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Conference 2OlO :Y-
17. Inflammaory Processes
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Icelandic ash from the Eyjafjallajokull volcano alters T cell tralficking along a highly organized mucosal dendritic cell
eJveolarmacrophage phenotype network
Lmda S. Powers, Gunnar Gudmundsson,Anna Reisetter, Jonas CassieXul, Yuelei Shenr, Dan R Littman3, Michael L Dustin3, Peter
Bairusaitis. Vicki Grassian,Martha M. Monick Yelazquezt2
t
L-nnersin qf lowa, Carver College of Medicine, Iowa City, tUstti-SA, Micro/Immuno, South Bend, (Jnited States,2Notre Dame,
L'ntred States Bio Sci,Notre Dame, [.]nitedStates,'NYU-SoM, Skirball Inst., NY,
United States
Erposure to particulates,including cigarette smoke and
enrirwmsntal polhrtants, alters macrophage function. To The gut associatedlymphoid tissue maintains homeostasiswith !
et-alure particulates from the recent volcanic eruption at the enteric flora while remaining primed to mount a protective response
Ellafallajokull volcano in Iceland, ash was collected from three against pathogens.Many studies have utilized intravital microscopic
different sites proximal to the volcano. Elemental analysis approaches to describe regulation of immunity in secondary
shos'ed metal aggregates within the ash particles and a decrease lymphoid organs. Fewer have examined immune regulation at
in Cr and Ni amounts with increasing distance from the source. effector sites. Here, we develop surgical approachesto apply
I-nash exposed macrophages,electron microscopy showed intravital microscopy to the intestinal mucosa. Using animals with a
phagocytosisand sequestrationofash within vesicles. Endolytic targeted replacementof a single allele of cx3crl with egfu, we report
vesicles containing ash particulate matter were douBle walled, a highly ordeied mucosal dendritic cell network which colocalizes
consistentwith autophagosomes. LC3-II protein levels, a marker with non-hematopoietic elements.DCs within the network actively
of autophagosomes, were increased.All three particles increased probe the surrounding microenvironment while DC trafficking is not
phosphorylation of eIF2a, a stress marker that signals lowered seen during homeostasis. Effector and regulatory Tcell populations
protein translation rates. In conclusion, alveolar macrophages actively migrate through tissue, albeit with distinct behavior.
respondedto ash exposureby phagocytosing small particles and Together, this study highlights the extent and dynamic nature ofthe
delivering them to autophagosomes, contributing to altered mucosal pC network and suggeststhe presenceof a mucosal
signaling cascades. This work suggeststhat inhalation of *highway" which facilitates interaction between DCs and, effector
volcanic particulate matter may alter the ability of alveolar and regulatory T cells. Current studies are aimed at identiffing the
macrophagesto maintain a pathogen free lung. Funding source: contribution of mucosal DCs and non-hematopoietic cells to
NIH R01HL079901, NIH R01HL07743 I and NCRR 3 ULI homeostatic T cell traffickine.
RR024979 ,r
r80 Notes:
The Role of Pro-inflammatoryCltokines and the Vascular
EndothelialGrowth Factor and its Receptors influencing
in
LJmphangiogenesis Filarial and Cancer Subjects -
in
Magapu SolomonSudhakar', SruthiChandran',Divya Oppath
Sivasankaranl, Subash 2,
Babu Subbaraman K. Alaalasundrum3
' RajalalrshmiEngineeringCollege,Thandalam, Departmentof
Biotechnology, CHENNAI,India, "SAlC-FrederickInc., Clinical
Monitoring Res earchProgram, Chennai,India, 3Government
GeneralHospital,Plastic SurgeryDepdrtment,Chennai,India
Filarialparasites affectthe lives ofmillions ofpeople,especially
thoseliving in tropical countries.Cytokinescanenhance
immunogenicityandpromotetumor regression. Likewise, the
role ofthe lymphaticsystemin lynrphaticdiseases received
has
renewed interestdueto the discoveryof lynrphaticmarkerssuch
asVEGF-C, VEGF-D andtheir receptorVEGFR-3.VEGFs
influencelymphangiogeneisis promotetumor regression.
and Our
preliminaryresultsshowthat the level of expression ILI is the
of
samein both filarial and cancerpatients.TNFa levels remained
low in both within andamongfilarial and cancerpopulations.IL-
6levelswerehigh in cancer patients
compared its level in
to
filarial patients.In addition,we found VEGF-D hasa higher
levelofexpression cancer
in subjectsthanin filarial cases.
VEGFR-I showedlower expression filarial patientswhile
in
VEGFR-2 expression levelsremainedthe sameamongfilarial
andcancerpatients.
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