The Center of Excellence in Cancer Research (CECR) was established in June 2013 at Tanta University Educational Hospital in Egypt. It is directed by Dr. Mohamed L. Salem and focuses on correlating immunologic and clinical data from genomic, transcriptomic, and proteomic profiling of circulating and primary tumor cells. The CECR has core facilities for flow cytometry, cell sorting, and genomic and proteomic analysis to support its research.

1. Center of Excellence in
Cancer Research
(CECR)
Director, Dr. Mohamed L. Salem, PhD
Prof. of Immunology
Tanta University, Egypt

2. Location
Location
Tanta University Educational Hospital

3. Established Date
Established Date
 June 2013 through a project funded by Science and
Technology development Fund (STDF)
 PI: Prof. Mohamed Labib Salem
 Affiliation: Tanta University

4. Dr. Mohamed L.Salem
Prof. of immunology
Dr. Said Hammad
Prof. of clinical Pathology
Dr. Mohamed Ramadan
Prof. of Pediatric
Dr. Mohamed Attia
Assis. Prof. of Clinical Pathology
Dr. Abdel-Aziz Zidan
PhD of Immunology
Ms. Mona Fouad Zidan
Researcher Assistant
Research Team

5. Research Team

6. Organogram

7. CECR Main Project
Correlating immunologic and clinical performance
between genomic, transcriptomic and proteomic
profiling of circulating tumor cells versus primary
tumor cells

8. CECR Core Facility

9. Flow Cytometry Unit
Nomination:
The BD FACSCanto derives its name from the musical term “bel canto” meaning
operatic singing that focuses on ease and purity of tone with an agile and precise
vocal technique.
• BD FACSCanto II Cytometer

10. SpSepceifciicfiacatitoionnss ooff t hthe ein isntrsutmruenmt ent
• The BD FACSCanto system has a capacity for three lasers, eight-parameter
detection while capturing particle sizes from 0.5 to 50 μm in diameter.
• Digital electronics for processing up to 10,000 events per second, and a novel
sample injection tube supporting
Multiparameter detection helps in:
• Advanced cell subset analysis.
• Multiparameter functional studies.
• Soluble protein detection.
• Quantification studies for cell-surface antigens and soluble proteins.
• Decreases the total sample volume necessary for a specific project as more
parameters.

11. SpReesceiafriccha t&io Cnlisn iocfa lt ahpep liincasttironusm ofe tnhte instrument
• Immunophenotyping by flow cytometry is an important tool in the diagnosis and
staging of patients with a haematological neoplasm as well as solid tumors.
• Some of the flow cytometry applications in diagnosis of different diseases are listed
below:
Detection of
minimal
residual disease
Stem cell
enumeration
Analysis of
Leukemias and
Lymphomas
BD FACSCanto Applications
Reticulocyte
analysis
Detection of
Immunodeficiency
diseases
Detection of HIV
infection
Analysis of different
types of solid
tumors.
Detection of
autoantibodies

12. Cell Sorting Unit
 The IsoFlux system enriches rare cells from biological samples and
prepare them for further analysis , It gives immediate access to high-quality
, viable cells in a high density format , ready for use in the
application of your choice
 Benefits:
• Access a wide range of samples.
• Use your own capture criteria.
• Capture intact, pure cells.
• Minimize Sample Diluation
• Save Time
• Save Space
• Utilize validated CTC analysis workflows.

13. Cell Sorting Unit
Enumeration Immunofluorescence
IsoFlux Applications
Fluorescence in situ
hybridization
Next generation
sequencing
Mutation detection Gene expression

14. Genomic & Proteomic Unit
 Microarray
A high throughput technology that allows detection of thousands
of genes simultaneously.
Features:
• High samples throughput.
• Laser excitation at 532nm, dual- laser excitation .
• Scanning pixel resolution 2μm
• Scan sequence: simultaneous scan and independent scan.
• Scan/read time: approximately 5 samples/hour.

15. Genomic & Proteomic Unit
Microarry Applications
Transiptome
mRNA Expression
miRNA Expression
Splice Variance
Genome Structure
DNA Copy number
Genome Variation
Single Nucleotide Polymorphism
Copy number Variations
Proteome
Protein-Protein Interactions
DNA – Protein inreraction
Epigenetics
DNA Methylation
Chromatin Structure

16. Genomic & Proteomic Unit
• LumineX200

17. Genomic & Proteomic Unit
 Flexible Multiplexing Technology
• Open Platform for Genomic & Proteomic assays
• Life Science Research & Diagnostic assays available.
• Broad menu of commercial assays and laboratory developed tests
• Multiplex up to 500 tests in one well of microliter plate.
• Decrease sample volume requirements.
• Reduce assay reagent volume, expense & labor.
• Generate more information on interrelationships between
analytes within a single sample.

18. Genomic & Proteomic Unit
Clinical Diagnostics Clinical Research Life Science Research
Luminex Applications
Biosurveillance Assay Development Tools

19. CECR Collaborators CECR Collaborators

20. CECR Conferences
Monday 24 February 2014
The 1st Annual Conference of
The Center of Excellence in Cancer Research
Next Generation Cancer Research & Diagnosis
In Collaboration with
Research Centers of Excellence
(STDF- Affiliated Centers)