Kent E. Williams has extensive experience in neuroscience research. He holds an M.Sc. in Biology from Indiana State University and has completed coursework toward a Ph.D. in Neuroscience from Ohio State University. His research has focused on extracellular matrix proteins and their implications for angiogenesis and spinal cord injury. He has worked as an adjunct instructor and laboratory director and currently teaches at Vincennes University.

1. CIRRICULUM VITAE
Kent E. Williams, M.Sc.
Current Status:
Address: 9229 Schaeffer Rd
Evansville, Indiana 47720
Telephone: (812) 622-0077
Email: kentewilliams@gmail.com
Education:
Graduate: Ohio State University, Columbus, OH
Completed coursework and candidacy toward a Ph.D. in
Neuroscience, 2010-2013
Research Advisor: Professor Lyn B. Jakeman
Indiana State University, Terre Haute, IN
M.Sc. in Biology, 2010
Research Advisor: Assistant Professor Allan R. Albig
Thesis Title: Extracellular Matrix Proteins: Implications for
Angiogenesis
Undergraduate: Purdue University, West Lafayette, IN
B.Sc. in Biology, 2006
Employment and Research Experience
Adjunct Instructor, Vincennes University, Vincennes, IN, August 2014-Present
I design curriculum, advise, and instruct students on the topic of introductory anatomy
and physiology.
Laboratory Director, IEH, Inc., Washington, IN, March 2014-September 2014
I implemented, maintained, and enforced the quality management system according to
ISO 17025 requirements. This included maintenance and testing of all critical equipment,
purchasing materials for the laboratory, and submitting weekly invoices to the client. I
supervised and worked alongside other analysts in the preparation, analysis, and reporting of
various microbiological and chemical tests. Additionally, I interacted with the on-site client to
ensure smooth operations and to discuss any irregularities or concerns.

2. Adjunct Instructor, Harrison College, Evansville, IN, January 2014-March 2014
I taught a course on medical law and ethics for medical assisting students. This required
curriculum design, instruction, assessment, and advising.
Graduate Research Assistant, Ohio State University, Columbus, OH, April 2011-August 2013
I investigated the timing and distribution of intracellular signaling proteins following
mouse spinal cord injury (SCI) utilizing molecular biological techniques. Using Western blot, I
determined the timing and relative abundance of extracellular signal-regulated kinase (ERK) 1/2
and Akt phosphorylation following mouse spinal cord injury (SCI). To examine distribution of
phosphorylated ERK 1/2 I conducted immunohistochemical labeling in mouse tissue at
numerous times post-injury. Data I generated show that ERK 1/2 activity peaks within hours of
SCI in astrocytes and microglia rostral and caudal to the lesion epicenter. Once the glial scar has
formed, the fibrous astrocytes surrounding the lesion epicenter continue to express
phosphorylated ERK 1/2, suggesting that this intracellular signaling pathway contributes to the
chronic glial scar.
Graduate Research Assistant, Indiana State University, Terre Haute, IN, January 2008-July 2010
I investigated the role of extracellular matrix proteins on tumor angiogenesis utilizing in
vivo and in vitro mammalian systems, as well as microbiological and molecular biological
techniques. Using stable transfection I overexpressed of the matrix protein lumican in murine
pancreatic adenocarcinoma and fibrosarcoma cell lines. I used these cell lines to explore the
effects of lumican on cellular invasion, tumor formation, and proliferation in vitro. Furthermore,
subcutaneous injection of these cells in syngenic mice demonstrated a reduction in tumor size
associated with lumican expression, correlated with reduced angiogenesis and the induction of
endothelial cell apoptosis.
Technical Service Representative, Santa Cruz Biotechnology, Santa Cruz, CA, February 2007-
July 2007
I helped customers troubleshoot problems while using our products. Additionally, I designed
targets for new antibody products and wrote product literature.
Skills:
Microbiology and Biochemistry:
I have had numerous opportunities to isolate RNA from tissues and cells and to design
primers for performing reverse transcription PCR. Additionally, I have experience generating
point-mutations in plasmid DNA using site-directed mutagenesis and analyzing DNA sequencing
results. I am practiced in aseptic technique, protein expression and purification, and gel
electrophoresis.

3. In Vivo:
I have extensive handling experience with rodents, including the administration of I.P.
drugs and collecting pertinent data, including live weight, subcutaneous tumor measurements,
and locomotor behavior. Additionally, I am experienced in post-spinal cord injury rodent care. I
have performed cervical dislocation, carbon dioxide asphyxiation, and anesthetic overdose with
or without exsanguination. I have experience collecting fresh and fixed tissues, and processing
them for protein extraction, cryosectioning, and immunohistochemical labeling. In addition to
mammals, I also have experience in zebrafish maintenance and cross breeding.
Instrumentation and Software:
I have experience operating, calibrating, and maintaining a variety of laboratory
equipment including pipettes, centrifuges, pH meters, scales, electrophoresis chambers,
incubators, and cryostats. Additionally, I rely upon my computer software expertise, including
GraphPad Prism, Microsoft Office, MCID, Endnote, Photoshop, and ImageJ to summarize,
analyze, and present the data I generate.
Personal Strengths:
I am a highly motivated, logical, and independent problem solver with many years of
experience in a research laboratory. My organization and attention to detail are apparent in my
meticulous documentation of my work. I’m a hard worker who sees projects through to
completion.
Publications:
1. Kent E. Williams and Lyn B. Jakeman. ERK 1/2 expression after spinal cord injury:
timing and distribution. (In progress)
2. Lyn B. Jakeman, Kent E. Williams, and Bryan Brautigam. (2014) In the Presence of
Danger: The Extracellular Matrix Defensive Response to Central Nervous System Injury.
Neural Regen Res. 9(4): 377-384.
3. Kent E. Williams, Logan A. Fulford, and Allan R. Albig. (2010). Lumican Reduces
Tumor Growth via Induction of Fas-mediated Endothelial Cell Apoptosis. Cancer
Microenvironment. 4(1):115-126

4. References:
Curt Coffman, Ph.D.
Biology Department Chair
Vincennes University
1002 N. First St
Vincennes, IN
47591
(812) 888-4373
CCoffman@vinu.edu
Lyn B. Jakeman, Ph.D.
Program Director
NIH/NINDS Repair and Plasticity
Neuroscience Center, Rm 2184
6001 Executive Blvd
Bethesda, MD
20892-9525
(301) 496-1447
lyn.jakeman@nih.gov
Allan R. Albig, Ph.D.
Assistant Professor of Biology
College of Arts and Sciences
Science Bldg Rm 117
Boise State University
Boise, ID 83725
Email: allanalbig@boisestate.edu
Tel: (208) 426-1040