This document reports on an experiment to determine the maximum wavelengths of light absorbed by a carrot solution using UV-visible spectrophotometry. A carrot sample was crushed and mixed with isopropyl alcohol before placing in a quartz cuvette and running the solution on a spectrophotometer. Absorbance readings were taken at various wavelengths between 200-800nm. The data collected showed peaks at 8 different wavelengths, indicating those were the maximum wavelengths absorbed by the carrot solution. Therefore, the experiment allowed identification of the most prominent wavelengths in the spectrum of the organic carrot solution.

1. A Report
on
Determination of maximum
wavelengath of a given solution
By using UV – Visible
Spectrophotometry
 SAURABH BISWAS ( 58 )
 RAVI RAJPUT ( 79 )
 VISNU KUMAR (74)
 RITVIJ SRIVASTAVA (55)
 RISHAB MATHUR (53)
University of Petroleum &
Energy Studies

2. PURPOSE– To Determine
theMaximum wavelength of a given
solution using Ultra -violet visible
spectrophotometry.
Instruments Required –
chemical Reagent(ISO PROPYL
ALCOHAL), Quartz cubet, , milipipette,
Spectrophotometer, carrot.
PROCEDURE –
 Take two quartz cubet and fill it with
isopropyl alcohal then run baseline for
isopropyl alochal and set it for zero.
 Calculate the wavelength of isopropyl
alochal filled in the quartz cubet.

3.  On other hand take a bit of carrot and
pulp it in the pharell, after crushing it
add 1 ml of isopropyl alochal and then
again mix it.
 Take the above mixture with the help of
milipipette in one of the quartz cubet,
then put it into the spectrophotometer
 And calculate the wavelength.
DATA –
S.no p/v wavelength(nm) abs
1 663.5 0.023
2 475 0.166
3 448.5 0.19
4 425.5 0.185
5 343 0.594
6 308 0.482
7 251 0.851
8 242 0.854

5. ANALYSIS
 Rays of different wavelength are
present in the mixture. But generally
we take wavelength from 800 nm
upto 200nm.
 From 800nm to 400nm the spectrum
is visible and from 400nm to 200nm
the wavelength is Ultraviolet.
RESULT:
Since the rays of maximum wavelength
is present in the spectrum. From the

6. graph get all the peak values for
maximum wavelength. For carrot
solution in isopropyl alcohal we got 8
maximum wavelengths.
CONCLUSION:
Hence we found that the rays
of maximum wavelength are
present in the given organic
solution.
THANKYOU