Escozine for Pets™ has 4 major production steps.
1. Collection of Scorpions from the Scorpion Reservation. 2. Extraction of venom, purification and therapeutic dose preparation. 3. Polarization of extract and quality control of Polarization 4. Manufacturing, quality control, warehouse and shipment.
Mycotoxins are strictly regulated around the world because of their strong carcinogenic effects. A simple and reliable method to analyze mycotoxins is required to ensure food safety. The current methods require time-consuming sample pretreatment. This presentation reports on a fully automated online sample extraction and analysis of mycotoxins in foods by online SFE-SFC-MS.
The automation of sample preparation has become an increasingly important component for reproducible and operator-independent experiments. This work outlines novel strategies that are being utilized for automated online and offline sample preparation to achieve specific goals, such as a host of applications including targeting post-translationally modified proteins, non-tryptic peptides, and intact proteins.
The presentation describes the automated process of the system and present a number of applications from sample matrices such as food, polymers, and pharmaceuticals to show the utility of the system.
A Method for the Quantification of Ethanol Content in Consumable Fruit Juices...PerkinElmer, Inc.
Production of alcohol has been long established in society with many styles that take advantage of the metabolism of sugars into ethanol. While the production of ethanol is desirable for alcoholic beverages, it is undesirable for other beverages which contain sugars that do not wish to be sold as an alcoholic beverage. Such sugar metabolism is naturally occurring and is well understood to happen in raw fruit as well as processed juice and can vary by type, variety and maturation in the growing season. A new application has been developed in the accurate determination of ethanol content in samples of these products utilizing the PerkinElmer® TurboMatrix™ headspace (HS) autosampler for better reproducible results.
Join the experts as they discuss the use of accelerated solvent extraction and QuEChERS techniques for the extraction of pesticide residues from a diverse range of food samples. Tips and tricks for improving the extraction efficiency will be covered, along with selection criteria for each technique by sample type, assisting analysts in modifying existing methods or developing new methods to tackle their analytical challenges
Mycotoxins are strictly regulated around the world because of their strong carcinogenic effects. A simple and reliable method to analyze mycotoxins is required to ensure food safety. The current methods require time-consuming sample pretreatment. This presentation reports on a fully automated online sample extraction and analysis of mycotoxins in foods by online SFE-SFC-MS.
The automation of sample preparation has become an increasingly important component for reproducible and operator-independent experiments. This work outlines novel strategies that are being utilized for automated online and offline sample preparation to achieve specific goals, such as a host of applications including targeting post-translationally modified proteins, non-tryptic peptides, and intact proteins.
The presentation describes the automated process of the system and present a number of applications from sample matrices such as food, polymers, and pharmaceuticals to show the utility of the system.
A Method for the Quantification of Ethanol Content in Consumable Fruit Juices...PerkinElmer, Inc.
Production of alcohol has been long established in society with many styles that take advantage of the metabolism of sugars into ethanol. While the production of ethanol is desirable for alcoholic beverages, it is undesirable for other beverages which contain sugars that do not wish to be sold as an alcoholic beverage. Such sugar metabolism is naturally occurring and is well understood to happen in raw fruit as well as processed juice and can vary by type, variety and maturation in the growing season. A new application has been developed in the accurate determination of ethanol content in samples of these products utilizing the PerkinElmer® TurboMatrix™ headspace (HS) autosampler for better reproducible results.
Join the experts as they discuss the use of accelerated solvent extraction and QuEChERS techniques for the extraction of pesticide residues from a diverse range of food samples. Tips and tricks for improving the extraction efficiency will be covered, along with selection criteria for each technique by sample type, assisting analysts in modifying existing methods or developing new methods to tackle their analytical challenges
Today’s analytical laboratory is faced with tight deadlines to produce results from testing environmental samples. Too often, solid-phase extraction (SPE) presents a bottleneck in the analytical testing process and may cause poor analyte recoveries and highly variable. Despite advances in analytical instrumentation, sample prep often relies on tedious, manual, and expensive techniques such as liquid-liquid extraction.
Sample preparation of environmental water samples can be automated, however.. Use of automated sample preparation addresses the many challenges that laboratories face when preparing samples and can help improve sample processing turnaround times.
Chromatography presentation goes with this free on-demand webinar. Link to webinar: https://event.on24.com/eventRegistration/EventLobbyServlet?target=registration.jsp&eventid=832348&sessionid=1&key=7401504685427A0804ABBD1F956E617C&partnerrefthermo=undefined&sourcepage=register
This presentation evaluates ASTM D7979-16 for the “direct” analysis of 30 PFCs and compares data to the solid-phase extraction EPA drinking water Method 537.
With increasing pressure of a higher sample throughput and fewer chemists, purification labs in medicinal chemistry groups need to be more productive now than ever before.
This presentation will describe a technique that allows the analyst to obtain a higher purity and better resolution using information from the preliminary analytical screening of these samples prior to purification.
This high performance liquid chromatograph (HPLC) is the first-ever instrument designed specifically for quantitative determination of cannabinoid content. Ready for use after one day of installation and testing, it provides a choice of three different HPLC methods and a dedicated user interface for a simplified workflow.
Change of Peptides and Free -Amino Acids Contents during Nanjing Dry-Cured Du...Agriculture Journal IJOEAR
— In order to explore the relationship between the change of peptides and free-amino acid (FAA) and its unique flavour, Dry-cured duck samples of different processing phases were used to study the change of free-amino acid by High Performance Liquid Chromatography (HPLC) in this paper, meanwhile the trichloroacetic acid precipitation method for modeling use to establish the quantitative predicated peptides. The changes of small peptides and free amino acids in the process were studied. The results showed that the level and amount of proteolysis increased with the processing time at traditional technology, meanwhile the amount of peptides were positively correlated with FAA contents (R 2 =0.86).
Determination of Insoluble Solids in Pretreated BiomassBiorefineryEPC™
Determination of Insoluble Solids in Pretreated Biomass
DISCLAIMER:
YOU AGREE TO INDEMNIFY BioRefineryEPC™ , AND ITS AFFILIATES, OFFICERS, AGENTS, AND EMPLOYEES AGAINST ANY CLAIM OR DEMAND, INCLUDING REASONABLE ATTORNEYS' FEES, RELATED TO YOUR USE, RELIANCE, OR ADOPTION OF THE DATA FOR ANY PURPOSE WHATSOEVER. THE DATA ARE PROVIDED BY BioRefineryEPC™ "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING BUT NOT LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE EXPRESSLY DISCLAIMED. IN NO EVENT SHALL BioRefineryEPC™ BE LIABLE FOR ANY SPECIAL, INDIRECT OR CONSEQUENTIAL DAMAGES OR ANY DAMAGES WHATSOEVER, INCLUDING BUT NOT LIMITED TO CLAIMS ASSOCIATED WITH THE LOSS OF DATA OR PROFITS, WHICH MAY RESULT FROM ANY ACTION IN CONTRACT, NEGLIGENCE OR OTHER TORTIOUS CLAIM THAT ARISES OUT OF OR IN CONNECTION WITH THE USE OR PERFORMANCE OF THE DATA.
NOVEL IPQCL AND FPQC TEST FOR OPTHALMIC PREPARATION AS PER IP, BP A...roshan telrandhe
Ophthalmic preparation are the sterile liquid or semisolid preparation meant to installation in to the eyes in the space between eye lids and eye ball .
These product must be sterile and are prepare under the same condition as that of parenteral preparation
The presence of Per- and Polyfluorinated Alkyl Substances (PFAS) in drinking water is being thoroughly studied due to the persistence of these compounds in the environment and their potential health effects. However, there is limited knowledge about the occurrence of these chemicals in bottled water, despite the increasing concerns about PFAS in the food supply. This poster shows results from a fast and simple direct injection method similar to draft EPA method 8237, using the Shimadzu triple quad LCMS-8050 to analyze seven commercially available samples of bottled water for 24 PFAS.
This is an Engg Biotechnology project based on medicinal plant i.e singapore cherry or jamaican cherry tree (scientific name Muntingia calabure ), we did in 2013 in GMIT college Davangere, karanataka, India. i have complete project detail what we did..,
Today’s analytical laboratory is faced with tight deadlines to produce results from testing environmental samples. Too often, solid-phase extraction (SPE) presents a bottleneck in the analytical testing process and may cause poor analyte recoveries and highly variable. Despite advances in analytical instrumentation, sample prep often relies on tedious, manual, and expensive techniques such as liquid-liquid extraction.
Sample preparation of environmental water samples can be automated, however.. Use of automated sample preparation addresses the many challenges that laboratories face when preparing samples and can help improve sample processing turnaround times.
Chromatography presentation goes with this free on-demand webinar. Link to webinar: https://event.on24.com/eventRegistration/EventLobbyServlet?target=registration.jsp&eventid=832348&sessionid=1&key=7401504685427A0804ABBD1F956E617C&partnerrefthermo=undefined&sourcepage=register
This presentation evaluates ASTM D7979-16 for the “direct” analysis of 30 PFCs and compares data to the solid-phase extraction EPA drinking water Method 537.
With increasing pressure of a higher sample throughput and fewer chemists, purification labs in medicinal chemistry groups need to be more productive now than ever before.
This presentation will describe a technique that allows the analyst to obtain a higher purity and better resolution using information from the preliminary analytical screening of these samples prior to purification.
This high performance liquid chromatograph (HPLC) is the first-ever instrument designed specifically for quantitative determination of cannabinoid content. Ready for use after one day of installation and testing, it provides a choice of three different HPLC methods and a dedicated user interface for a simplified workflow.
Change of Peptides and Free -Amino Acids Contents during Nanjing Dry-Cured Du...Agriculture Journal IJOEAR
— In order to explore the relationship between the change of peptides and free-amino acid (FAA) and its unique flavour, Dry-cured duck samples of different processing phases were used to study the change of free-amino acid by High Performance Liquid Chromatography (HPLC) in this paper, meanwhile the trichloroacetic acid precipitation method for modeling use to establish the quantitative predicated peptides. The changes of small peptides and free amino acids in the process were studied. The results showed that the level and amount of proteolysis increased with the processing time at traditional technology, meanwhile the amount of peptides were positively correlated with FAA contents (R 2 =0.86).
Determination of Insoluble Solids in Pretreated BiomassBiorefineryEPC™
Determination of Insoluble Solids in Pretreated Biomass
DISCLAIMER:
YOU AGREE TO INDEMNIFY BioRefineryEPC™ , AND ITS AFFILIATES, OFFICERS, AGENTS, AND EMPLOYEES AGAINST ANY CLAIM OR DEMAND, INCLUDING REASONABLE ATTORNEYS' FEES, RELATED TO YOUR USE, RELIANCE, OR ADOPTION OF THE DATA FOR ANY PURPOSE WHATSOEVER. THE DATA ARE PROVIDED BY BioRefineryEPC™ "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING BUT NOT LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE EXPRESSLY DISCLAIMED. IN NO EVENT SHALL BioRefineryEPC™ BE LIABLE FOR ANY SPECIAL, INDIRECT OR CONSEQUENTIAL DAMAGES OR ANY DAMAGES WHATSOEVER, INCLUDING BUT NOT LIMITED TO CLAIMS ASSOCIATED WITH THE LOSS OF DATA OR PROFITS, WHICH MAY RESULT FROM ANY ACTION IN CONTRACT, NEGLIGENCE OR OTHER TORTIOUS CLAIM THAT ARISES OUT OF OR IN CONNECTION WITH THE USE OR PERFORMANCE OF THE DATA.
NOVEL IPQCL AND FPQC TEST FOR OPTHALMIC PREPARATION AS PER IP, BP A...roshan telrandhe
Ophthalmic preparation are the sterile liquid or semisolid preparation meant to installation in to the eyes in the space between eye lids and eye ball .
These product must be sterile and are prepare under the same condition as that of parenteral preparation
The presence of Per- and Polyfluorinated Alkyl Substances (PFAS) in drinking water is being thoroughly studied due to the persistence of these compounds in the environment and their potential health effects. However, there is limited knowledge about the occurrence of these chemicals in bottled water, despite the increasing concerns about PFAS in the food supply. This poster shows results from a fast and simple direct injection method similar to draft EPA method 8237, using the Shimadzu triple quad LCMS-8050 to analyze seven commercially available samples of bottled water for 24 PFAS.
This is an Engg Biotechnology project based on medicinal plant i.e singapore cherry or jamaican cherry tree (scientific name Muntingia calabure ), we did in 2013 in GMIT college Davangere, karanataka, India. i have complete project detail what we did..,
IntroductionIn terms of total production tonnages used f.docxvrickens
Introduction
In terms of total production tonnages used for food, wheat is currently second to rice as the main human food crop and is the leading source of vegetable protein in human nutrition (Nutrient Data Laboratory). Aphids (Order Hemiptera) are major insect pests of world agriculture, damaging crops by removing photoassimilates and vectoring numerous plant viruses (Smith and Boyko, 2007). The grain aphid (Sitobion avenae) is considered a serious pest of commercial wheat in the UK. Many aphid species can develop resistance to insecticides (Devonshire and Field, 1991), and restrictions on the availability of active ingredients for insecticide production in Europe (European Directives 91/414/EEC) has prioritized research on crop varieties with resistance to aphid pests in UK agriculture (Painter, 1951; Panda and Kush, 1995; Smith, 2005). Most commercial wheat varieties have very little resistance to aphid pests (Lee, 1984; Dedryver and Di Pietro, 1984; Di Pietro and Dedryver, 1986; Migui, 2002; Migui and Lamb, 2003), with at best partial antibiosis, antixenosis and tolerancein some winter varieties (Lowe, 1984a; Lowe, 1984b; Havlícková, 1993). Wheat genetics are more complicated than that of most other crop species. Some wheat species are diploid, with two sets of chromosomes, but many are stable polyploids, with four sets of chromosomes (tetraploid) or six (hexaploid). Modern wheat varieties grown in the U.K. are hexaploid and have low genetic diversity for insect resistance traits (Ferry et al, 2011; Ogbonnaya et al, 2013; niab.com).
Diploid wheat (T. monococcum) lines have been observed to exhibit high levels of resistance against S. avenae (Migui and Lamb, 2003; 2004; Ferry et al, 2011). However, no previous studies have been carried out in these lines to investigate differentially expressed genes in response to aphid infestation. Therefore, differential proteomic analysis is being employed to identify putative defence responses in diploid wheat lines (Triticum monococcum L.) when subjected to grain aphid (S. avenae) feeding in order to better understand the basis of this observed resistance/tolerance.
In this lab you will conduct a 2D-electrophoresis separation of the wheat leaf proteome.
Methods
It is essential that you retrieve the full lab manual produced by GE healthcare for 2D electrophoresis:
Step 1 - Sample Solubilization
This has been done for you.
In this experiment 200mg of wheat leaf was ground in liquid nitrogen to a fine powder. The protein pellets available to you have been incubated in 10% (w/v) trichloroacetic acid/ acetone with 0.07% v/v 2-mercaptoethanol at -20 °C for 16 hours and then centrifuged at 35 000 x g for 20 mins.
> The pellets were washed with ice-cold acetone (0.07% 2-mercaptoethanol) 4-6 times and finally incubated at -20 °C for 1 h, and centrifuged at 12 000 x g for 15 min.
>>An acetone wash using 1 part sample: 3 parts acetone (w/v) was performed, the sample was vortexed to disperse pelle ...
LABORATORY MANUAL ON
QUALITY CONTROL OF ANIMAL FEEDS by Dr. G. DEVEGOWDA, PROFESSOR & HEAD, DEPARTMENT OF POULTRY SCIENCE
UNIVERSITY OF AGRI. SCIENCES
HEBBAL, BANGALORE
To avoid contamination, the aseptic technique is the method of reducing or removing contaminants from entering the operative field in surgery or medicine.
Hancock, MD, Oct. 17, 2016 - PetLife Pharmaceuticals, Inc. (OTC QB: PTLF) (the "Company"), a developer of a new generation of high potency veterinary cancer medications and Nutraceuticals for pets, has completed the corporate website overhaul as FDA/CVM submission and testing nears.
ESCOZINETM is an innovative polarized, potentiated bio-active peptide extracted from the Blue Caribbean Scorpion (Rhopularus Princeps) which contains amino acids, proteins and minerals. Medolife filed a patent in 2012 for its ESCOZINETM and polarization technology (Patent # US 8,097,284 B2). The polarization technique acts as a delivery system and additionally, amplifies the highly positive
This document is being provided by PetLife solely for the information of those persons to whom it is transmitted. No person in any jurisdiction may treat this document
as constituting either an offer to sell or solicitation of an offer to buy any securities in the Company. A prospective subscriber must rely solely on the terms of and
disclosure of information including important information regarding risks and conflicts of interest contained in the Company's final offering memorandum and related
documents, the only basis on which subscriptions may be made.
PetLife Pharmaceuticals, Inc. (PetLife Pharma) has developed and is launching a new generation of potentiated veterinary cancer medications and nutraceuticals.
PetLife Pharmaceuticals, Inc. (PetLife Pharma) has developed and is launching a new generation of potentiated veterinary cancer medications and nutraceuticals.
Has received an application/or a patentjor a new and us~ful invention. The title and description of the invention are enclosed. The requirements of law have been
complied with, and it has been determined that a patent on the invention shall be granted under the law.
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Taurus Zodiac Sign_ Personality Traits and Sign Dates.pptxmy Pandit
Explore the world of the Taurus zodiac sign. Learn about their stability, determination, and appreciation for beauty. Discover how Taureans' grounded nature and hardworking mindset define their unique personality.
Cracking the Workplace Discipline Code Main.pptxWorkforce Group
Cultivating and maintaining discipline within teams is a critical differentiator for successful organisations.
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Enterprise Excellence is Inclusive Excellence.pdfKaiNexus
Enterprise excellence and inclusive excellence are closely linked, and real-world challenges have shown that both are essential to the success of any organization. To achieve enterprise excellence, organizations must focus on improving their operations and processes while creating an inclusive environment that engages everyone. In this interactive session, the facilitator will highlight commonly established business practices and how they limit our ability to engage everyone every day. More importantly, though, participants will likely gain increased awareness of what we can do differently to maximize enterprise excellence through deliberate inclusion.
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Who might benefit? Anyone and everyone leading folks from the shop floor to top floor.
Dr. William Harvey is a seasoned Operations Leader with extensive experience in chemical processing, manufacturing, and operations management. At Michelman, he currently oversees multiple sites, leading teams in strategic planning and coaching/practicing continuous improvement. William is set to start his eighth year of teaching at the University of Cincinnati where he teaches marketing, finance, and management. William holds various certifications in change management, quality, leadership, operational excellence, team building, and DiSC, among others.
As a business owner in Delaware, staying on top of your tax obligations is paramount, especially with the annual deadline for Delaware Franchise Tax looming on March 1. One such obligation is the annual Delaware Franchise Tax, which serves as a crucial requirement for maintaining your company’s legal standing within the state. While the prospect of handling tax matters may seem daunting, rest assured that the process can be straightforward with the right guidance. In this comprehensive guide, we’ll walk you through the steps of filing your Delaware Franchise Tax and provide insights to help you navigate the process effectively.
The world of search engine optimization (SEO) is buzzing with discussions after Google confirmed that around 2,500 leaked internal documents related to its Search feature are indeed authentic. The revelation has sparked significant concerns within the SEO community. The leaked documents were initially reported by SEO experts Rand Fishkin and Mike King, igniting widespread analysis and discourse. For More Info:- https://news.arihantwebtech.com/search-disrupted-googles-leaked-documents-rock-the-seo-world/
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It is crucial for the taxpayers to understand about the TDS Return Filing Due Date, so that they can fulfill your TDS obligations efficiently. Taxpayers can avoid penalties by sticking to the deadlines and by accurate filing of TDS. Timely filing of TDS will make sure about the availability of tax credits. You can also seek the professional guidance of experts like Legal Pillers for timely filing of the TDS Return.
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Sustainability has become an increasingly critical topic as the world recognizes the need to protect our planet and its resources for future generations. Sustainability means meeting our current needs without compromising the ability of future generations to meet theirs. It involves long-term planning and consideration of the consequences of our actions. The goal is to create strategies that ensure the long-term viability of People, Planet, and Profit.
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1. Introduction and Key Concepts of Sustainability
2. Principles and Practices of Sustainability
3. Measures and Reporting in Sustainability
4. Sustainability Implementation & Best Practices
To download the complete presentation, visit: https://www.oeconsulting.com.sg/training-presentations
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2. PETLIFE- White Paper
Stages of Production 1
2014
Stages of Production of Escozine For Pets™
Table of Contents
I. Collection of Scorpions from the Scorpion Reservation......................................................2
II. Extraction of Venom, Purification and Therapeutic Dose Preparation .............................4
III. Polarization of Extract and Quality Control of Polarization..................................................9
IV. Manufacturing, Quality Control, Warehouse And Shipment..............................................12
3. PETLIFE- White Paper
Stages of Production 2
2014
Production Procedures
Escozine for Pets™ has 4 major production steps.
1. Collection of Scorpions from the Scorpion Reservation.
2. Extraction of venom, purification and therapeutic dose preparation.
3. Polarization of extract and quality control of Polarization
4. Manufacturing, quality control, warehouse and shipment.
I. Collection of Scorpions from the Scorpion Reservation
Dominican blue scorpions are from the Buthidae family: Rhopalurus Princeps.
They are collected from Medolife Corp’s 50,000m2
Scorpion Reservation, located in
the South of the Dominican Republic.
The reservation is divided in to SECTORS with numeric identification. Scorpions are
carefully collected every 22 days for venom extraction. These SECTORS are kept
separated from one another, but all scorpion populations are given the same care and
attention by Medolife veterinarians and biologists. The consistency in diet, breeding,
timing of extraction and population control is intended to support consistency of venom
concentration per each extraction.
Adult and young adult scorpions are chosen for the derived peptide extraction.
Scorpions collected for venom extraction are placed in small individual plastic
containers with individual numeric identification that specify the SECTOR they inhabit.
The plastic containers have holes on the top so that the scorpions have enough oxygen
during delivery to laboratory for extraction.
This numeric identification matches a collection calendar and thus prevents multiple
extractions of venom from same scorpions within a 22-day interval.
5. PETLIFE- White Paper
Stages of Production 4
2014
II. Extraction of Venom, Purification and Therapeutic Dose Preparation
Scorpions are collected for extraction in individual plastic containers and then delivered to the
laboratory where the pre-production process begins. Extraction: The derived peptide solution
from the Rhopalurus Princeps is extracted using the well-known technique of electric inductance.
The drops are calibrated using a five decimal analytical balance: the results produce an average
of 6.6 ± 1.1 µg per drop. A few drops of distilled water are used to make the derived peptide
flow through the collection test tube.
A 100 ml glass container filled with 100 ml of medical distilled water is used for extraction.
After using electrical stimulation, the drops of scorpion venom are captured in this single 100 ml
sterilized glass container.
Centrifugation: The total amount of derived peptide is centrifuged for 15 minutes at a velocity of
6,000 rpm. The lower solid portion is then separated by decantation.
Filtration: Scorpion venom is a complex mixture of salts, small molecules, peptides, and
proteins. The laboratory filters the venom using Glass Fiber Membrane Filters, 0.80µm, 25mm,
1pk/50pcs to ensure the sterilization and purification.
Therapeutic Dose Preparation: The spectrophotometric analysis is done within a UV range using
a Beckman uv-vis spectrophotometer. The absorbance reading is approximately 278 nm. An
average of 15% of the peptide solution remains in the solid layer.
Different dilutions of the derived peptide solution have similar relative absorbance at 278 nm.
The quantity used for the final product is 0.0035 µg per 120 ml.
The dilution of the concentration for Escozine for Pets™ has to be calculated from the portion of
the amount of drops used in the initial extraction from scorpions assuming a 15% loss due to
centrifugation. The absorbance of the initial solution is taken as a parameter to extrapolate the
final dilution.
7. PETLIFE- White Paper
Stages of Production 6
2014
Lowry's method for determination of protein concentration
To determine the most therapeutically effective dose concentration of Escozine for
Pets™ we use Lowry’s Method.
The Lowry Method (1951) is a colorimetric method of quantitative evaluation of the
proteins. The reagent forms a colored complex with the proteins when added to the
sample; the intensity of color of the resultant solution in proportion to the protein
concentration is measured according to the Lambert-Beer law.
1. MATERIAL AND REAGENTS
a. MATERIALS:
1. Test-tubes.
2. Pipettes.
3. Colorimeter (Beckman)
b. REAGENTS
1. Reagent A: Na2CO3 to 2 %, NaOH 0.1 M
2. Reagent B1: CuSO4 × 5H2O to 1%
3. Reagent B2: sodic-potassic tartrate to 2%
4. Reagent C: It is prepared at the moment of initiating the essay,
mixing A, B1 and B2 in proportions 50:0, 5:0.5 (in volume)
5. Reagent Folin-Ciocalteau: commercial reagent diluted to 1/4
6. Pattern Solution of albumin of bovine whey (2 mg/ml)
2. EXPERIMENTAL PROCEDURE
A pattern curve is drafted, using different volumes of a solution of albumin of
bovine whey (2 mg/ml). The concentrations that the samples have are
determined by interpolation of the absorption values in the curve pattern. The
pipette 0, which only contains distilled water and the reagents, serves as the
target for the adjustment of the colorimeter to zero absorption.
Error definition Water
Pattern
(2mg/ml)
Target React. C Folin dil. Abs.580nm
0 1.0 ml -- -- 5 ml 0.5 ml
1 0.9 ml 0.1 ml -- 5 ml 0.5 ml
2 0.8 ml 0.2 ml -- 5 ml 0.5 ml
3 0.7 ml 0.3 ml -- 5 ml 0.5 ml
4 0.6 ml 0.4 ml -- 5 ml 0.5 ml
5 0.7 ml -- 0.3 ml 5 ml 0.5 ml
6 0.5 ml -- 0.5 ml 5 ml 0.5 ml
Steps to be followed:
8. PETLIFE- White Paper
Stages of Production 7
2014
1. Number from 0 to 6, 10 ml plastic pipettes.
2. Pipette the water quantities, pattern solution of albumin and target solution indicated
in the chart.
3. Prepare the reagent C, from A, B1 and B2.
4. Pipette reagent C to all the pipettes. Mix the content of every pipette and allow it to
rest 15 minutes in darkness.
5. Next, add the reagent of Folin (diluted ¼) to all pipettes, mixing well. Allow to rest 30
minutes in darkness so that the colored reaction develops completely.
6. Read absorptions in the colorimeter to 580 nm. Previously the device is calibrated to
A=0 with the target (pipette nº 0); only the color produced by the proteins is
measured (the color is reduced due to the reagents).
TREATMENT AND DISCUSSION OF THE RESULTS
Obtain the calibration curve representing the absorption levels of the pipettes 0 to 4
opposite to the concentration (or quantity) of protein in every pipette, which is calculated
previously from the information in the chart.
Determine the protein concentration of the target sample, expressing the result in mg/ml
(allow the realized dilutions in the calculation).
Estimation of the protein concentration (Direct reading of the venom in the
spectrophotocolorimeter)
The protein content is estimated from the measurement of the absorption to 280 nm in a
1cm vat, assuming an absorption of 1 mg/ml of raw venom.
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λ Reading Lamp
PROG RS
ABS % T
UV VIS
STEP BSTP REA XY + 7 8 9
RCL STOP CALL ↓ - 4 5 6
λ CH X 1 2 3
CALL GOTO
SCA
N
← % 0 . Entr
FUNC ALFA
OPERATING GRADIENT TABLE #: 6
Time Flow % A % B % C % D Curve
Inicial 0 100 *
2.00 min 0 100 6
12.00
min
0 100 6
14.00
min
100 0 6
44.00
min
100 0 6
46.00
min
100 0 6
←
↑
→HOME
↓
1 2 3
4 5 6
7 8 9
CLEAR 0 .
ENTER
Refrigeration:
Once the therapeutic dose is determined and the extraction concentrate is ready for
manufacturing, the container is sealed and placed in a refrigerated environment. The
container is kept in that refrigerated environment for thirty minutes until the venom
reaches a maximum of 12C (52F). At this time, the container will be packed into a
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refrigerated compartment that will keep an even temperature range between 12 and
14C (52-58F).
III. Polarization of Extract and Quality Control of Polarization
Dose preparation before polarization:
All measurement flasks and glass tanks are sanitized in advance.
Upon receiving the purified concentrated extract, the physicist-chemist from the
polarization department measures the required amount of medical grade distilled water
for additional dilution. This dilution is not a final dilution but only for the polarization
process.
Polarimeter test for non-polarized extract: The laboratory
technician separates a small amount of extract and runs
Polarimeter test (Automatic Polarimeter Rudolph (RRA))
This test identifies the atomic movement of the extract (levorotatory or dextrorotatory
movement, and the angular rotation of the atoms as well as the molecular frequency of
the extract. The data is used to compare the molecular activity of the extract before and
after polarization to control and measure the required polarization level for therapeutic
effect.
Polarization: The laboratory technician under the supervision of physicist-chemist then
pours the diluted extract into polarizer’s glass tank. The physicist-chemist then uses the
formula to calculate the speed of the polarizer’s pump and the required quantity of
cycles that is necessary to run the extract through electromagnetic field in order to
reach the required amount of polarization. He then adjusts the power (strength) of
electromagnets, frequency and time on the frequency generator; when all preparation is
completed, the technician starts the polarization process.
Upon completion of polarization, the physicist–chemist checks the polarization level with
an additional polarimeter test.
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ESCOZINE POLARIZATION: PRE-PRODUCTION / POSTPRODUCTION CONTROL
TEST
Blue Line: Spectrum of
the environment
Violet Line: Spectrum of
Non Polarized Escozine.
Red Line: Spectrum of
Polarized Escozine
Blue Line: Spectrum of Laboratory Environment
Violet Line: The spectrum of the Escozine for Pets™ with the mountain (marked by a
cycle) is in the region 3500-3000 cm¯¹ wave number.
Red Line: The spectrum of the polarized ESCOZINE FOR PETS™. The mountain is
not present and the spectrum is in the region 2000-500 cm¯ˉ¹. The wave number is
more defined due to the increase of absorbance and the increase of polarization
levorotatory isomer.
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This control test compares the Escozine for Pets™ condition before and after the
polarization. When therapeutic level of polarization is achieved, the extract is sent to the
production facility for the final manufacturing process.
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IV. Manufacturing, Quality Control, Warehouse And Shipment
Receiving: The manufacturing facility of the pharmaceutical laboratory receives purified
and concentrated venom. This concentrate is received in individual containers ranging
in size from 100ml to 0.5 liters.
Manufacturing: The dosimeter of automated filling machinery is calibrated to inject 120
ml of mixture into the sterilized bottles. The correct concentration is determined by the
biochemist depending on the production capacity and order requirements. The
concentrate of scorpion extract is mixed with an excipient of polarized medical-grade
distilled water in a container; the mixing supervisor then monitors every dosimeter
injection of the mixture into the bottles. This procedure disperses the concentrate with
recipient evenly. After the 120 ml bottles are filled with the mixture, bottle caps are
screwed and sealed with plastic shrink wrap neck bands.
In the next step the bottled and sealed product moves by conveyer belt to the labeling
section where the expiration date and lot number are printed onto the labels. Following
this procedure, the bottles are packed in individual cartons with instructions and a 20-30
ml plastic cup and a 1ml plastic dropper. Next is the packaging process where individual
cartons get inserted into transportation boxes. The bulk version of the product is
transferred into a sealed, refrigerated storage room.
Storage/Refrigeration: The bulk container is stored in a dark, refrigerated environment
at the storage facility which is maintained at a temperature between 12 and 14 C (52F
and 58F) while awaiting shipment.
Quality Control: Individual containers of the final product from each and every batch
are collected for quality control. Microbiological tests and other additional tests are
performed to insure the right concentration of the main ingredient is in each batch. The
therapeutic concentration level is tested by using mass spectrometry analyses; the
results are compared to standardized mass spectrometry readings for the product. No
product is shipped until the batch has undergone testing.
Batch Rejection:
A batch of final product is rejected upon the following conditions:
1. Microbiological Test: If one bottle of final product tests positive for bacterial
contamination, then entire batch is rejected.
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2. Incorrect concentration: If more than 3 bottles of the final product show ± 0.05%
difference from the standardized therapeutic concentration by mass spectrometry
analyses, and then the entire batch is rejected.
Shipping: PetLife employees supervise the shipment and handling of all orders to
ensure that the packaging and boxing meets the highest standards.
○○○ DELIVERY TO CUSTOMERS ○○○
PETLIFE™- White Paper