2. INTRODUCTION
WHAT ARE MICRO
RNA,S?
They are endogenous, single-stranded noncoding RNAs that are
implicated in multiple cellular processes through binding to the 3-
untranslated regions of target messenger RNAs for translational
repression or degradation.
3. SEPSIS AND ITS RELATION
WITH THE STUDY
INTRODUCTION
miR-1224-5p is famous for its role in several human
cancers, as well as being involved in the regulation of
the inflammatory response and the generation of ROS.
5. MATERIALES Y MÉTODOS
WESTERN BLOT ELISA
Determinación de las
concentraciones de proteína total.
Proteínas de 20 microgramos se
separaron mediante SDS-PAGE y se
transfirieron a las membranas de
PVDF.
Membranas se incubaron con
anticuerpos secundarios
conjugados con peroxidasa de
rábano picante durante 1 hora.
Detectar las citocinas
inflamatorias en BALF o
homogeneizados de pulmón.
Evaluar las actividades de NF- κ B in
vivo e in vitro, se prepararon
extractos nucleares y se
sometieron a incubación.
6. MATERIALES Y MÉTODOS
PCR LUCIFERASA
El ARN sepreparó
utilizando el
reactivo TRIzol.
Se transcribió de
forma inversa a
ADNc.
El PPAR- γ 3 - UTR se clonó y
se insertó en el plásmido
pGL3.
Se transfectó a las células
HEK293T con o sin el
tratamiento miR-1224-5p
agomir.
9. DISCUSSION
AUTOR
AUTOR´S
CONCLUSION
¿AGREE?
Infectious etiologies are the
common causes of ALI,
especially the bacterial
pneumonia.
E. Fan, D. Brodie y AS
Slutsky
AMPKα is well known as an
energy sensor in eucaryotic
cells and plays critical roles
in controlling systemic
metabolic homeostasis.
H. Endo, S. Owada, Y. Inagaki,
Y. Shida, and M. Tatemichi
K. Aquilano, F. Sciarretta, R.
Turchi et al.
The NLRP3 inflammasome
functions as a molecular
scaffold for the maturation
and release of IL-1β and IL-18.
P. Wan, W. Su, Y. Zhang et al
Z. Wang, G. Xu, H. Wang et al
Q. Liu, L. Y. Su, C. Sun et al
10. CONCLUSSION
Thanks to this type of studies,
the scientific community can
combat diseases by
manipulating molecules as
small as RNA that have an
impact on such important
physiological changes.
MicroRNA-1224-5p plays a
fundamental role in the regulation
of the inflammatory response and
the production of reactive oxygen
species (ROS), which is why a
focus on this data can
considerably improve the damage
caused by this disease.