1. DAVID M. LACASELLA
39318 SALINAS DRIVE, MURRIETA, CA 92563, {951}600-7627, DLACASELLA@AOL.COM
PROFILE
Highly professional Lab Assistant with over two years’ experience. Capable of doing bench work or in-
vitro research studies. Possess a Biotech Certificate fromCal State San Marcos. Experience with a wide
range of lab equipment and able to follow a variety of SOP’s. Exceptionally detail oriented, meticulous
and observant.
PROFFESIONAL EXPERIENCE
10/26/2013 TO PRESENT Nitto Denko Oceanside, CA
LAB ASSISTANT RESEARCH AND DEVELOPMENT
 Work under the direction of Lead Scientist doing research for photoreactive materials
 Prepare adhesive solution with ETOH, Tetra ethanol Orthosilicate, and HCL
 Performed sol gel process to synthesis chemicals
 Keep accurate records in lab notebook
 Analyze data
 Assist with Design and development
 Perform ball milling, sieving and annealing
 Use HORIBA LA-300 to obtain particle size
 Use veneer calipers to measure parts
 Prepare different substrates by measuring cutting and grinding
 Use SEM to analyze and evaluate chemicals
 Use analytical scale to weigh compounds
12/13/2013 TO 4/2/2014 SendX Medical Incorporated Carlsbad, CA
LAB ASSISTANT
 Responsible for assembling flow cell device
 Prepared solutions and regents
 Performed QC of parts to ensure accuracy
 Perform validation test to ensure correct levels of blood glucose, PH and oxygen levels
 Use particle counter to measure air quality of type II clean room
 Perform wet/dry etching
8/5/2013 TO 12/20/2013 Hologic Rancho Bernard, CA
PRODUCTION ASSISTANT
 Clean and maintain all glassware
 Operate Autoclave and sterilize supplies
 Perform FDA validation test: Ph testing, TOC and conductivity
 Perform CIP and SIP of bulk tanks
 Follow all SOP’s and comply with FDA/cGMP standards
 Assist fill team with change overs and set up of equipment
 Perform swab test to ensure surface areas met FDA a requirements
1/8/2013 TO 6/21/2013 Retrogen San Diego, CA

2. LAB ASSISTANT
 Load DNA samples into 96 well plates with multi-channel pipettes
 Sort DNA samples out
 Enter data into LIMS system
 Use ABI 3730XL equipment to obtain Sanger sequencing
 Clean and maintain lab/ operate autoclave
 Perform PCR reactions
4/2/2012 TO 12/21/2012 Scripps Institute La Jolla, CA
LAB ASSISTANT II
 Responsible for two bottle choice assay
 Kept accurate records in a lab notebook
 Monitor mice by taking body weights
 Do Western Blots, ELISA test and SDS-Page
 Prepare LB broth and agarose plates for bacterial colonies
 Perform transformation and prepare plasmids using Quiagen kits
 Obtain optical density of DNA sample using spectrophotometry
 Prepare gels and do electrophoresis
 Bleed mice and obtain blood alcohol levels
 Enter statistical data into Excel spread sheet and create graphs
 Do tail digest, PCR and genotyping
3/8/2011 TO 12/23/2011 Scripps Institute La Jolla, CA
 LAB ASSISTANT II
 Responsible for testing mice with ABR equipment
 Kept accurate records in a lab notebook
 Monitor mice by taking body weights
 Prepare LB broth and agarose plates for bacterial colonies
 Prepare gels and do electrophoresis
 Do tail digest, PCR and genotyping Do tail digest, PCR and genotyping
LABORATORY EQUIPMENT & SKILLS
DNA QUANTIFICACTION
DNA/RNA EXTRACTION
SPECTROPHOTOMETER
ANIMAIL HANDLING
IP/IM INJECTIONS
GRAM STAINING
AGAR PLATES/LB BROTH
LIMS SYSTEM
HORIBA PARTICLE ANALYZER
CRESSINGTON SPUTTER COATER
PCR
ELISA TEST
SDS-PAGE
FDA REGULATIONS
CGMP/GLP
DNA SEQUENCING
CENTRIFUGE
SEM MICROSCOPE
COMPOUND MICROSCOPE
NANOTECNOLOGY
AUTOCLAVE
PH METERS
TOC TESTTING
CONDUCTIVITY
ELECTROPHORESIS
PIPPETTING
TISSUE CULTURE
PLANATAR BALL MILLING MACHINE
400°C-2000°C FURNACES
°°
RETRO-ORBITAL BLEEDSEDUCATION
6/2/2010 TO 12/23/2010 Cal State San Marcos San Marcos, CA
LAB TECHNICIAN CERTIFICATE

3. 9/14/2001 TO 6/18/2006 Los Angeles Mission College San Marcos, CA
EQUIVALENT TO AA HEALTH SCIENCES
DEANS AWARD FOR GPA MULTIPLE TIMES
COURSE WORK
Microbiology/Lab
Advance Chemistry/Lab
Biochemistry/Lab
Cellular Biology/Lab
Anatomy/Lab
Molecular Biology/Lab
Bioinformatics
Tissue Culture/Lab
Statistics
Physiology/Lab
Genetics/Lab
COMPUTER SKILLS
MICROSOFT OFFICE SUITE, DREAMWEAVER, TYPING, 10-KEY,BIOINFORMATICS