1. WhiteSci
Whitehead Scientific
Buying Cell Culture Equipment or Media?
Consider these Promega Companion Products
Quantitate Changes in the number of Dead Cells
™
• CytoTox-Glo Cytotoxicity Assay
™
• CytoTox-Fluor Cytotoxicity Assay
™
• CytoTox-ONE Homogeneous Membrane
Integrity Assay
Assess whether these cells die from Apoptosis
• Caspase-Glo® Assays
®
• ApoONE Homogeneous Caspase 3/7 Assay
™
• DeadEnd TUNEL Assays Biological
™
• CaspACE FITC-VAD-FMK in Situ Marker Safety Hoods
Treat Cells &
Assess Biological Effect
Cell Culture
Quantitate Changes in the number of Live Cells
• CellTiter-Glo® Luminescent Cell Viability Assay
• CellTiter-Fluor™ Cell Viability Assay
®
• CellTiter-Blue Cell Viability Assay
• CellTiter 96® AQueous One Solution Assay
Assess changes in cellular Metabolism
• GSH-Glo™ Glutathione Assay
™
• Proteasome-Glo Assays
™
• P450-Glo Cell Based Assays Co2 Incubators
Western & Eastern Cape Gauteng, KZN, Free State & Limpopo
TEL (021) 944 6460, FAX (021) 949 5478 TEL (011) 894 2214, FAX (011) 894 4583
e-mail penny@whitesci.co.za e-mail luzaan@whitesci.co.za
www.whitesci.co.za
P.T.O

2. Promega Companion Products for your cell culture work
Quantitate Changes in the number of Live Cells Quantitate Changes in the number of Dead Cells
Description Size Cat. # Description Size Cat. #
10ml G7570 CytoTox-Glo™ Cytotoxicity Assay 10ml G9290
Luminescent
CellTiter-Glo® Luminescent Cell Viability Assay 10 x 10ml G7571 protease assay measures “dead cell” protease 5 x 10ml G9291
Quick 10 minute luminescent ATP assay with leaked into culture media from cells with
sensitivity down to the 10’s of cells. Scalable to compromised membranes. 2 x 50ml G9292
96-, 384- and 1536-well plate formats. 100ml G7572
CytoTox-Fluor™ Cytotoxicity Assay 10ml G9260
10 x 100ml G7573 Fluorescent
(Rhodamine 110: 485nmEx/520nmEm) protease 5 x 10ml G9261
CellTiter-Fluor™ Cell Viability Assay 10ml G6080 assay measures “dead cell” protease leaked into
Non-lytic culture media from cells with compromised
fluorescent assay membranes. 2 x 50ml G9262
(AFC; 400nmEx/505nmEm) with 5 x 10ml G6081
sensitivity down to the 100’s of cells. Excellent
choice for multiplexing with bioluminescent assays. CytoTox-ONE™ Homogeneous Membrane 200-800
G7890
Scalable to 96-, 384- and 1536-well plates. 2 x 50ml G6082 Integrity Assay assays
Fluorescent (Resorufin;
560nmEx/590nmEm) assay of LDH activity leaked 1000-4000
20ml G8080 G7891
from cells with compromised membranes. assays
CellTiter-Blue® Cell Viability Assay
Resazurin-based, non-lytic fluorescent assay
100ml G8081
(resorufin; 560nmEx/590nmEm) with sensitivity down Assess whether the cells die from Apoptosis
to the 100’s of cells.
10 x 100ml G8082 2.5ml G8090
CellTiter 96® AQueous One Solution 200 assay G3582 Caspase-Glo® 3/7 Assay 10ml G8091
Cell Luminescent protease
Proliferation Assay assay measuring the cleavage of DEVD-luciferin. 10 x 10ml G8093
1000 assay G3580
MTS-based, non-lytic
colorimetric assay (Absorbance 490nm) with 100ml G8092
sensitivity down to the 1000’s of cells. 5000 assay G3581
2.5ml G8200
Assess changes in Cellular Metabolism Caspase-Glo® 8 Assay
Luminescent protease 10ml G8201
GSH-Glo™ Glutathione Assay assay measuring the cleavage of LETD-luciferin
10ml V6911
Cell-based assay to 100ml G8202
understand oxidative stress through glutathione
levels. Simple protocol does not require removal of 50ml V6912 2.5ml G8210
proteins.
Caspase-Glo® 9 Assay
10ml G8660 Luminescent protease 10ml G8211
Proteasome-Glo™ Chymotrypsin-like Cell Based assay measuring the cleavage of LEHD-luciferin.
Assay
Homogeneous luminescent protease assay 100ml G8212
for assessing the chymotrypsin-like activity of the 5 x 10ml G8661
26S proteosome. 1ml G7792
Apo-ONE® Homogeneous
Caspase 3/7 Assay
Proteasome-Glo™ Trypsin-Like Cell Based 10ml G8760 Fluorescent ((Rhodamine 110: 10ml G7790
Assay 485nmEx/520nmEm) protease assay measuring the
Homogeneous luminescent protease assay cleavage of DEVD substrate.
for assessing the trypsin-like activity of the 26S 100ml G7791
5 x 10ml G8761
proteosome.
DeadEnd™ Fluorometric TUNEL System 60 rxn G3250
10ml G8860 Classic TUNEL kit with fluorescein dUTP.
Proteasome-Glo ™ Caspase-like Cell Based
Assay
Homogeneous luminescent protease assay DeadEnd™ Colorimetric TUNEL System
for assessing the caspase-like activity of the 26S Classic 40 rxn G7130
5 x 10ml G8861 TUNEL kit with biotinylated dUTP and
proteosome.
streptavidin horseradish peroxidase.
10 ml G1180 CaspACE™ FITC-VAD-FMK 50µl G7461
Proteasome-Glo ™ 3-Substrate Cell Based
Assay In Situ Marker
Homogeneous luminescent protease assay Simply add to live cells to label active caspases.
Applications to flow cytometry. 125µl G7462
for assessing the 3-substrate activity of the 26S 50ml G1200
proteosome.
CaspACE, CellTiter-Fluor, CytoTox-Fluor, CytoTox-Glo, CytoTox-ONE, DeadEnd, GSH-Glo and Proteasome-Glo are
trademarks; Apo-ONE, Caspase-Glo, CellTiter 96, CellTiter-Blue, and CellTiter-Glo are registered trademarks of Promega
P450-Glo™ CYP3A4 Cell Based Assay 10ml V8901 Corporation. Products may be covered by existing or pending patents, please visit www.promega.com for more information.
Simple
add-mix-measure assay to assess inhibition or
induction of cytochrome p450 3A4. 50ml V6912 Learn more about the latest products and applications for all
your research needs at www.promega.com
Western & Eastern Cape Gauteng, KZN, Free State & Limpopo
TEL (021) 944 6460, FAX (021) 949 5478 TEL (011) 894 2214, FAX (011) 894 4583
e-mail penny@whitesci.co.za e-mail luzaan@whitesci.co.za
www.whitesci.co.za