Which process uses chemiosmosis? A. Pyruvate oxidation B. Electron transport chain C. Citric Acid cycle D. Glycolysis E. Fermentation Solution Chemiosmosis is the process in which the movement of ions takes place across the semipermeable membrane down their Electrochemical gradient.There would be generation of ATP ( Adenosine triphosphate by the movement of ions Across the membrae.). Hydrogen ions or protons will diffuse from a region of high proton concentration to a region of lower concetration region and a electrochemical cocentration gradient across the membrane can be used to make ATP. This process is related to Osmosis , the diffusion of water across the membrane which is why it is called Chemiosmosis. The process which uses chemiosmosis can be listed as follows. They are 1. Glycolysis 2.Citric acid cycle. 3.Electron transport chain. 4. pyruvate oxidation..