6. Involves placing
Involves placing
thousands of gene
thousands of gene
sequences in
sequences in
known locations
known locations
on a glass slide
on a glass slide
called a gene chip
called a gene chip
Study the
Study the
expression of
expression of
genes at once
genes at once
MICROARRAY
Complementary
Complementary
base pairing
base pairing
between the
between the
sample and the
sample and the
gene sequences
gene sequences
on the chip
on the chip
produces light
produces light
that is measured.
that is measured.
A sample
A sample
containing DNA or
containing DNA or
RNA is placed in
RNA is placed in
contact with the
contact with the
gene chip.
gene chip.
7. Evolved
from Southern Blot
Miniaturized
microarrays were introduced in
1995 (Science 1995;270:467)
1980s
- better surface technologies, more
powerful robots for arraying, better nucleic
acid dye labeling techniques and improved
computational power and automated
analyzers have vastly improved the power
and efficiency of microarray, while also
lowering the cost of these analyses
History
8. Used
to analyze many different systems,
including the classification of microbes
and human microbial pathogens, cellular
responses to pathogens, drug and toxic
exposures, tumor classification, single
nucleotide polymorphism detection, the
detection of gene fusions, comparative
genomic hybridization, alternative
splicing detection (exon junction
array/exon arrays) and gene expression
profiling via analyzing global mRNA
levels
History
9. Classification of microbes and human microbial pathogens
Classification of microbes and human microbial pathogens
Cellular responses to pathogens
Cellular responses to pathogens
Drug and toxic exposures
Drug and toxic exposures
Tumor classification
Tumor classification
Detection of gene fusions
Detection of gene fusions
22. Immune-related gene expression following WSSV infection
Immune-related gene expression following WSSV infection
Microarray results, together with the results of other shrimp
gene studies, indicated not only the importance of innate
immunity for defense against WSSV, but also the novel
antiviral functions of known and unknown proteins in innate
immunity.
24. Immune-related gene expression following YHV infection
Immune-related gene expression following YHV infection
-Over ten responsive genes were shrimp immune-related
genes
- Five genes showed relatively consistent high expression
throughout the YHV infection
26. Immune-related gene expression following Vibrio infection
Immune-related gene expression following Vibrio infection
-Suggests that different immune modulation responses can be
triggered by different pathogens
-Hemocyanin expression was significantly suppressed in WSSV
treated shrimp, whereas it was up regulated in inactivated
Vibrio
27. Immune-related gene expression following Immunostimulation
Immune-related gene expression following Immunostimulation
- Analysis showed that PG administration up-regulated the
expression of immune-related genes
28. Immune-related gene expression following antibiotic treatment
Immune-related gene expression following antibiotic treatment
-For therapeutic and prophylactic purposes
-Expression of genes in the shrimp lymphoid organ including
those that are involved in immune response were altered after
the administration of antibiotics
29. Immune-related gene expression following antibiotic treatment
Immune-related gene expression following antibiotic treatment
30. Immune-related gene expression following environmental stress
Immune-related gene expression following environmental stress
31. Immune-related gene expression following environmental stress
Immune-related gene expression following environmental stress
-Stress should decrease the ability of shrimp to defend
themselves against pathogens
-Shrimp physical condition, the immune response, and
environmental stress are closely related
32. Conclusion
Effective tool in elucidating and understanding the
mechanisms of shrimp responses to viral and bacterial
infection, antibiotic treatment and environmental stress.
The technique is an indispensable tool in the study of
shrimp and should reveal breakthrough information that
could lead to novel applications
