There is one research paper published in the PNAS science journal about role of Aquaporin 3 mediated H2O2 transport in colonic epithelia. Here I try to explain it in easy way with slides. Enjoy it.
5. Previous studies
In the intestine, the generation of extracellular H2O2 at the
mucosal or serosal surface is carried out by NADPH oxidase
(NOX) enzymes, such as NOX1 and dual oxidase 2 (DUOX2).
(Rada B et al., 2008)
H2O2 is a stable reactive oxygen species (ROS) molecule that acts
as an extracellular and intracellular signal that mediates pleiotropic
effects in tissues, including recruitment of immune cells to
damaged areas and cell migration. (Niethammer P et al., 2009)
mice lacking AQP3 experienced impaired recovery after chemical-
induced colitis (Basuroy S et al., 2010)
33. Infection of C. rodentium cause
accumulation of ROS in colonoids.
Conclusion
34. Experiment – 3(C)
To test whether AQP3 mediates
mucosal inflammatory response after
Citrobacter rodentium infection.
35. Measure of body weight and crypt length
12 day
*P<0.05*P<0.05
n=6 n=6
36. Relative expression of IL-6 and TNF-
m-RNA in isolated ECs
n = 6
At 5 day post infection
**P<0.01
37. At 5 day post infection At 25 day post infection
n=5
n=4
Relative expression of IL-6 and TNF- m-RNA
in isolated ECs from AQP3+/+ and AQP3-/-
**P<0.01
**P<0.01
***P<0.001
NAC : n-acetyl cysteine
38. AQP3 is involved in mucosal inflammatory
response required for subsequent bacterial
clearance and recovery
Conclusion