This document studies the effects of sporamin, a protein from sweet potatoes, on human gut cancer cell lines. It finds that sporamin exerts significant anti-proliferative and anti-metastatic effects by inducing apoptosis in human pancreatic, esophageal, and colorectal cancer cell lines. Specifically, sporamin treatment was found to suppress tumor growth through influencing expression of Bcl-2 family proteins and inhibiting the NF-κB pathway. Figures 1-3 show results of experiments demonstrating sporamin's dose-dependent inhibition of cancer cell proliferation and induction of apoptosis.
Connective Tissue II - Dr Muhammad Ali Rabbani - Medicose Academics
CONFERENCE POSTER PHARMACOGNOSY.pdf
1. Finding phytoproteins that suppress tumour growth on
gut micro environment
Sporamin from Ipomoea batatas
(sweet potato) provide the first clue
RESULTS
METHODOLOGY
AIM
FINDINGS
Fig.1. Effects of sporamin
treatment on the expressionof
Bcl-2, Bcl-XL, and Bax in
human pancreatic cancer cell
lines, PANC-1 and BxPC-3
(western blot)
BACKGROUND
Fig. 2. Effects of sporamin
treatment on the expression
levels of Bcl-2, Bcl-XL and Bax in
esophageal squamous cell
carcinoma cell lines , EC9706 and
EC109 (western blot)
Fig. 3. Effect of sweet potatoprotein
on human colorectal cancer SW480
cells.
A: Effects of various concentrations
of sweet potatoprotein (SPP) on
SW480 cell proliferation.Observed
dose-dependent inhibition.
B: Effect of various treatment times
with 40 μmol/L SPP on SW480 cell
proliferation.Time-dependent
inhibitionobserved.
C: Hoechst 33258 nuclear staining.
SW480 cells were incubated in the
absence or presence of various
concentrationsof SPP for 48 h,
stained with Hoechst 33258 dye.
Sporamin exerts
significant anti-
proliferative and anti-
metastatic effects by
inducing apoptosis of
human gut cancer cell
lines.
ETHNOBOTANY
The sweet potato (Ipomoea
batatas) is a dicotyledonous
plant, Family-Convolvulaceae.
✓anti-cancer
✓Anti-diabetic
✓Anti-inflammatory
To study whether sporamin, a
storage protein from sweet potato,
suppresses the growth of tumour
cells of human GI tract in vitro.
Electronic databases
searching:
PubMed, Google scholar, and
ScienceDirect
Reference:
1. Qian C, Chen X, Qi Y, et al. Sporamin induces apoptosis and inhibits NF-κB activation in human pancreatic cancer cells. Tumor Biology. 2017;39(7).
doi:10.1177/1010428317706917
2. Qian, C., Qi, Y., Chen, X., Zeng, J., & Yao, J. (2017). Sporamin suppresses growth of human esophageal squamous cell carcinoma cells by inhibition of
NF-κB via an AKT-independent pathway. Molecular Medicine Reports, 16, 9620-9626. https://doi.org/10.3892/mmr.2017.7772
3. Li PG, Mu TH, Deng L. Anticancer effects of sweet potato protein on human colorectal cancer cells. World J Gastroenterol 2013; 19(21): 3300-3308
[PMID: 23745032 DOI: 10.3748/wjg.v19.i21.3300]
Induction of cell apoptosis in
human cancer cell lines in vitro
![Finding phytoproteins that suppress tumour growth on
gut micro environment
Sporamin from Ipomoea batatas
(sweet potato) provide the first clue
RESULTS
METHODOLOGY
AIM
FINDINGS
Fig.1. Effects of sporamin
treatment on the expressionof
Bcl-2, Bcl-XL, and Bax in
human pancreatic cancer cell
lines, PANC-1 and BxPC-3
(western blot)
BACKGROUND
Fig. 2. Effects of sporamin
treatment on the expression
levels of Bcl-2, Bcl-XL and Bax in
esophageal squamous cell
carcinoma cell lines , EC9706 and
EC109 (western blot)
Fig. 3. Effect of sweet potatoprotein
on human colorectal cancer SW480
cells.
A: Effects of various concentrations
of sweet potatoprotein (SPP) on
SW480 cell proliferation.Observed
dose-dependent inhibition.
B: Effect of various treatment times
with 40 μmol/L SPP on SW480 cell
proliferation.Time-dependent
inhibitionobserved.
C: Hoechst 33258 nuclear staining.
SW480 cells were incubated in the
absence or presence of various
concentrationsof SPP for 48 h,
stained with Hoechst 33258 dye.
Sporamin exerts
significant anti-
proliferative and anti-
metastatic effects by
inducing apoptosis of
human gut cancer cell
lines.
ETHNOBOTANY
The sweet potato (Ipomoea
batatas) is a dicotyledonous
plant, Family-Convolvulaceae.
✓anti-cancer
✓Anti-diabetic
✓Anti-inflammatory
To study whether sporamin, a
storage protein from sweet potato,
suppresses the growth of tumour
cells of human GI tract in vitro.
Electronic databases
searching:
PubMed, Google scholar, and
ScienceDirect
Reference:
1. Qian C, Chen X, Qi Y, et al. Sporamin induces apoptosis and inhibits NF-κB activation in human pancreatic cancer cells. Tumor Biology. 2017;39(7).
doi:10.1177/1010428317706917
2. Qian, C., Qi, Y., Chen, X., Zeng, J., & Yao, J. (2017). Sporamin suppresses growth of human esophageal squamous cell carcinoma cells by inhibition of
NF-κB via an AKT-independent pathway. Molecular Medicine Reports, 16, 9620-9626. https://doi.org/10.3892/mmr.2017.7772
3. Li PG, Mu TH, Deng L. Anticancer effects of sweet potato protein on human colorectal cancer cells. World J Gastroenterol 2013; 19(21): 3300-3308
[PMID: 23745032 DOI: 10.3748/wjg.v19.i21.3300]
Induction of cell apoptosis in
human cancer cell lines in vitro](https://image.slidesharecdn.com/meenaloshinieposter-230220131738-29aeec8c/85/CONFERENCE-POSTER-PHARMACOGNOSY-pdf-1-320.jpg)
