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Most medically important family of non–spore-forming gram-negative rods. Most species are normal flora of the GI tract. Salmonella, Shigella, and Yersinia are not normal GI flora. Major cause of nosocomial infections Diseases include UTIs, gastroenteritis, septicemia, food poisoning, wound infections, peritonitis, pneumonia, and meningitis The family exhibits four serological characteristics: O (somatic) antigen-A cell wall antigen-LPS (heat stable), Used for serological grouping of Salmonella & Shigella. K (envelope) antigen-Capsular antigen (heat labile) H (flagellar) antigen-Flagellar antigen-protein (heat labile), Used to serotype Salmonella. Vi antigen-Capsular antigen of Salmonella Typhi-polysaccharide (heat labile), Role in preventing phagocytosis, may mask O Ag, removed by heating. Enterobacteriaceae are facultative anaerobes, ferment glucose. Positive nitrate and catalase, non-hemolytic. Except for Plesiomonas, they are oxidase negative.
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Gram reaction & characteristics: Gram positive or gram variable bacilli, sore forming, obligate anaerobe, non-motile. brick-shaped rods/box car. Spores rarely seen. Spores are subterminal but difficult to induce. Habitat: Common inhabitant of the colon. Virulence factor: Produces several exotoxins; alphatoxin, the most important, mediates destruction of host cell membranes; enterotoxin inserts and disrupts membranes of mucosal cells; beta-toxin is a cytotoxin. Hemolysin, necrotizing toxin. Disease: Cellulitis, gas gangrene. Alpha toxin (lecithinase) → muscle cell necrosis, degradative enzymes → subcutaneous gas bubbles → crepitus myonecrosis with crepitus (crackles), gangrenous muscles → black fluid exudate leaking from skin. Post-abortion sepsis, abdominal infections, and enterocolitis, septicemia.
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Cholera
1.
by chandni kumari
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4.
Cultural Characteristics 1.Ordinary Media 1.nutrient
agar 2.Mac-conkey 3.blood agar 4.gelatin stab 5.peptone water
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2.Plating media
7.
Identification
8.
9.
Cholera red reaction form
nitrous indole add few drop of conc. sulphuric acid to 24 hours peptone water culture with cholera vibrio raddish pink colour is formed
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Classification
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Biofilm
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THANK YOU!
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