Diapositivas seminario biología molecular, Integrantes: Mateo Lopez y Jasmin Lim.

1. Presented by: Mateo López and Jasmin Lim
Semester: Third
UPB

2. Introduction
Ovarian cancer

3. The Relation between IGF1R and Cancer
● Insulin-like growth factor (IGF)
● Regulate growth and development of multiple cancer types

4. Objective
Provide evidence that IGF1R Axis Inhibition Restores Dendritic Cell Antitumor
Response in Ovarian Cancer.

5. Methods
Immunohistochemistry
- Tissue Microarray (TMA): study the expression of various genes in the same time

6. Western Immunoblots
- Cells treated with 5 μM of NVP-AEW541
- Cells were harvested
- washed twice with PBS
- incubated with lysis buffer for 20 minutes.
- centrifuged at 13,000 rpm for 10 minute
- boiled for 5 minutes.

7. Flow Cytometry
- Size.
- Granularity and structural
complexity.
- Fluorescence.
- Differentiated DCs targeted
with:
. CD11b
. CD141
. IGF1R
. pIGF1R

8. Study population
1- 36 patients with EOC.
2- All of them operated in Hillel Yaffe Medical Center during 2012-2018.
3- No further criteria were applied to select the patients.

9. Results

11. Scratch Assay:
- DCs---->pretreated with an IGF1R inhibitor + Ovarian cancer cells (ES2)
15% reduction 26.5% reduction

12. p: 0.05

13. Discussion
AUTOR DISCUSSION
T.J. Smith “Insulin-like growth factor-i regulation of
immune function: a potential therapeutic
target in autoimmune diseases?”
D. Liu, et al “The stimulation of IGF-1R expression by
Lewis(y) antigen provides a powerful
development mechanism of epithelial
ovarian carcinoma.”
J. Yuan, Z. Yin, K. Tao, G. Wang, J. Gao “Function of insulin-like growth factor 1
receptor in cancer resistance to
chemotherapy. ”

14. Conclusions
1- Molecular techniques are useful in Medicine to provide evidence for hypothesis
that are built based off of clinical observations.
2- The use of a control sample, in any experiment, is fundamental to identify the
actual cause of the change in a variable.
3- Molecular techniques are ones most relevant investigations may not be carried
out well without.

15. Epithelial
ovarian
cancer
most lethal
gynecologic
malignancy
IGF
regulating
growth
invasiveness
receptor (IGF1R)
antiproliferative activity of EOC
cells
regulate DC
maturation.
Increase
tumor infiltrating lymphocytesEOC patient survivalinhibitor
immunotherapy
immune response
block
induce
DC dysfunction
expressing immunosuppressive factors
- arginase I
- IL-10
- TGF-β
- VEGF T
can
impair
differentiation
maturation
function of
the host DC