estimation of creatinine and uric acid

1. Biochemistry Practical 1
(Estimation of Creatinine & Uric acid)
By : Osama Al-Zahrani
@OSAMA_Z96
*‫مالحظة‬:‫الرجوع‬ ‫عن‬ ‫يغني‬ ‫ال‬ ‫العمل‬ ‫هذا‬‫لل‬‫ـ‬(Handout)‫المرفق‬

2. - A colorimeter is a device that measures the absorbance of particular
wavelengths of light by a specific solution.
- This device is commonly used to determine the concentration of a known
solute in a given solution
Colorimeter

3. Uric acid
Estimation

4. Procedure :
Uric acid

5. Equation:
Uric acid conc. (mg/dl) = × conc. Standard
Example :
If the Absorption test (Ab.T) = 0.257 ml
If the Absorption standard (Ab.St) = 0.243 ml
If the conc. Standard = 8 mg/dl
Then : Uric acid conc. = × 8 = 8.46 mg/dl (Hyperuricemia for male & female).
Normal value of Uric acid in human plasma :
Male : 3.4 – 7 mg/dl
Female : 2.4 – 6 mg/dl
Uric acid
Ab.S
Ab.T
0.243
0.257

6. If the result was at Normal value, the interpretation will be :
This patient has Normal Level of uric acid.
If the result was Higher than normal value, the interpretation will be :
This patient has Hyperuricemia
If the result was Lower than normal value, the interpretation will be :
This patient has Hypouricemia
Interpretation of the result

7. Clinical significance

8. Creatinine
Estimation

9. Creatine :
Creatine is a nitrogenous organic acid, synthesized
primarily in the liver,
then transported to the muscle and converted to
creatine phosphate, where it serves as high-energy
source
Creatinine :
Creatinine is a chemical waste molecule that is
generated from muscle metabolism, and it is
produced from creatine.
Reference ranges :
Male : 0.9 – 1.3 mg/dl
Female : 0.6 – 1.1 mg/dl
Creatine & Creatinine

10. Clinical correlation of Creatinine
Creatinine concentration Measurement :
Is used to determine the sufficiency of kidney function & severity of kidney damages.
Creatinine clearance test :
Is a test that compares the creatinine level in urine with its level in blood,
and it is used to assess the renal function.