An overview of PomBase curation and protein characterization

1. 1
Antonia Lock, Midori A. Harris, Kim Rutherford, Jürg Bähler, Stephen G. Oliver, Valerie Wood
Community
Genome Organiza1on
Fission yeast laboratories
Data Mining
with
Pombelist subscribers
Unique monthly database users
S. pombe researchers
1000 people
“The taste is somewhat like soured wort of the smallest descrip5on, but
strangers, who at first dislike it exceedingly, are soon reconciled to it by the
pleasurable sensa5ons to which it gives rise…”
– Richard Francis Burton (1860)
Transmembrane
transport 283
Protein trafficking
& cell surface 769
Signalling 293
Broad eukaryo1c (fungi to human)
Fungi only
Schizosaccharomyces only
Ancestral (broad eukaryo8c & archaea or prokaryote)
5071
proteins
255
bortezomib
sensi1ve
752
mitochondrial
950 with
TM domain
134
3473 conserved
in human
172
5
Protein Characteriza1on
Pombe, or beer in Swahili, is the tradi8onal inebriant of
Eastern African countries. Fission yeast was described by
Paul Lindner in 1893, and Urs Leopold isolated the standard
lab strains in the 1940s.
Mito1c cell cycle 436
2000
3000
4000
5000
Unknown
process
Process
inferred from
homology
Experimentally
characterized
process
213,000 annota1ons
Literature & Annota1on
Community curated
PomBase Curated
Uncurated
Transla1on 489
Small molecule
metabolism 490
Other 573
Taxonomic
conserva1on of
processes
Transcrip1on,
RNA processing 938
Unknown 830