Morphology and biochemical tests for Pseudomonas syringae pv lachrymans

1. BACTERIOLOGY
PRACTICALS
ANGULAR LEAFSPOT OF CUCUMBER
- Pseudomonas syringae pv. lachrymans

2. University of Agricultural Sciences, GKVK, Bengaluru
Department of Plant Pathology
PRESENTED BY:
MADHU.J
PAMB 1321
Jr.Msc plant pathology
PRESENTED TO:
DR.M.K.PRASANNA KUMAR SIR
MR.B.S.CHANDRASHEKAR SIR
Dept of PLANT PATHOLOGY
COLLEGE OF AGRICULTURE,GKVK, BANGLORE-560 065
PAT 503 PLANT BACTERIOLOGY (2+1)
ANGULAR LEAFSPOT OF CUCUMBER
- Pseudomonas syringae pv. lachrymans

3. SYMPTOMS IDENTIFICATION
The leaf spot is irregularly shaped with a water-soaked appearance; bounded by
the leaf veins the shapes of spots are angular.
Cheemanahalli Village
Chinthamani Taluk
Chikkaballapura District

4. PREPARATION FOR AUTOCLAVE

5. ISOLATION OF BACTERIA
Lesions

6. ISOLATION OF BACTERIA
Growth of Pseudomonas syringae pv. Lachrymans on nutrient agar medium
Observations are made for development of well seperated,typical colonies in plate

7. SUBCULTURE
Select single well seperated colony from plate then streak on plate containig fresh
nutrient agar

8. COLONY MORPHOLOGY
Bacterium was identified
as Pseudomonas syringae based on
creamy white color colonies on
the nutrient agar plate

9. MAINTENTENANCE OF PURE CULTURE
Select single well seperated colony from plate then streak on plate containig fresh
nutrient agar maintaing pure culture
single well seperated colony from plate then streak on slant containig fresh nutrient agar

10. PREPARATION OF CULTURE BROTH
single well seperated colony from plate inoculated to nutrient broth
Preparation of nutrient broth for bacteria

11. PROVING OF PATHOGENICITY
Healthy seedlings were Raised in glass house
Plants are injected and sprayed with 48 hr old bacterial culture and control is also
maintained
Plant pathology glass house,GKVK

12. SYMPTOMS DEVELOPMENT
Observe for typical symptoms of the disease - angular spots on leaves

13. BIOCHEMICAL TESTS

14. GRAM STAINING

15. STARCH HYDROLYSIS TEST

16. PEROXIDASE TEST

17. SIMMON CITRATE TEST

18. LEAVENS TEST

19. KOH TEST

20. CELLULOBIOSE TEST

21. GELATIN TEST

22. YEAST CALCIUM CARBONATE DEXTROSE
AGAR (YCDA) TEST