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Relying on the above pathway for biosynthesis of tryptophan, fill in the table below to predict
growth (+) or failure to grow (-) when mutants defective in each of the trp genes are placed on
media supplemented with the compounds listed.
Solution
Answer:
A mutant for a particular gene in the tryptophan pathway will only grow when it is supplemented
with the required nutrient so that the biosynthetic pathway for the tryptophan synthesis is
resumed. The mutant strain (auxotroph) will proliferate only when the medium is supplemented
with the required substance leading to the completion of the tryptophan biosynthetic pathway.
+ indicates growth when a particular supplement is given. Mutants which have dysfunctional
trpB, trpC, trpD or trpE can still grow if supplemented with any of Indole, tryptophan or
Indole+tryptophan because in all of these cases the strain will get the required tryptophan
necessary for its survival.SupplementMutanttrpAtrpBtrpCtrpDtrpENone-----Anthranilic acid----
+Indole-++++Tryptophan+++++Typtophan and indole+++++

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Relying on the above pathway for biosynthesis of tryptophan, fill i.pdf

  • 1. Relying on the above pathway for biosynthesis of tryptophan, fill in the table below to predict growth (+) or failure to grow (-) when mutants defective in each of the trp genes are placed on media supplemented with the compounds listed. Solution Answer: A mutant for a particular gene in the tryptophan pathway will only grow when it is supplemented with the required nutrient so that the biosynthetic pathway for the tryptophan synthesis is resumed. The mutant strain (auxotroph) will proliferate only when the medium is supplemented with the required substance leading to the completion of the tryptophan biosynthetic pathway. + indicates growth when a particular supplement is given. Mutants which have dysfunctional trpB, trpC, trpD or trpE can still grow if supplemented with any of Indole, tryptophan or Indole+tryptophan because in all of these cases the strain will get the required tryptophan necessary for its survival.SupplementMutanttrpAtrpBtrpCtrpDtrpENone-----Anthranilic acid---- +Indole-++++Tryptophan+++++Typtophan and indole+++++