1. Jack Ryan Reifert
Home Address: 2528 Orella St. Unit-B • Santa Barbara, CA 93109 • 858-922-5923 • reifert@lifesci.ucsb.edu
Work Address: Neuroscience Research Institute • BLDG. 571, RM. 6131 • Santa Barbara, CA 93106-5060
Education:
Ph.D. Molecular, Cellular, and Developmental Biology (MCDB) / Neuroscience Research Institute 2005-2011
University of California, Santa Barbara (UCSB)
B.S. Pharmacological Chemistry 1996-2000
University of California, San Diego (UCSD)
Additional Education:
• Technology Management Program at UCSB: Business education courses pertaining to the health care industry. 2009-2011
• UCSD Extension courses: Introduction to Pharmacology, Mechanisms of Disorders I and II, Neuropharmacology 2002-2005
Industrial Research Experience:
Salmedix INC. La Jolla, CA / Research Associate 2002-2005
Bendamustine (Treanda), won FDA approval for treatment of Chronic Lymphocytic Leukemia (CLL)
and Non-Hodgkin’s Lymphoma (NHL), 3 years after being acquired by Cephalon pharmaceuticals.
• Working with the biology research group, our work directly influenced Bendamustine clinical trails for B-cell NHL
and provided evidence for a mechanism of action divergent from other chemotherapies. (See publication for reference.)
BD Pharmingen. La Jolla, CA / Quality Control Associate I 2001-2002
• Coordinated team meetings to efficiently approach “problem” products, resulting in release of multiple reagents
from backorder and updated data sheets with new information specific to each product.
Academic Research Experience:
Chemical Engineering (UCSB), Santa Barbara, CA / Postdoctoral Fellow (CIRM) 2011-Present
• Topic: Using bacterial peptide display for the detection and inhibition of proteases involved in ovarian cancer
metastasis with an emphasis on targeting cancer stem cells.
Neuroscience Research Institute (UCSB), Santa Barbara, CA / Graduate Research Assistant 2005-2011
• Topic: Mechanisms of amyloid beta induced neurodegeneration - involvement of microtubule associated protein tau.
Sam and Rose Stein Institute for Research on Aging (UCSD), La Jolla, CA / Research Associate 1999-2001
• Was an author on two publications for investigating the involvement of wnt / β-catenin signaling in the promotion
of rheumatoid arthritis using primary synoviocytes isolated from patients.
Selected Publications:
Reifert J, Hartung-Cranston D, Feinstein S.C. “Tau fragmentation without marked changes in tau phosphorylation precedes
amyloid beta mediated cell death in cultured hippocampal neurons.” J Biol Chem. 286(23): 20797-811. Jun. 2011
Leoni LM, Bailey B, Reifert J, Bendall HH, Zeller RW, Corbeil J, Elliott G, Niemeyer CC. “Bendamustine (Treanda) displays a
distinct pattern of cytotoxicity and unique mechanistic features compared with other alkylating agents.” Clin Cancer Res. 14(1): 309
17. Jan. 2008
Sen M, Reifert J, Lauterbach K, Wolf V, Rubin JS, Corr M, Carson DA. “Regulation of fibronectin and metalloproteinase expression
by Wnt signaling in rheumatoid arthritis synoviocytes.” Arthritis Rheum. 46(11): 2867-77. Nov. 2002
Selected Research Skills:
Dissection and culture of primary neurons RNA, DNA, protein isolation Site-directed mutagenesis
Glial cell isolation Molecular cloning/subcloning Flow cytometry
Tissue embedding and sectioning PCR, qPCR, primer design Live and fixed-cell imaging
Mammalian tissue culture HPLC Protein purification Peptide display library design
Immunoprecipitation Transformation / Transfection Size exclusion chromatography
In-vivo drug delivery (oral gavage, IV) Western blotting / 2D Westerns Virus production / infection
Multiple microscopy systems including fluorescent, confocal, and DSU. Cytotoxicity assays