Organic Name Reactions for the students and aspirants of Chemistry12th.pptx
Sterelization 08 04-2021
1.
2. Sterilization –Anintroduction
Importance of sterilization
Methods
Advantages & Disadvantages
Effects of Sterilization
Conclusion and future prospects
3. The process of killing or
removing bacteria and all other
forms of living micro-organisms
and there spares from preparation.
Essential concept in the
preparation of sterile
pharmaceutical products
Its aim
is to provide a product that is safe and eliminates the
possibility of introducing microorganisms
4. Medical Sterilization
Prevents the Growth of Diseases
In any medical tool/device used, bacteria comes onto it. If left unchecked
or not disinfected properly, it is highly likely that bacteria willgrow.
Prevents the Spread of Diseases
If surgical equipment is not properly sterilized, patients treated are
exposed to a disease the previous patient had.
Prevents Double Surgeries
If unsterilized equipment is used, it can cause an infection leadingto
another surgery later on in order to remove it.
This is costly and can cause many life-threatening complications.
Importance of sterilization
5.
6.
7. THREE METHOD :-
1. Physical method
a)Sterilization by filtration
b)Sterilization by radiation (gamma radiation)
2. Chemical method
a) Gaseous sterilization
b) Sterilization by disinfectant
3. Mechanical method
Pass through bacteria-proof filter
10. Mechanism of action:
Instrument- ‘HOT AIR
OVEN’
specially designed instrument - electrically heated and
thermostatically controlled.
Expose at 160 ºC for 1 hour.
Advantage-
it is suitable method for sterilization of
substances destroyed by moisture.
Disadvantage-
long heating time, high temperature, effect on materials
Applications-
For powders, containers and equipments
11. Effectiveness-
Mechanism of action-
Instrument- ‘AUTOCLAVE’
Heating process in autoclave - saturated steam under pressure is allowed to
penetrated through materials for 15 minutes and temperature 121º c.
Wrapping materials-
Advantage-
micro organism are killed most efficiency in lesser time
due to high pressured saturated steam
Disadvantage-
unsuitable for materials not withstanding
temperature of 115ºC or more during heating
Applications-
12.
13. Two techniques involved:
Alteration of chemicals lead to form new compound in
cells destroying the micro-organism itself
Vital structure like nuclear protein are destroyed killing the
micro-organism.
e.g., Co-60 - used for gamma ray sterilization process.
Gamma rays –
generally obtained from radio isotope(Co-60)during
disintegration of unstable atoms
kill micro-organisms by isolating atoms of essential substanceof
cells present in them.
14.
15. 1. No significance rise in temperature
2. Continuous process due to short exposure
time.
DISADVANTAGE
1. May lead to color change.
2.Solubility of preparation leading to
decomposition of certain materials.
16. Absolute process
Mechansim of action
Liquid flow through a filter
Types of filters
Aseptic processing
Applications
17.
18.
19. Gaseous sterilization-
Ethylene oxide used.
Special type of chemical sterilization using gases and vapour
The gas used is safe & non-inflammable.
Now-a-days, ethylene oxide most widely used gaseous
sterilization agent in medical science.
20. 1. It has penetration power quite useful for sterilizing
surgical instruments (such as catheter, needles, plastics,
disposables)
Disadvantages:
1. Very slow sterilization process
2. Very costly equipment
21. Decontamination - removal of microorganisms contaminating
an object
Preservation - preventing methods of microbes-caused
spoilage of susceptible products(pharmaceuticals, foods)
Sanitization - removal of microbes that pose a threat to the
public health, food industry, water conditioning
sanitizer-an agent, usually a detergent, that reduces the numbers
of bacteria to a safe level
22. Chemical agents
Alcohols, aldehydes, halogen, phenols, surfactants,
heavy metals
e.g., ethylene oxide – most commonly used for sterilisation
Advantages:
1. Widely used in hospitals for materials that cannot
withstand steam sterilisation
Disadvantages
1. 40-60% humidity in sterilizing chamber
23. The solution to be sterilized is passed through depth-
filter or screen-filter which includes
Particulate filters
Microbial filters
Final filter
Pharmaceutical solutions are sterilized by this method.
The micro-organism are physically removed by
absorption on the filter medium or by mechanism.
Filtration filling and sealing processes are under a septic
condition.
Sterilization test must be done.
24. There are 3 effects:
1.Gamma radiation sterilization of medical device is
common but irradiation effect at 2.5 mega rad on a bone
replacement material when started and modified property
when investigated by Creep test.
2.Irradiation increase Creep resistance of material with
the formation of crosslink and then increase in crystality
respectively.
3.Biodegradable materials sterilized by gamma radiation
may be associated with some advance effect.
25. In the near future the main challenge asbiomedical
engineer is to make the sterilization process
More reliable
Reusable
Non-polluting
Costeffective
Lesstime consumingand
Better availability
at hospitals, pharmaceutical company, drug industry and
food processingplants.