membrane of cell

1. 1. Review basic concepts and new development
2. Current methodologies and techniques
3. Reading scientific literature
4. Research process
Collect background information
Identify scientific questions
Find experimental approaches
Process and interpret data
Draw conclusions
5. Writing research proposals
Cell Biology I. Cellular structure and function

2. Cell Biology I. Topics
Cell membrane and organelles
Introduction of cell signaling
Membrane trafficking, secretory and endocytic pathways
Cytoskeleton
Cell cycle

3. Cell membrane
Wenxia:
1. The concept
of cells
2. Prokaryotic
cells,
eukaryotic
cells,
mammalian
cells
3. Cell
membrane:
plasma
membrane
and internal
membrane
Wenxia:
1. The concept
of cells
2. Prokaryotic
cells,
eukaryotic
cells,
mammalian
cells
3. Cell
membrane:
plasma
membrane
and internal
membrane

4. The bilayer structure of biomembranes

5. The faces of cellular membrane
Wenxia:
1. Exoplasmic
and cytosolic
face and its
importance
2. Compartmen
t with single
and double
bilayers
3. Different
protein and
lipid
composition
of different
membranes
Wenxia:
1. Exoplasmic
and cytosolic
face and its
importance
2. Compartmen
t with single
and double
bilayers
3. Different
protein and
lipid
composition
of different
membranes

6. Three major classes of membrane lipids
Wenxia:
The relationship
of the head
group, fatty acid
and lipid
composition with
the thickness,
curve and fluidity
of membrane
Wenxia:
The relationship
of the head
group, fatty acid
and lipid
composition with
the thickness,
curve and fluidity
of membrane

7. Gel and fluid forms of the lipid bilayer

8. The fluid mosaic model of cell membrane
Initially proposed by Singer and Nicolson 1972

9. Proteins associated with the lipid bilayer

10. Glycophorin A E. coli Porin
Transmembrane proteins

11. adhesion molecules
glycosylphosphtidylinositol
Proteins with covalently linked lipid anchors
(a) Prenylation (b) Acylation
Ras Src

12. Proteins binds to lipids

13. Lipid rafts
By Susan Pierce,
Nature Reviews
Immunology 2, 96-
105 (2002)

14. Lipid rafts
Atomic force microscopy
Fluorescence
microscopy

15. Organelles of the eukaryotic cell

18. Fluorescence-activated cell sorter (FACS)

19. Dot plot of FACS

20. Purification of cell population by magnetic beads

21. Differential centrifugation is a common first step
in fractionating a cell homogenate

22. Separation of subcellular organelle by equilibrium
density gradient centrifugation

23. Fluorescence microscopy

24. Localization of the B cell antigen receptor in lipid rafts
Cholera toxin B subunits
Anti-BCR antibody

25. Deconvolution fluorescence microscopy
DNA/microtubules/actin filaments

26. Transmission electron
microscopy

27. Scanning electron
microscopy (SEM)

28. Journal discussion
Singer S. J. and Nicolson G. L. The fluid mosaic
model of the structure of cell membrane. 1972.
Science 175: 720.
Harder T., Scheiffele P., Verkade P. and Simons K.
Lipid domain structure of the plasma membrane
revealed by patching of membrane components. 1998.
Journal of Cell Biology 141:929.