I Aman Thakur prepared centrifuge presentation during my M.phil .

2. CONTENTS
Introduction
Principle
Working
Types of Centrifuges
Types of rotors
Uses Of centrifuge

3. INTRODUCTION
A centrifuge is a device used for separating
particles from the solution
or a device which puts anything into spin
on its axis.

4. Three forces:
Gravitational
force
Centripetal
force
Centrifugal
force

5. ROTORS:
Fixed angel
rotor
Swinging
bucket
rotor

6. FIXED ANGLE ROTORS & SWINGING BUCKET

7. ON WHAT PRINCIPLE CENTRIFUGE WORKS?
Centrifuge works on the Principle of
‘SEDIMENTATION’, “which states that in a solution
whose density is higher than that of the solvent
sink(sediment) and particles that are lighter than it
float to the top”.

8. HOW CENTRIFUGE WORKS?
Imagine a mixture where there are two different sized
particles.
As it spins ,
the larger particles remains at the top
smaller particles stay close to the centre.

10. TYPES OF CENTRIFUGE:
Small
benchtop
centrifuge
1
Micro
centrifuge
2
High speed
centrifuge
3
Ultra
centrifuge
4

11. Small benchtop centrifuge:
with or without refrigeration
Slow speed(eg; upto 4000RPM)
Common in clinical labs.
Can take approx.(upto)100 tubes ,
depending upon diameter.

12. •Microcentrifuge:
-Take tubes of small volumes (upto
2ml)
-can generate forces upto ~15,000*g
-with or without refrigeration
-very common in
biochemistry/biological
labs/molecular biology.

13. •High speed centrifuges:
-15,000-20,000 RPM
-Large sample capacity
-normally refrigerated
-research applications.

14. •Ultracentrifuge:
- 65,000RPM
-limited lifetime
-require special rotors
-care in use – balance critical
- Research applications.

15. USES OF CENTRIFUGE:
Use of centrifuge in a lab is noticeable because without a
laboratory centrifuge the research task becomes impossible.
Simply centrifuges are used in biology, chemistry and
biochemistry for separation.
Urinalysis in the medical laboratory also finds a great use of
the centrifuge.