1) Gas production in TSI test is often seen as cracks in the media EXPLANATION If lactose (or sucrose) is fermented, a large amount of acid is produced, which turns the phenol red indicator yellow both in butt and in the slant. Some organisms generate gases, which produces bubbles/cracks on the medium. 2) The LIA tests changes from green to blue when positive EXPLANATION 3)Klebsiella pneumoniae produces a black butt on a TSI test ANSWER YES WE CAN SAW BLACK PRECIPETATION 4) Salmonella typhimurium is gram-positive bacteria EXPANATION Salmonella typhimurium is a pathogenic Gram-negative bacteria predominately found in the intestinal lumen 5. ANSWER The MR-VP media was inoculated using the slant method Procedure for Methyl Red (MR) Test MR-VP broth is used for both MR Test and VP test. Only the addition of reagent differs, and both tests are carried out consecutively. If bacteria grow only on the butt of the biochemical test, then it is considered aerobic. ANSWER YES IT IS CORRECT Solution 1) Gas production in TSI test is often seen as cracks in the media EXPLANATION If lactose (or sucrose) is fermented, a large amount of acid is produced, which turns the phenol red indicator yellow both in butt and in the slant. Some organisms generate gases, which produces bubbles/cracks on the medium. 2) The LIA tests changes from green to blue when positive EXPLANATION 3)Klebsiella pneumoniae produces a black butt on a TSI test ANSWER YES WE CAN SAW BLACK PRECIPETATION 4) Salmonella typhimurium is gram-positive bacteria EXPANATION Salmonella typhimurium is a pathogenic Gram-negative bacteria predominately found in the intestinal lumen 5. ANSWER The MR-VP media was inoculated using the slant method Procedure for Methyl Red (MR) Test MR-VP broth is used for both MR Test and VP test. Only the addition of reagent differs, and both tests are carried out consecutively. If bacteria grow only on the butt of the biochemical test, then it is considered aerobic. ANSWER YES IT IS CORRECT.

1. 1) Gas production in TSI test is often seen as cracks in the media
EXPLANATION
If lactose (or sucrose) is fermented, a large amount of acid is produced, which turns the phenol
red indicator yellow both in butt and in the slant. Some organisms generate gases, which
produces bubbles/cracks on the medium.
2) The LIA tests changes from green to blue when positive
EXPLANATION
3)Klebsiella pneumoniae produces a black butt on a TSI test
ANSWER
YES WE CAN SAW BLACK PRECIPETATION
4) Salmonella typhimurium is gram-positive bacteria
EXPANATION
Salmonella typhimurium is a pathogenic Gram-negative bacteria predominately found in the
intestinal lumen
5. ANSWER
The MR-VP media was inoculated using the slant method
Procedure for Methyl Red (MR) Test
MR-VP broth is used for both MR Test and VP test. Only the addition of reagent differs, and
both tests are carried out consecutively.
If bacteria grow only on the butt of the biochemical test, then it is considered aerobic.
ANSWER
YES IT IS CORRECT
Solution
1) Gas production in TSI test is often seen as cracks in the media
EXPLANATION
If lactose (or sucrose) is fermented, a large amount of acid is produced, which turns the phenol
red indicator yellow both in butt and in the slant. Some organisms generate gases, which
produces bubbles/cracks on the medium.
2) The LIA tests changes from green to blue when positive
EXPLANATION
3)Klebsiella pneumoniae produces a black butt on a TSI test
ANSWER
YES WE CAN SAW BLACK PRECIPETATION

2. 4) Salmonella typhimurium is gram-positive bacteria
EXPANATION
Salmonella typhimurium is a pathogenic Gram-negative bacteria predominately found in the
intestinal lumen
5. ANSWER
The MR-VP media was inoculated using the slant method
Procedure for Methyl Red (MR) Test
MR-VP broth is used for both MR Test and VP test. Only the addition of reagent differs, and
both tests are carried out consecutively.
If bacteria grow only on the butt of the biochemical test, then it is considered aerobic.
ANSWER
YES IT IS CORRECT