Microscopy and Imaging

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Microscopy and Imaging

  1. 1. Microscopy & Imaging Shared Resource(MISR) Michael Johnson, Ph.D. MISR Director Jan Blancato, Ph.D. Cytogenetics and immunofluorescence Peter Johnson, B.S. Manager Susette C. Mueller, Ph.D. Consultant
  2. 2. MISR Provides: <ul><li>Routine and advanced cellular imaging and image analysis for tumor and cell biology studies. </li></ul><ul><li>Guidance for tumor biology studies using quantitative imaging: subcellular changes in protein distribution. </li></ul><ul><li>Access to advanced imaging instrumentation hardware and image analysis software including Metamorph and Volocity . </li></ul><ul><li>Cytogenetics resources and services such as DNA FISH. </li></ul>
  3. 3. MISR Services: <ul><li>TIRF </li></ul><ul><ul><li>Fluorescence, single molecule </li></ul></ul><ul><ul><li>150 nm adjacent to coverslip </li></ul></ul><ul><ul><li>Multicolor fluorescence at the plasma membrane (only!) </li></ul></ul><ul><ul><li>Applications: </li></ul></ul><ul><ul><ul><li>Membrane receptors </li></ul></ul></ul><ul><ul><ul><li>Exocytosis, endocytosis </li></ul></ul></ul><ul><ul><ul><li>Cell adhesions (TIRF/IRM) </li></ul></ul></ul>TIRF ( Total Internal Reflection Fluorescence microscopy)
  4. 4. MISR Services: <ul><li>Confocal/ Multiphoton </li></ul><ul><ul><li>Single cell or deep tissue (up to 500 mm maximum) </li></ul></ul><ul><ul><li>Live cell imaging </li></ul></ul><ul><ul><li>Second harmonic detection of collagen fibers </li></ul></ul><ul><ul><li>FRET/FLIM for protein-protein study </li></ul></ul>Fluoview FV300 Zeiss LSM510/META/NLO
  5. 5. MISR Services: <ul><li>Spinning disk confocal (live) imaging </li></ul><ul><ul><li>Fast, 3D confocal imaging of cells </li></ul></ul><ul><ul><li>Volocity 2D and 3D image analysis </li></ul></ul><ul><ul><li>Multiwell, automated image collection </li></ul></ul><ul><li>Routine fluorescence </li></ul>
  6. 6. MISR Training: <ul><li>Training of users through lectures, workshops, and individual instruction. </li></ul><ul><li>Some of the equipment can be used by trained users. Technical assistance can always be requested. </li></ul>
  7. 7. MISR Contacts <ul><li>Mike Johnson, PhD </li></ul><ul><li>Core Director as of 7/1/10 </li></ul><ul><ul><li>x7-0217 (W414 Res. Bldg.) </li></ul></ul><ul><li>Jan Blancato, PhD </li></ul><ul><li>Cytogenetics </li></ul><ul><ul><li>x7-0217 (W414 Res. Bldg.) </li></ul></ul><ul><li>Peter Johnson, B.S. </li></ul><ul><li>Manager </li></ul><ul><ul><li>x7-1916 (W407/W408 Res. Bldg.) </li></ul></ul><ul><ul><li>[email_address] </li></ul></ul><ul><li>ZeissLSM and TIRF (E308 Res.Bldg) </li></ul><ul><ul><li>x7-3168 (E308 Res. Bldg.) </li></ul></ul>
  8. 8. Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01    In your publications, remember to mention support provided from the GHUCCTS.

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