13. Y2H Yeast two hybrid system
Yeast two-hybrid is based on the reconstitution of a
functional transcription factor (TF) when two proteins
or polypeptides of interest interact. This takes place
in genetically modified yeast strains, in which the
transcription of a reporter gene leads to a specific
phenotype, usually growth on a selective medium or
change in the color of the yeast colonies. The most
popular reporter genes are HIS3 to select yeast on a
medium lacking histidine, and LacZ to screen yeast
in a colorimetric assay