project work

1. HEPATITIS-B RAPID CARD TEST,
HCV RAPID CARD TEST,
MALARIA Pf/Pv Ag RAPID TEST

2. INDEX
• Hepatitis-b rapid card test
• HCV Rapid card test
• Malaria Pf/Pv Ag Rapid Test

3. HEPATITIS-B RAPID CARD TEST
The Hepatitis-B test uses for
the qualitative detection of
HBsAg in serum or plasma.

4. PROCEDURE :
 Remove the testing device from the foil pouch by
tearing at the ‘notch’ and place it on a levelled
surface .
 Holding the sample dropper vertically, add 2
drops of the specimen into the sample well
marked ‘S’ .
 Read results within 20 minutes .

5. RESULTS :
POSITIVE TEST

6. HCV RAPID CARD TEST
HCV Rapid Card test is
a rapid test to
qualitatively detect the
presence of antibody
to HCV in a human
serum or plasma.

7. PROCEDURE :
Remove the test cassette from the sealed pouch & Place the test
cassette on a clean and level surface.
Hold the dropper vertically and transfer 1 drop of serum or plasma
(25 μL) to the specimen well of the test cassette .
 then add 2 drops of buffer (80 μL) and start the timer. Avoid
trapping air bubbles in the specimen well.
Wait for the colored line(s) to appear. The test result should be read
at 10 minutes. Do not interpret the result after 20 minutes.

8. RESULTS :
POSITIVE
TEST
NEGATIVE TEST

9. Malaria Pf/Pv Ag Rapid Test
The Malaria Pf/Pv Ag Rapid Test
is used for the detection of
Plasmodium falciparum(Pf) &
vivax(Pv) antigen in human blood
specimen.

10. Procedure :
 open the pouch at the notch and remove device. Place the test
device on a clean, flat surface.
 Add 5 μL of blood specimen into the sample well making sure that
there are no air bubbles.
 Then add 2 drops (about 50-100 μL) of lysis Buffer immediately into
the buffer well.
 Set up timer and result can be read at 30 minutes. It may take
more than 20 minutes to have the background become clearer.

11. RESULTS :
NEGATIVE RESULT

12. Pv positive Pf positive Pv & Pf positive
POSITIVE RESULT
INVALID

13. RHELAX-SLE TEST FOR ANTI-DNP
RHELAX-SLE is a latex
agglutination slide test for
Determination of anti-DNP
antibodies associated
with Systemic Lupus
Erythematosis

14. PROCEDURE :
Place one drop (30 μL) of sample onto one of the reaction circles
of the slide using pipette.
Place one drop of positive & negative control onto separate
reaction circles of the slide .
Add 1 drop of SLE-Latex Reagent (40 μL) to each sample and
control taken on the slide .
Mix with separate mixing sticks, spreading the mixture uniformly
over the entire reaction circle .
Immediately start a stopwatch for three minutes .Observe under a
suitable light source for any degree of agglutination .

15. RESULTS :
 Agglutination is a
positive test result and
indicates presence of Anti-
DNP in the test specimen
 No agglutination is
negative test result and
indicates absence of Anti-
DNP in the test specimen

16. THANK YOU