steps on writing a good scientific proposal in toxicology research.

1. How to write a
scientific proposal
Professor Eman Ezz Eldawela
Elsharkawy
• Forensic Medicine and
Toxicology,
• Assiut Univeristy

2. TABLE OF CONTENTS
Title
Back ground
Introduction
Objectives & Significance
Methodology
- Chemicals
- Laboratory animal or cell culture
- Parameters
References
1
2
3
4

3. Scientific proposal
Steps on writing of

4. Title of the study
 It must brief, simple, condensed
and express the nature of the
study in very few words.
.
ex: Mancozeb impaired male
fertility in rabbits with trials of
glutathione detoxification

5. • Back ground
 This part is concerned mainly with the history of an idea
you may chose in your article. In this section, you try to
give the reader a good information about the origin of your
article and to widen his knowledge about the study.
 Remember to make this part short, brief and with
references.
• Introduction
 It must be 2 or 3 pages maximum, brief and concerned
with pervious studies related to your idea of proposal. It
must be accompanied with references.
• Objectives & Significances
 This part concerned with the aim of the study
and you must declare the exact objective of
your proposal and the significance and also the
scientific benefit of your idea. You may prefer
put it in bullet points.

6. The aim
EX: The aim of this study was to
determine:
1. The effect of sub-chronic CPF
exposure on the reproductive system
of male rats
2. To assess whether these effects can
be ameliorated by co-treatment with
GSH.

7. • Methodology
• Chemicals or any material you
may used.
• Laboratory animal or cell culture
or plant extra….
• Parameters

8. EX: Methodology
• Chemicals
Chlorpyrifos (CPF) of purity 96.5%(technical grade)
and 100% pure glutathione (GSH; pure
powdered form) were purchased from Sigma
Chemicals (St. Louis).
• Animals
Sexually mature male Sprague-Dawley rats
(weighing (200–250 g) obtained from the
Laboratory Animals.

9. Experimental design
Group 1 Group 2
Control
CPF 30 mg/kg b.w
+ GSH 100 mg /kg
b.w
CPF 30 mg/kg b.w
For 90 days/ twice
/weekly by gavage
For 90 days/
twice /weekly
by gavage
Group 3
Corn oil

10.  Parameters will be measured in your
proposal
Ex. You may determine enzymes, hormones,
metabolites extra…..
Histopathology
DNA marker
Gene expression
Immune parameters as antibodies titer
Gene expression
 You must stated the exact procedures you may
use in details with references.

11. “
References
 References must be written in the body of text in the
background and introduction section and also after the
procedure in the methodology section, ex. ( Elsharkawy et al.,
2014).
 These references must be cited in the references list at the
end of the proposal.
 There are different ways of the writing of bibliography or the
references list, the usual trending two ways are the
alphabetical or numbering.
Ex1. E. E. Elsharkawy, D. Yahia and N. A. El-Nisr,
Chlorpyrifos induced testicular damage in
rats:ameliorative effect of glutathione antioxidant,
Environmental toxicology, 2014, 29, 1011-1019.
Ex2.. Elsharkawy, E. E., Yahia, D., El-Nisr, N. A
.2014. Chlorpyrifos induced testicular damage in
rats: ameliorative effect of glutathione antioxidant,
Enviro. Toxicol., 29, 1011-1019.

12. CALL-TO-DISCUSSION TIME
End your proposal presentation with an image
related to your protocol idea and add the word of
thanks for the audience and open the time for
discussion
ANY QUESTION ???