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Typical growth curve for Sf9 cells in ESF 921 
• This protein-free medium is designed to 
maximize expression 
• Delivers robust growth in a wide range of 
cell types, including Sf9, Sf21, Tni PRO, High 
Five™ and S2 cells 
• Higher specific productivity 
• cGMP compliant 
• Available in 1L bottles or standard bag sizes 
of 8L, 10L, 20L, 38L, and 50L 
Protein-Free Medium 
for Insect Cell Culture 
Expression Systems LLC 
Insect & Mammalian Cell Culture Media, Systems & Services 
2537 Second Street, Davis CA 95618 
Phone: (530) 747-2035 • Toll-Free: (877) 877-7421 • Fax: (530) 747-2034 
www.ExpressionSystems.com 
ESF 921 
Cat #96-001 
Sf9 cells were inoculated into 50 ml ESF 921 media 
in a 125 ml shake flask at a density of 7.5x105 cells/ 
ml. Typical viability for log-growth suspension cultures 
>97%. Cells appear round, shiny with no clumping.

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ESF 921 - Premium media for insect host cells

  • 1. Typical growth curve for Sf9 cells in ESF 921 • This protein-free medium is designed to maximize expression • Delivers robust growth in a wide range of cell types, including Sf9, Sf21, Tni PRO, High Five™ and S2 cells • Higher specific productivity • cGMP compliant • Available in 1L bottles or standard bag sizes of 8L, 10L, 20L, 38L, and 50L Protein-Free Medium for Insect Cell Culture Expression Systems LLC Insect & Mammalian Cell Culture Media, Systems & Services 2537 Second Street, Davis CA 95618 Phone: (530) 747-2035 • Toll-Free: (877) 877-7421 • Fax: (530) 747-2034 www.ExpressionSystems.com ESF 921 Cat #96-001 Sf9 cells were inoculated into 50 ml ESF 921 media in a 125 ml shake flask at a density of 7.5x105 cells/ ml. Typical viability for log-growth suspension cultures >97%. Cells appear round, shiny with no clumping.