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Lab 9
Prepared by
Samira Fattah
Assis. Lec.
College of health sciences-HMU
General character:
• Rod shape.
• gram-negative.
• motile.
• non-capsulated .
• Possessing peritrichous flagella.
• Non-la...
-Proteus species are found in the intestinal tract
of human.
- Species of medical importance:
• P. mirabilis
• P. vulgaris
P. mirabilis
causes 90% of all Proteus infections in humans.
Clinical features:
• Urinary tract infection
• Septicemia
• A...
P. vulgaris
• Important nosocomial pathogen.
• Isolated in wound infection and urinary tract
infection.
Laboratory diagnosis
Specimen
• Urine.
• Pus.
• blood.
• ear discharge
Gram stain
• Gram-negative rods
Culture characteristic
On blood agar
• Proteus mirabilis does not form distinctive colonies
on Blood Agar, instead the bacteria swarm across the
...
• Discontinuous swarming produces concentric circles
around the point of inoculation.
• Continuous swarming produces a uniform film.
On nutrient agar
On MacConkey agar
• Non-lactose fermenters that may exhibit swarming
colorless colonies with slight swarming
On EMB
Biochemical test
• Indole test
is used to differentiate
P.mirabilis (-ve) from
P. vulgares(+ve)
Urease test
• positive urease (which is the fundamental test to
differentiate Proteus from Salmonella).
• Most strains pro...
Gelatinase test
• Positive result.
• Liquefaction of gelatin by gelatinase enzyme.
• oxidase-negative
• Catalase-positive.
API20E Test
• Proteus mirabilis
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Proteus spp lecture

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lab diagnosis of Proteus sp.

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Proteus spp lecture

  1. 1. Lab 9 Prepared by Samira Fattah Assis. Lec. College of health sciences-HMU
  2. 2. General character: • Rod shape. • gram-negative. • motile. • non-capsulated . • Possessing peritrichous flagella. • Non-lactose fermenting.
  3. 3. -Proteus species are found in the intestinal tract of human. - Species of medical importance: • P. mirabilis • P. vulgaris
  4. 4. P. mirabilis causes 90% of all Proteus infections in humans. Clinical features: • Urinary tract infection • Septicemia • Abdominal and wound infection • Secondary invader of ulcer, burn and chronic discharging ear.
  5. 5. P. vulgaris • Important nosocomial pathogen. • Isolated in wound infection and urinary tract infection.
  6. 6. Laboratory diagnosis Specimen • Urine. • Pus. • blood. • ear discharge
  7. 7. Gram stain • Gram-negative rods
  8. 8. Culture characteristic
  9. 9. On blood agar • Proteus mirabilis does not form distinctive colonies on Blood Agar, instead the bacteria swarm across the surface of the agar. • P. mirabilis produces a very distinct fishy odour.
  10. 10. • Discontinuous swarming produces concentric circles around the point of inoculation.
  11. 11. • Continuous swarming produces a uniform film.
  12. 12. On nutrient agar
  13. 13. On MacConkey agar • Non-lactose fermenters that may exhibit swarming colorless colonies with slight swarming
  14. 14. On EMB
  15. 15. Biochemical test
  16. 16. • Indole test is used to differentiate P.mirabilis (-ve) from P. vulgares(+ve)
  17. 17. Urease test • positive urease (which is the fundamental test to differentiate Proteus from Salmonella). • Most strains produce a powerful urease enzyme, which rapidly hydrolyzes urea to ammonia and carbon monoxide.
  18. 18. Gelatinase test • Positive result. • Liquefaction of gelatin by gelatinase enzyme.
  19. 19. • oxidase-negative • Catalase-positive.
  20. 20. API20E Test • Proteus mirabilis

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