En esta exposición se hace referencia a un artículo en el que se estudió la relación entre el MicroRNA 197-3p y el daño en células endoteliales de arteria coronaria en niños con enfermedad de Kawasaki.

1. VALERIAVILLADA ORTIZ. SEMESTRE III. UNIVERSIDAD PONTIFICIA BOLIVARIANA

2. KAWASAKI DISEASE.
 Inflammatory vasculitis, which affects children
younger than 5 years old. Nowadays it’s known as
the principal cause of acquired heart disease in
children.
 Clinical manifestations are: persisten fever,
polymorphous rash, oropharingeal changes,
conjunctival inyection or conjunctivitis,
lymphadenopathy.
 Treatment: intravenous G inmunoglobuline, aspirin.
 Prevalence: 175 per 100.000 of children younger
than 5 years old.

3. MICRORNA 197 3P
 Micro RNA’s are non coding nucleotides secquences,
which bind to mRNA and inhibit its translation into
proteins.
 They have been related to pathophysiology of some
diseases, as hepatic cancer or uterine leiomioma.
 In Kawasaki’s disease, investigators have found a
relation among MicroRNA 197 3p, cardiovascular
injury markers, and lesions.

4. GENERAL OBJECTIVE.
 “We identify a role of miRNA-197-3p in the mechanisms regulating cardiovascular endothelium injury
of patients with KD”.
Chaowu liu et al. MicroRNA 197-3p, mediates damage to human coronary artery endothelial cells via targeting
TIMP3 in Kawasaki disease.

5. MÉTODOS.
PCR-RT.
 Secuencia de RNA, transcriptasa
inversa, DNAc, PCR normal.
 Se realizó para averiguar qué cantidad
de RNA con presencia de Mir 197-3p
había. Se analizaron miméticos e
inhibidores.
MTT
• Fundamento: reactivo MTT, se introduce
a la muestra, las células lo metabolizan
con enzimas del citocromo y NADPH,
formando cristales de formazán.
Espectrofotómetro a 570nm
• Se utilizó para evaluar la viabilidad de las
células. Luego de exponerlas al MIR 197
3p.

6. MÉTODOS.
 Western blot.
 Se realiza electroforesis de una proteína, se le
añaden anticuerpos, y a ellos fluorocromos o más
anticuerpos para visualizarla.
 Se realizó para analizar el suero tanto de los niños
sanos como los pacientes de enfermedad de
Kawasaki, y de los ratones, buscando anticuerpos
o señales de daño endotelial.
• Transfección de plásmidos: consiste en introducir
material genético en una célula por medios como
electroporación o en este caso con un vector
como un virus.
• En el estudio se introdujo una copia del gen
TIMP3, y en algunos casos un inhibidor. Lo que
más adelante en el estudio permitió analizar
cómo se comportaban las células cuando ´no
teníanTIMP3,

7. RESULTADOS.
 Figura 4.
• Figura 5.

8. RESULTADOS.
 Figura 6.

9. DISCUSSION
Autor and article. Cite Comparison with the
article
Monitoring of the serum proteome
in Kawasaki disease patients before
and after inmunoglobulin therapy . Li
Zhang et al.
“The causal pathogen is unknown,
therefore specific diagnostic
biomarkers and therapy are unavailable.
The article agrees with this in that the
pathogen and main cause of Kawasaki
disease is still yet unknown.
Differential role of TIMP2 and TIMP3
in cardiac hypertrophy, fibrosis and
diastolic dysfunction. Dong Fan et al
““TIMP2 and TIMP3 play fundamental
and differential roles in mediating
pathological remodelling, independent
from their MMP-inhibitory function.””
Agrees with the article in that TIMP3
and TIMP2 have role in mediating
remodelling of matrix.
Messenger RNA and microRNA
transcriptomic signatures of
cardiometabolic risk factors.
David Mc Manus et al.
““Circulating miRNAs associated with
multiple cardiometabolic traits
miRNAs miR-505-5p, miR-197-3p, miR-
145-5p, and miR-328 exhibited
significant associations with BMI, SBP,
DBP, and TG”
Agrees with the article in the fact that
miR’s can be asociated with damage to
cells, also adds the caracteristic of
cardiometabolic risk factors.

10. CONCLUSIONS.
 The progress in tecniques and tecnology for
molecular biology investigation allows new
approaches to pathologies and their molecular
fundamentations. For the futures this can expand
knowledge about diseases and improve the
treatment
 The new information about diseases that has been
found with molecular biology investigation can lead
to the creation of new diagnostic tecniques or
therapeutic options that are effective, and
affordable for more people.