Se hace una exposicíon y resumen del estudio

1. Present By:
Válery Michel Acosta Blandón
Ana Sofía Agudelo Ramirez
Universidad Pontificia Bolivariana
Medicina
Semestre III

2. Introduction
Insulin-like growth
factor 1
IGF-1 is the growth factor type 1
insulin, this is a protein made by
the body that stimulates the
growth of many types of cells.
Also IGF-1 has a great cell
differentiation capacity.
It was used in this study
because it improves and
increases the effects
therapeutic of human placental
mesenchymal cells

3. Introduction
How is this gene expressed at
the placental level?
What effect does it have?

4. General Objetive
The present study aimed to
investigate the effect of
insulin-like growth factor-1
(IGF-1)- expressing
placenta-derived
mesenchymal stem cells
(hPMSCs) on healing of burn
wounds.

5. Materials
and Methods
Flow cytrometry
Is a technique that is based
on the detection of a
reaction between an
antigen and an antibody
En este estudio usamos
esta prueba para detectar
marcadores de superficie
celular de MSC.

6. Materials
and Methods
Enzyme-linked immunosorbent assay
(ELISA)
La técnica ELISA es una técnica
útil para la cuantificación de Ag y
anticuerpos.
Se uso para identificar Los niveles
de expresión de citoquinas
proinflamatorias como TNF-α,
IL-1β e IL-6.

7. Materials
and Methods
PCR
The coding sequence of
IGF-1 was PCR amplified.
The polymerase chain reaction is
an in vitro enzymatic reaction that
amplifies millions of times a
specific DNA sequence for several
cycles repeated. if we use genomic
DNA as a substrate, so we typically
talk about a PCR

8. Materials
and Methods
Real Time PCR
Real-time PCR was used to determine the
expression of the target gene at the mRNA level.
β-actin was measured to
normalize the mRNA level of
IGF-1
The expression of IGF-1 at both mRNA and protein
levels were significantly increased in hPMSCs
infected with Lv-IGF-1 compared to that with
LvVector

9. Results
IGF-1 solo, tiene mayor expresión en
comparación con el vector y el control.

10. Results

11. Results

12. Results

13. Discussion
AUTOR Grellner W, et. all. He L, et all. Lu Sw, et all
FUNDAMENTO Increased expression
of these inflam-
matory cytokines was
observed at the
wound site post burn
injury
TGF-β is a key cytokine
to promote tissue
fibrosis, and stimulate
dermal fibroblasts to
synthesize collagen.
Inhibition of collagen
deposition and TGF-
β1 expression can
reduce scar
formation.
CORRECTO O NO

14. Conclusions
IGF-1 enhances the therapeutic effect of hPMSCs on tissue repair by
promoting cell proliferation and differentiation potential into epithelial
cells, as well as inhibiting inflammation and collagen deposition
Overexpression of IGF-1 in hPMSCs reduces the expression of TNF
, IL-1 and
IL-6 in wound healing which makes the wound non-inflamed and improved
the rate scaring
The skin in wound healing expresses vascular endothelial growth factor,
transforming growth factor, type I and type 3 collagen, with hPMSC it was
possible to positively regulate their expression, which helped correct and
fast burn healing

15. Conceptual Maps

16. Conceptual Maps