has the ability to block
the multiplication or division of viruses in the cell or in the
nearby cells
 Interferon can be used to treat cancer like hairy cell
leukemia
 recombinant DNA technology produced this protein using
a bacteria called E.coli
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10. 4.ANTIBIOTICS
 Chemical substances
 used against bacterial infections
 produced by microorganisms as well as in the laboratory
 having the ability to destroy microbes that cause harmful
infections in the body
 Alexander Fleming discovered penicillin in 1928 using
recombinant DNA technology
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11. ☆ APPLICATION IN FORENSIC SCIENCE
 It helps the human being in investigation system to fight
against crime and sort out civil disputes
 When a person commits a crime for instance murder, the
y will leave behind small amount of blood, saliva semen
hair , nails or anything else at the crime scene or in a vict
im
 in such circumstances DNA fingerprinting could help in i
dentifying them
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12. ☆ APPLICATION IN AGRICULTURE
 Recombinant DNA changes the natural genetic makeup
and the characteristics of an organism by inserting DNA
of another organism
 also known as genetic engineering
 Recombinant DNA technology is widely used in agricultu
re to create genetically modified organisms that produce
genetically modified crops
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13. APPLICATION IN AGRICULTURE
 Recombinant DNA technology increased productivity of c
rops
 Enhance crop protection
 Improvement in food processing
 Improved nutritional value
 Better flavor
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14. ☆APPLICATION IN FOOD INDUSTRY
• The recombinant dna technology plays an important role
in the production of food
• The food processing enzymes from recombinant
microorganisms are used in the production of food such as
cheese
• Enzymes which are traditionally isolated from culturable
microorganism,plant and mammalian tissues are offten not
well adapted to the condition used in modren food
production methods
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15. • Thats why we need recombinant dna technology
Steps in manufacture novel enzymes suitable for
food processing conditions
HOW..??
• we can do it by screening
Microorganisms sampled
from diverse environments
• Do modification of known
enzymes using modern of
protein engineering
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16. Example
• Chymosin
• It also known as rennin single polypeptide chain of 323
amino acids
• it possesses two different types of chymosin which are A
and B
• The main coagulating enzyme found in rennin which is
used extensively in cheese production
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17. ☆Steps
• First ,Gene for chymosin are isolated from cow DNA
• second, the plasmid DNA is cut restriction enzymes
• 3rd,then combine chymosin DNA and plasmid DNA with
aid of ligase
• put the recombinant DNA
Into the bacteria through
the process of
transformation
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18. • Chymosin protein is then express into the bacterial
Cells
• after chymosin protein are isolate and use for making
Cheese.
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19. ssssss
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