Basic fibroblast growth factor gel preparation induces angiogenesis during wound healing by accelerating the wound healing process. A rat model with full-thickness skin defects was used to study the effects of bFGF gel. The bFGF gel treatment group showed increased levels of proliferating cell nuclear antigen and collagen, as well as higher expression of angiogenesis promoting factors like VEGF, MMP2, and MMP9. Histopathological analysis also found the bFGF gel increased invasive distance and collagen formation at 7, 14, and 21 days. It was concluded that bFGF gel promotes and accelerates wound healing at different levels, with the greatest impact between 7-14 days after injury.

1. Laura Sofía Cuervo Sterling
Segundo Semestre - Universidad Pontificia Bolivariana
2023-01

2. Introduction
Materials and methods
Results
Discussion
Conclusion
Objectives

3. Introduction
Angiogenesis is defined as the physiological
process by which new blood vessels are formed
from pre-existing blood vessels. It is a complex and
highly ordered process that relies on extensive
signaling networks between and within endothelial
cells (ECs) as well as associated cells such as
fibroblasts that play a highly determinant role in this
process as well as factors such as FGF
(fibroblast growth factor).

4. This study aimed to observe the effect of basic fibroblast growth
factor (bFGF) gel preparation on wound repair in a full-thickness skin
defect rat model and to further explore its mechanism.
Objective

5. Materials and methods
qRT -PCR
animal models and administration

6. Materials and methods
Enzyme-linked immunosorbent assay (ELISA)
Western blotting

7. Results
Figure 2. The histopathological characteristics and the levels of PCNA and Collagen I: (a) HE
staining; (b) Masson staining. Histopathological observations of invasive distance (black arrow)
and collagen formation rises (white arrow) at 7, 14, and 21 days; (c) The levels of PCNA (7d) and
Collagen I (14d) were measured by Western blotting and quantified. Levels of significance are
depicted as: ##p<0.01. ###p<0.001 model group versus normal group; *p<0.05 bFGF gel group
versus control gel group; Values are expressed as mean±SEM, n=3.

8. Figura 6. Effects of bFGF gel on expressions of angiogenesis related factors
at wound site: (a) the levels of VEGF, MMP2, and MMP9 were measured by
Western blotting and quantified

9. Author Affirmation Agree or not
Munaron L
Angiogenesis is a key link in wound healing and the development of
disease. It provides oxygen, nutrition, and bioactive substances to the
wound site and thus plays an important role in wound healing
Veith AP, Henderson
K, Spencer A, et al.
Demidova-Rice TN,
Durham JT and
Herman IM
1.
2.
Vascular endothelial cells line the inner surface of blood vessels and
are the main cell type involved in the formation of new blood vessels
Steffensen B, Häkkinen L
and Larjava H
When MMPs are activated during trauma, they can affect wound
tissue regeneration, repair, remodeling, and scar formation by
participating in the degradation of the extracellular matrix
Discussion

10. Thanks to the exponential development of sciences such as
microbiology, it has been possible to go beyond the knowledge
we have about the human organism, allowing us to contrast this
knowledge with the discovery of of the mechanism of action of
external factors that affect it in different ways, causing serious
damage to its integrity and functioning.
Conclussion
With all the knowledge that this science has given
us until today, it has been possible to develop
projects to combat diseases and / or pathologies
that have seriously affected the health of people
for many years, and even decades. In this way, the
quality of life of people is improved through the
development of new treatments.

11. VEGF MMP2 MMP29 PCNA Collagen I β-actin
Anti-rabbit anti-mouse
VEGF ELISA kit Rat MMP2 ELISA kit Rat MMP9 ELISA kit
The samples were
analyzed by:
Primary ab
Observation of wound healing
Analysis of pathological staining
sections
Quantitative reverse transcription-
polymerase chain reaction (qRT-PCR)
Western blotting
Enzyme-linked immunosorbent assay
(ELISA)
Materials used:
Elisa Kits
Antibodies
Results bFGF can accelerate wound healing
bFGF gel preparation could significantly
increase proliferating cell nuclear
antigen (PCNA, value-added marker)
bFGF promotes skin wound healing
Rats treated with bFGF started to grow
hair in areas where the rest of the rats
did not.
The bFGF group obtained high levels of
expression of angiogenesis-promoting
factors.
Basic fibroblast growth factor gel preparation induces
angiogenesis during wound healing
They established a rat model with a full-layer skin defect. First, conventional experimental methods were used to study the promoting effect of bFGF on wound healing, and then OCTA was used to
observe the wound and further explore the mechanism of action of the gel preparation.
Animals
Temperature: 20–25°C
Relative humidity: 30%–50%
They were adapted to ventilated cages:
Conditions
They were put
under:
A 12 h light/dark
cycle
They had:
Free access to food
and water.
Secondary ab
It was concluded that the use of
bFGF gel can promote and
accelerate wound healing at
different levels. So it was found
that the days where bFGF
treatment has the greatest
impact is between 7-14 days
after injury.
Elaborated by: Laura Sofía cuervo sterling