Our core business is the study of pharmacokinetics in the human brain at early preclinical stages using accurate predictive models and recent tools developed from human brain tissues. These tools include a proprietary blood-brain barrier model and fresh human brain slices that preserve physiological functions. We also have a physiologically-based pharmacokinetic model called Brain Exposure Prediction that provides in vivo time-curves of drug concentration in the brain based on in vitro generated pharmacokinetic parameters. BrainPlotting supports preclinical development plans and screening through these models and tools.

1. Contact : nicolas.perriere@brainplo2ng.com
Human data
from preclinical stages
To that end, we have developed in vitro and ex vivo tools from fresh
Human brain tissues, such as a Blood-Brain Barrier (BBB) proprietary
model, and fresh Human brain slices which preserve physiological
functional properties.
Our PBPK (Physiologically Based PK) model, Brain Exposure Prediction
model (BEP), provides also in vivo time-curves of drug free
concentration in the brain based on in vitro generated PK brain
parameters.
BrainPlotting is a CRO for screening purposes or for custom R&D
BrainPlotting supports you in designing preclinical development plans for your drug
Brain Exposure Prediction
Our core business is the study of pharmacokinetics (PK) in the Human brain at early
preclinical stage by using the most accurate predictive models and most recent tools.
Endocytosis
or Exocytosis
Facilitated
transportEfflux
Simple
diffusion
Paracellular
pathway
Tight
junctions
Blood Stream
Brain Parenchyma
Brain Extracellular Fluid
(ECF)
Glial & Neuronal cells
Brain Intracellular Fluid (ICF)
BBB
To reach its target, the molecule of interest has to cross the BBB and enter the extra-cellular
(ECF) or intra-cellular fluids (ICF) of neural cells through specific transportation mechanisms
fu,plasma
fu,ecf
fu,icf
Measures of Permeabilities through the BBB
Measure of permeability coefficients
(Pe) IN & OUT, and efflux ratio (ER).
Characterization of tight junctions and
transporters expression
Evaluation of functional properties as
Paracellular & Transcellular pathways,
ABC- and SLC- transporters
BLOOD: A
BRAIN: B
in vitro BBB model
Determination of Unbound fractions (fu)
Study of the behavior of the
molecule in plasma, brain homo-
genate and fresh brain slices:
- Measures of fu plasma & fu brain
- Fu determination in brain Intra &
Extra Cellular Fluids
ECF surrounding brain cells
Prediction of unbound concentration of your compound in the Human brain
parenchyma. To that end we developed a PBPK model based on in vivo PK,
Human data and in vitro specific informations
Brain/Plasma
Ratio
defined for
your product
Time
www.brainplo2ng.com