1. Baylor College of Medicine
Department of Biochemistry and Molecular Biology,
Alkek N410, Houston, TX, 77054
United States
Phone (lab): 713-798-8432
Phone (cell): 832-859-3000
E-mail: jaemunc@bcm.edu
jaemunc@gmail.com
Jae-Mun Choi, Ph.D.
SUMMARY
Research postdoc experienced in structural biology of various proteins ranging from medically
important molecular chaperons to clinically essential viral proteins. Over 17 years of multidisciplinary
knowledge and expertise including M.S. in molecular biology, industrial experience and Ph.D. in
protein structural biology demonstrated by a major breakthrough achieving 170 citations on a single
paper (458 in total). One of the members of BCM Norovirus research group, which have influenced
the birth of LigoCyte Pharmaceuticals and human clinical trial for Norovirus vaccine development.
EDUCATION & THESIS
Ph.D. - Biochemistry and Molecular Biology, Structural Biology (2012, Sept)
Baylor College of Medicine, USA
Thesis: X-ray structures of Norwalk virus proteins (HBGA-P-domain Complex and viral membrane
modifying protein, p41)
Advisor: Prasad B.V.V. Ph.D.
Master of Science - Molecular Biology (2001, Aug)
Korea University, South Korea
Thesis: Expression of the Aspergillus sp. 5990 phytase gene in Pichia pastoris and comparison of
biological property
Advisor: Jae-Ho Kim Ph.D.
Bachelor of Science (1999, Feb)
Korea University, South Korea
RESEARCH EXPERIENCE
Postdoc Associate, Department of Biochemistry and Molecular Biology, Baylor College of
Medicine at Dr. Prasad Laboratory (2012-present)
 Lead roles in RO1 Grant Funded Project (On going): Structural studies of Norovirus NTPase
using X-ray crystallography
 Lead roles in RO1 Grant Funded Project (On going): Structural studies of Rotavirus NSP1 protein
using X-ray crystallography
 Supporting roles in collaborative Project (Successfully finished): Structural studies of influenza
virus NS1 protein using X-ray crystallography
 Lead roles in developing future RO1 project (In progress): virtual screening of anti-viral drugs
against essential Norovirus proteins and structural validation and optimization.
 Lead roles in developing future RO1 project (In progress): Developing Nano-disc imaging system
for viral membrane proteins using cryo-EM.

2. Ph.D., Department of Biochemistry and Molecular Biology, Baylor College of Medicine at Dr.
Prasad Laboratory (2005-2012)
 Lead roles in RO1 Grant Funded Project (Finished and successfully renewed): Structural studies
of Norwalk virus P-domain and its receptor recognition mechanism using X-ray crystallography
Research Technician II, Department of Biochemistry and Molecular Biology, Baylor College of
Medicine at Dr. Tsai Laboratory (2002-2005)
 Supporting roles in RO1 Grant Funded Project (successfully funded): Structural studies of
molecular chaperon proteins, ClipB using X-ray crystallography and cryo-EM
Research Assistant, Enbiogene Inc. (2001-2002)
 Supporting roles in the research department: Development, production and evaluation of small
molecules which can enhance fish immune system for fish farming industry
Master Degree, Graduate School of Biotechnology, Korea University at Dr. Ja-Ho Kim
Laboratory (1999-2001)
 Lead roles in government funded project: Biochemical studies of phytase from Aspergillus. Niger
including purification of enzymes, over-expression in Yeast expression system and development of
enzymatic assays
 Teaching Assistant in Microbiology Lab & Molecular Biology Lab courses
PROFESSIONAL MEMBERSHIP
American Chemical Society (ACS); Regular member
American Crystallographic Association (ACA); Regular member
American Society for Virology (ASV); Regular member
Korean-American Scientist and Engineers Association (KSEA): Regular member
TECHNICAL EXPERTISES
X-ray crystallography
 Protein crystallization, Data reduction programs (HKL200, iMosflm, XDS, D*Trek), various
phasing methods (MR, MAD and SIRAD), Data refinements (Phenix refine and Refmac), Model
building tool (Coot)
Structural Biology
 Structural data validations (PROCHECK), Structural data analysis (Dali, PISA, Consurf, Pymol),
cryo-EM (EMAN2, IMOD), Protein ligand interaction analysis (LigPlot)
Computational Biology
 Virtual screening of drug library (AutoDock Vina, AutoDock), Molecular Dynamics (Gromac,
NAMD), Computer languages (C, Python, Java, JavaScript), Linux system administration (Fedora,
CentOS), Web server administration (Apach Tomcat, Hadoop, MySQL)
Biochemistry, Molecular & Cell Biology/Virology
 General recombinant DNA technologies (In-Fusion), Heterologous protein over-expression in
various hosts (E.coli, Yeast, insect cell, HEK 293), Industrial scale protein expression and
purification (Jar fermenter 4t), Many protein purification methods (Affinity, Ion-exchange, HIC, Size
exclusion), Fab purification, Wester-blot & Co-Immunoprecipitation, Confocal microscope image

3. acquisition and data processing (ImageJ), M13 Phage display library screening (Plaque forming
assay), Binding affinity measurement (ITC, Biacore), Protein folding (DLS, CD, ThermoFlour)
AWARDS & HORNOR
Poster award at 18th
SCSB Structural Biology Symposium (2013)
Travel grant from American society for virology (2012)
Poster Judge at MVM Retreat (2011)
GenDEPOT best poster award from Korean-American Scientist Engineers Association (2011)
Poster award at Department of Biochemistry and Molecular Biology retreat (2011)
Bioneer award, Korean-American Scientist Engineers Association (2006)
Young Scientist fellowship, National research foundation of Korea (2001-2002)
BrainKorea21 fellowship (1999-2001)
Honors student scholarship (1997-1998)
PROFESSIONAL LICENSE
X-ray Beamline access; Advanced Photon Source, Argonne National Lab (DOE)
GOOGLE SCHOLAR STATISTICS
Publications 9
Citations 458
h-index 6
(https://scholar.google.com/citations?hl=en&user=n49exTMAAAAJ&view_op=list_works&sortby=pubdate)
STRUCTURAL BIOLOGY STATISTICS
PDB Database: 9 X-ray crystallographic structures are deposited
EMDataBank: 4 cryo-EM structures are deposited
BOOK
B.V. Venkataram Prasad, S. Shanker, Z. Muhaxhiri, Jae-Mun Choi, R.L. Atmar, M.K. Estes:
Structural Biology of Noroviruses. Viral Gastroenteritis: Molecular Epidemiology and
Pathogenesis Chapter 3.1. 329-378 (2016) ISBN: 9780128022412
JOURNAL PUBLICATIONS
Jae-Mun Choi, Sompong Vongpunsawad, Budi Utama, Banumanthi Sankaran, Mary K. Estes, B. V.
Venkataram Prasad. X-ray structures of 2C-like proteins implicated in cellular membrane
reorganization. (Submitted)
BV Venkataram Prasad, Sreejesh Shanker, Zana Muhaxhiri, Lisheng Deng, Jae-Mun Choi, Mary K
Estes, Yongcheng Song, Timothy Palzkill, Robert L Atmar: Antiviral targets of human noroviruses.
Curr. Opin. Virol 6, (2016)

4. B. V. Venkataram Prasad, Sreejesh Shanker, Liya Hu, Jae-Mun Choi, Sue E Crawford, Sasirekha
Ramani, Rita Czako, Robert L Atmar and Mary K Estes. Structural basis of glycan interaction in
gastroenteric viral pathogens. Curr. Opin. Virol. 7, 119-27 (2014)
Berenice Carrillo, Jae-Mun Choi, Zachary A. Bornholdt, Banumathi Sankaran, Andrew P. Rice, B. V.
Venkataram Prasad. The influenza A virus protein NS1 displays structural polymorphism. J. Virol. 88,
4113-22 (2014)
Sreejesh Shanker, Jae-Mun Choi, Banumathi Sankaran, Robert L. Atmar, Mary K. Estes, and B. V.
Venkataram Prasad. Structural Analysis of Histo-Blood Group Antigen Binding Specificity in a
Norovirus GII.4 Epidemic Variant: Implications for Epochal Evolution. J. Virol. 85, 8635-45 (2011)
Jae-Mun Choi, Anne M. Hutson, Mary K. Estes, and B. V. Venkataram Prasad. Atomic resolution
structural characterization of recognition of histo-blood group antigens by Norwalk virus. Proc. Natl.
Acad. Sci. USA 105, 9175-80 (2008)
Sukyeong Lee, Jae-Mun Choi, Francis T.F. Tsai. Visualizing the ATPase cycle in a protein
disaggregating machine: Structural basis for substrate binding by ClpB. Mol Cell. 25, 261-71 (2007)
Sukyeong Lee, Mathew E. Sowa, Jae-Mun Choi, Francis T.F. Tsai. The ClpB/Hsp104 molecular
chaperone – A Protein disaggregating machine. J. Struct. Biol. 146, 99-105 (2004)
Jae-Mun Choi, Doo-Sang Kim, Moon-Sick Yang, Hyung-Rak Kim, Jae-Ho Kim. Expression of the
Aspergillus niger var. awamori Phytase Gene in Pichia pastoris, and Comparison of Biological
Properties. J. Microbiol. Biotechnol. 11, 1066-1070 (2001)
CONFERENCE PAPERS
Jae-Mun Choi, Sompong Vongpunsawad, Budi Utama, Mary K. Estes, B. V. Venkataram Prasad.
First atomic resolution structure of a 2C-like protein from +ve ssRNA virus exhibits dynamin-like
features. 5th
International Conference Caliciviruses, Beijing, China (Oct, 2013)
Berenice Carrillo, Jae-Mun Choi, Andrew P. Rice, B. V. Venkataram Prasad. X-ray Structure of
Influenza full-length NS1 show determinates of structural polymorphism among NS1 proteins. 18th
Annual Structural Biology Symposium, Galveston, USA (May, 2013)
Jae-Mun Choi, Sompong Vongpunsawad, Budi Utama, Mary K. Estes, B. V. Venkataram Prasad.
First atomic resolution structure of a 2C-like protein from +ve ssRNA virus exhibits dynamin-like
features – Functional implications in membrane reorganization. 18th
Annual Structural Biology
Symposium, Galveston, USA (May, 2013)
Jae-Mun Choi, Sompong Vongpunsawad, Budi Utama, Mary K. Estes, B. V. Venkataram Prasad.
Cellular localization of Norwalk Virus protein p41 implicated in Golgi membrane reorganization. 31st
Annual Meeting of American Society for Virology, Wisconsin, USA (July, 2012)
Bidadi V. Prasad, Jae-Mun Choi, Sreejesh Shanker, Zana Muhaxhiri, Youngcheng Song, Robert L.
Atmar & Mary K. Estes,. Session 5, Structural Biology and Antiviral Drug Discovery; Structural

5. Biology of Caliciviruses. Noro2012, International Norovirus Conference, Lubeck, Germany (March,
2012)
Jae-Mun Choi, Sompong Vongpunsawad, Budi Utama, Mary K. Estes, B. V. Venkataram Prasad. X-
ray structures of p41 implicated in cellular membrane reorganization. Korean-American Biomedical
Symposium, KSEA South Texas, Houston, USA (Nov, 2011)
Sreejesh Shanker, Jae-Mun Choi, Mary K. Estes, Robert L. Atmar, B. V. Venkataram Prasad.
Structural analysis of the Genotype specific recognition of HBGA in GII.4 variant. 4th
International
Conference Caliciviruses, Santa Cruze, Chile (Oct, 2010)
Sompong Vongpunsawad, Jae-Mun Choi, B. V. Venkataram Prasad, Mary K. Estes. Functional
Analysis and Mapping of residues on Norwalk virus VP2 that interact with the major capsid protein.
4th
International Conference Caliciviruses, Santa Cruze, Chile (Oct, 2010)
Jae-Mun Choi, Anne M. Hutson, Mary K. Estes, B. V. Venkataram Prasad. Atomic resolution
structural characterization of Histo-Blood Group Antigens by Norwalk Virus. 27th
Annual Meeting of
American Society for Virology, NY, USA (July, 2008)
Jae-Mun Choi, Anne M. Hutson, Mary K. Estes, B. V. Venkataram Prasad. Recognition of Histo
Blood Group Antigens by Norwalk Virus capsid protein. 3rd
International Conference Caliciviruses,
Cancun, Mexico (Nov, 2007)
PROFESSIONAL JOURNAL REVIEW
Medicine®
(Peer review medical journal): Invited for review
CONFERENCE TALKS
Annual meeting of American Society for Virology, “Cellular localization of Norwalk virus protein p41
implicated in Golgi membrane reorganization” (2012)
Annual meeting of American Society for Virology, “Atomic resolution structural characterization of
recognition of histo-blood group antigens by Norwalk Virus” (2008)
Third International Conference on Caliciviruses, “Structural specificity of Histo-Blood Group Antigen
recognition by Norwalk Virus” (2007)
REFERENCES
B.V.V. Prasad Ph.D. (Ph.D. mentor)
Phone: 713-798-5686
E-mail: vprasad@bcm.edu
Address: Baylor College of Medicine, Houston, TX 77030, USA
Francis T.F. Tsai D.Phil (Research technician)
Phone: 713-798-8668

6. E-mail: ftsai@bcm.edu
Address: Baylor College of Medicine, Houston, TX 77030, USA
Jae-Wook Jung Ph.D. (Master Degree)
Phone: 616-234-0987
E-mail: JaeWook.Jeong@hc.msu.edu
Address: Michigan State University, MI49503, USA