Sterilization by radiation ionized Xray Gamma ray non ionized ultraviolet rays Ionized radiation ionize water to highly reactive free radicals which can break strands of DNA non ionized radiation ultraviolet ray induce pyrimidine dimers in the nucleic acid mutation in in critical genes cause bacterial cell death used to sterilize heat labile solution decontaminate hospital operating room food processing areas Gamma rays produced from radioactive isotope especially cobalt60 for disposable plastic Infrared sun light infra red lamp sterilize by heating Mechanical sterilization by filtration sartorius filter syringe filter removal of bacteria from media by mechanical method used for sterilization of serum culture media bacteriological media membrane filter with 0.45---0.2 millimicron disc

1. Sterilization by radiation
ionized
Xray
Gamma ray
non ionized
ultraviolet rays

2. Ionized radiation
ionize water to highly reactive free radicals
which can break strands of DNA
non ionized radiation
ultraviolet ray induce pyrimidine dimers in the
nucleic acid
mutation in in critical genes cause bacterial cell
death used to sterilize heat labile solution
decontaminate hospital operating room
food processing areas

3. Gamma rays
produced from radioactive isotope especially
cobalt60 for disposable plastic

4. Infrared sun light infra red lamp
sterilize by heating

5. Mechanical sterilization
by filtration
sartorius filter
syringe filter
removal of bacteria from media by mechanical method
used for sterilization of serum
culture media
bacteriological media
membrane filter with 0.45---0.2 millimicron disc