The document details the HIV Tri Dot Test, a rapid serological test that detects HIV-1 and HIV-2 antibodies in patient samples. The test involves a procedure where serum is applied to regions with specific antigens, and color changes indicate positive, negative, or invalid results. The interpretation of results is based on the presence of color dots in specified regions of the test kit.

1. SWASTHYA KALYAN COLLEGE
OF PARAMEDICAL
TECHNOLOGY
HIV TRI DOT TEST
RAPID SEROLOGICAL TEST
presented by ..............................................PANKAJSAINI

2. CONTENT:-
• INTRODUCTION
• PRINCIPLE
• PROCEDURE
• INTERPRETATION

3. INTRODUCTION:-
• The HIV TRI DOT kit consist of three different regions.
(I). HIV-1 REGION:-
This region consist of precauted HIV-1 virus antigen such as gp41 & gp120 on
immuno filtration membrane.
(II). HIV-2 REGION:-
This region consist of precauted HIV-2 virus antigen such as gp36 on immuno
filtration membrane.
(III). CONTROL REGION:-
This region consist of precauted anti-mouse antibodie on immuno filtration
membrane.
(IV). CONJUGATE SYSTEM:-
. In HIV tri dot test, conjugate system is used known as a protein ‘A’ conjugate.
. This system is consist of anti HIV antibody with conjugate.

4. PRINCIPLE:-
• AT HIV-1 REGION:-
• If the patient suffer from AIDS by HIV-1 virus, the patient will have
HIV antibodies(gp120 & gp41) in the serum sample.
• After application of sample, these HIV antibodies will bind with
precauted antigens in HIV-1 region.
• This binding lead to the formation of Ag-ab complex.
• The conjugate system consist of anti-Hiv antibody with conjugate
after application of conjugate system this will bind with perform Ag-
ab complex.
• This binding activate the conjugate system and produce colour.
• Production of colour dot indicate the positive result for the presence
of HIV antibody against Hiv-1 virus in the patient sample.

5. AT ‘C’ REGION:-
• The conjugate system consist of anti HIV antibody which act as a
mouse antibody.
• This mouse antibody act as antigen for anti mouse antibody which is
already precauted on ‘C‘ region.
• The binding between mouse antibody & anti mouse antibody
activate the conjugate system & produce a colour.
• Production of colour dot at ‘C‘ region indicate that the procedure is
correct.

6. PROCEDURE
• Take a HIV tri dot kit.
• Open a foil pouch.
• Lable the kit with patient name & patient id.
• Add three drops of buffer solution.
• Add one drop of patient sample.
• Add five drops of buffer solution.
• Add two drops protein ‘A‘ conjugate system.
• Add five drops of buffer solution.
• Wait for the result.

7. INTERPRETATION
• POSITIVE RESULT:-
• +ve result for Hiv-1 virus. +ve result for Hiv-2 virus
Two colour dot appears Two colour dot appears
(I). One colour dot at (I). One colour dot at Hiv-2
Hiv-1 region. Region.
(II). Second colour dot at (II). Second colour dot
‘C’ region. At ‘C’ Region.

8. • NEGATIVE RESULT:-
Only one colour dot appear at ‘C’ region.
INVALID RESULT:-
When there is no colour dot at ‘C’ region.