Writing a good case study requires that you follow a particular case study format. A good case study format will help you to uncover person’s or business’s particular problem and how it was solved
Work on your strengths for the gate exammeghasingh812
The Graduate Aptitude Test in Engineering (GATE) essentially examines the aptitude of engineering graduates/students with simple problems and applying engineering concepts to solve them. So, GATE aspirants have to develop the ability to solveconceptual and practical problems.
Hi,Please, do not use Mayo clinic or internet source as referencSusanaFurman449
Hi,
Please, do not use Mayo clinic or internet source as reference. Please, use peered reviewed articles and/or Textbooks.
Thanks a lot!
Discussion: Assessing Musculoskeletal Pain
The body is constantly sending signals about its health. One of the most easily recognized signals is pain. Musculoskeletal conditions comprise one of the leading causes of severe long-term pain in patients. The musculoskeletal system is an elaborate system of interconnected levers that provides the body with support and mobility. Because of the interconnectedness of the musculoskeletal system, identifying the causes of pain can be challenging. Accurately interpreting the cause of musculoskeletal pain requires an assessment process informed by patient history and physical exams.
In this Discussion, you will consider case studies that describe abnormal findings in patients seen in a clinical setting.
To prepare:
· Your Discussion post should be in the Episodic/Focused SOAP Note format rather than the traditional narrative style Discussion posting format. Refer to Chapter 2 of the Sullivan text and the Episodic/Focused SOAP Template in the Week 5 Learning Resources for guidance. Remember that all Episodic/Focused SOAP notes have specific data included in every patient case.
· Review the following case studies:
With regard to the case study, you were assigned:
· Review this week's Learning Resources and consider the insights they provide about the case study.
· Consider what history would be necessary to collect from the patient in the case study you were assigned.
· Consider what physical exams and diagnostic tests would be appropriate to gather more information about the patient's condition. How would the results be used to make a diagnosis?
· Identify at least five possible conditions that may be considered in a differential diagnosis for the patient.
Note: Before you submit your initial post, replace the subject line ("Discussion - Week 8") with "Review of Case Study ___." Fill in the blank with the number of the case study you were assigned.
By Day 3 of Week 8
Post an episodic/focused note about the patient in the case study to which you were assigned using the episodic/focused note template provided in the Week 5 resources. Provide evidence from the literature to support diagnostic tests that would be appropriate for each case. List five different possible conditions for the patient's differential diagnosis, and justify why you selected each.
Note: For this Discussion, you are required to complete your initial post before you will be able to view and respond to your colleagues’ postings. Begin by clicking on the "Post to Discussion Question" link, and then select "Create Thread" to complete your initial post. Remember, once you click on Submit, you cannot delete or edit your own posts, and you cannot post anonymously. Please check your post carefully before clicking on Submit!
The Case study: Knee Pain
Photo Credit: University of Virginia. (n.d.). N ...
Writing a good case study requires that you follow a particular case study format. A good case study format will help you to uncover person’s or business’s particular problem and how it was solved
Work on your strengths for the gate exammeghasingh812
The Graduate Aptitude Test in Engineering (GATE) essentially examines the aptitude of engineering graduates/students with simple problems and applying engineering concepts to solve them. So, GATE aspirants have to develop the ability to solveconceptual and practical problems.
Hi,Please, do not use Mayo clinic or internet source as referencSusanaFurman449
Hi,
Please, do not use Mayo clinic or internet source as reference. Please, use peered reviewed articles and/or Textbooks.
Thanks a lot!
Discussion: Assessing Musculoskeletal Pain
The body is constantly sending signals about its health. One of the most easily recognized signals is pain. Musculoskeletal conditions comprise one of the leading causes of severe long-term pain in patients. The musculoskeletal system is an elaborate system of interconnected levers that provides the body with support and mobility. Because of the interconnectedness of the musculoskeletal system, identifying the causes of pain can be challenging. Accurately interpreting the cause of musculoskeletal pain requires an assessment process informed by patient history and physical exams.
In this Discussion, you will consider case studies that describe abnormal findings in patients seen in a clinical setting.
To prepare:
· Your Discussion post should be in the Episodic/Focused SOAP Note format rather than the traditional narrative style Discussion posting format. Refer to Chapter 2 of the Sullivan text and the Episodic/Focused SOAP Template in the Week 5 Learning Resources for guidance. Remember that all Episodic/Focused SOAP notes have specific data included in every patient case.
· Review the following case studies:
With regard to the case study, you were assigned:
· Review this week's Learning Resources and consider the insights they provide about the case study.
· Consider what history would be necessary to collect from the patient in the case study you were assigned.
· Consider what physical exams and diagnostic tests would be appropriate to gather more information about the patient's condition. How would the results be used to make a diagnosis?
· Identify at least five possible conditions that may be considered in a differential diagnosis for the patient.
Note: Before you submit your initial post, replace the subject line ("Discussion - Week 8") with "Review of Case Study ___." Fill in the blank with the number of the case study you were assigned.
By Day 3 of Week 8
Post an episodic/focused note about the patient in the case study to which you were assigned using the episodic/focused note template provided in the Week 5 resources. Provide evidence from the literature to support diagnostic tests that would be appropriate for each case. List five different possible conditions for the patient's differential diagnosis, and justify why you selected each.
Note: For this Discussion, you are required to complete your initial post before you will be able to view and respond to your colleagues’ postings. Begin by clicking on the "Post to Discussion Question" link, and then select "Create Thread" to complete your initial post. Remember, once you click on Submit, you cannot delete or edit your own posts, and you cannot post anonymously. Please check your post carefully before clicking on Submit!
The Case study: Knee Pain
Photo Credit: University of Virginia. (n.d.). N ...
Journal editors evaluate all manuscripts that are submitted to their journal, select those which they consider to be suitable for the journal to send for peer review, and consider peer reviewers’ advice to make a final decision about what gets published. Therefore, it is important to know how they make their decisions.
This presentation gives an insight of what is a review paper,
Purpose & Objective, functions of Review Articles, Difference between Research and Review Articles, Types of Review Articles
Key points to write Review Article, Writing a Scientific Literature Review, Parts of review paper, elements of a review paper, how to write different sections of a review article
Chapter 1 IntroductionBackgroundIntroductionIn this section, .docxketurahhazelhurst
Chapter 1 Introduction
Background/Introduction
In this section, present enough information about the proposed work such that the reader understands the general context or setting. It is also helpful to include a summary of how the rest of this document is organized.
Problem Statement
In this section, present a concise statement of a research-worthy problem addressed (i.e., why the work should be undertaken – don’t say required for the class). Follow the statement of the problem with a well-supported discussion of its scope and nature. The discussion of the problem should include: what the problem is, why it is a problem, how the problem evolved or developed, and the issues and events leading to the problem.
Goal
Next, include a concise definition of the goal of the work (i.e., what the work will accomplish). Aim to define a goal that is measurable.
Research Questions
Research questions are developed to help guide the authors through the literature for a given problem area. What were the open-ended questions asked and why did the student find (or not find) them adequate.
Relevance and Significance
The student should consider the following questions as they read through an article stating how the author(s) supported, or left unsupported the evidence, relevance, and significance of their research literature:
Why is there a problem? What groups or individuals are affected?
How far-ranging is the problem and how great is its impact? What’s the benefit of solving the problem?
What has been tried without success to correct the situation? Why weren’t those attempts successful? What are the consequences of not solving the problem?
How does the goal of the study address the research problem and how will the proposed study offer promise as a resolution to the problem?
How will the research add to the knowledge base?
What is the potential for generalization of the results?
What is the potential for original work?
Barriers and Issues
In these paragraphs, identify how the problem is inherently difficult to solve. How did the solution the author(s) propose address the difficulties?
Chapter 2 Literature Review
In this section, it is important to clearly identify the major areas on which the student will need to focus the student research in order to build a solid foundation for the study in the existing body of knowledge. The literature review is the presentation of quality literature in a particular field that serves as the foundation and justification for the research problem, research questions or hypothesis, and methodology. The student will develop a more comprehensive review of the literature as part of the research.
Chapter 3 Approach/Methodology
This chapter includes a summary of how the student are going to proceed with the evaluation of the problem statement and associated research question(s). Given the short time of this course, a compare / contrast or advantage / disadvantage analysis is recommended that
Chapter 4: Findings, ...
Compare and Contrast Essay AssignmentA Compare and Contrast essaLynellBull52
Compare and Contrast Essay Assignment
A Compare and Contrast essay explores the similarities and differences between two or more items, ideas, topics, trends, works, etc. When we compare, we point out the similarities between two items, and when we contrast, we show their differences. In fact, Compare and Contrast is one of the main rhetorical strategies that writers use to develop ideas and support their arguments.
For this assignment, you will write a Compare and Contrast essay in which you will
explore the similarities and differences between two of the following topics:
1. Generation Z vs. Millennials
2. Generation Z vs. Generation X
3. How Generation Z is perceived vs How Generation Z really is according to you
The American Family Then and Now
1. Compare and Contrast the way and times in which you were raised to that of children today. Do you think that your parent’s were more strict or concerned than today’s parents? Do you think that kids today expect too much? Provide specific examples as to how kids today are being raised similarly and differently than you.
2. How has the role of the woman changed in the family? Is this change good for families? How has the emergence of women in the workforce and their becoming "bread winners" affected the family?
3. As I look at the male figures in my own family, I can see that the role of the male has changed in two generations. My grandfather, for example, has never changed a diaper or cooked a meal in fifty-two years of marriage, yet I do these two things often. In your own family, how has the role of your gender changed in the last two generations. While the changes are probably many, there are still some things that have probably stayed the same. In considering this topic, be sure to include some of the things that are similar regarding the role of your gender in your family structure.
4. More and more couples are choosing not to be married. How and why is this different from the past? Why are people not getting married? How does the affect the family?
5. Non-traditional families are becoming the new normal in the United States. Discuss and compare the traditional family and non-traditional families.
Your purpose for this essay will be to simply inform your audience on their similarities and differences, on the relative merits of the items discussed, and establish the significance of this comparison and contrast. This last point will be expressed in the thesis of your essay which will also strengthen your essay and clarify its purpose.
Process
The first thing you want to do is brainstorm everything you know about each topic, research both of them, go back and look for connections that show similarities and differences, and then develop your thesis. Remember to select only those aspects that are explicitly comparable or contrastable. After you have formulated your thesis statement, established your basis of comparison, and selected your points for discussion, you are ready to organ ...
HLTH 630Article Review 3During this moduleweek you will fin.docxpooleavelina
HLTH 630
Article Review 3
During this module/week you will find an article dealing with the development and/or performance of a needs assessment. This may be an article about a community health assessment or about assessing the special needs of a population afflicted with a particular ailment. The article must come from the American Journal of Preventive Medicine, American Journal of Public Health, or another peer-reviewed source. You will read the article, evaluate needs assessment methodologies, briefly summarize its contents, and compare the findings to the assigned readings from Healthy People 2020 and other literature dealing with needs assessment methodologies.
Title Page
The primary goal of a title should be to summarize the main idea(s) of your paper simply and clearly. The title should be positioned in the upper half of the page and centered. Do not bold, underline, or italicize the title.
Article Abstract
You will write your own abstract for the article, using the author's abstract as a model. You are to write an original abstract (short article summary) of no more than 100 words. Include your first name, middle initial(s), and last name. Omit all titles (e.g. Dr., Professor) and degrees (e.g. MA, PhD, EdD), if applicable. Center and double space your name and the name of your college, university, or institution. The abstract should be on the title page.
Article Review
Include the following headings in your article review section:
Introduction
In this section, discuss the following: Statement of the Problem; Purpose of the Study; Research Questions or Hypotheses and Limitations (if any).
Methodolgy
This section will include Methods; Participants; Data Collection Procedures and Data Analysis Procedures. You will also discuss the quality of the 'literature review' your author completed before they wrote the article. In some cases, you will find a section that discusses the literature reviewed for the study. In other articles, the information is dispersed throughout the article. Discuss the following in this section: Was literature review present? Was it useful? Rigorous? What did you learn? Were there surprises?
Results
Report the results of the study as clearly as possible. If your article reported a quantitative study, report the results in numbers with a good level of detail. If this was a qualitative study, also report the outcomes of the study, being specific about emerging themes, topics, etc.
Conclusions and Recommendations
Did the researcher come to any conclusions or make recommendations? Sometimes this section is given a heading that says: Implications. This is the “so what” section of the research study. What does it mean to practice? Expected length of article review: 2-3 pages. In your review, describe how your selected article addresses each of the sections listed above.
Discussion
Begin this section on a new page, following your conlcusions/recommendations. Expected length of your di ...
In this SLP, you will prepare a presentation in which you provide .docxbradburgess22840
In this SLP, you will prepare a presentation in which you provide your audience with information on the following areas:
1. Introduce the immune system.
2. Explain the differences between antibody mediated immunity and cell mediated immunity.
3. List one example of antibody mediated and cell mediated immunity.
ASSIGNMENT EXPECTATIONS
Number of Slides: 15
Content of Slides: Your presentation should address each of the areas listed above.
Speaker Notes
Each slide will contain speaker notes sufficient to tell me what the purpose of each slide is—specifically, what you would say to a live audience that you were presenting your program to.
SLP: Second Part
Many healthcare programs have modified their operational design and culture to one of being patient-centered while being fiscally viable. As part of your interview of a healthcare manager or executive selected for module 1 discuss how the program was or will be transformed to be patient-centered. In your discussion please address the following questions.
1. How was the program restructured or reengineered to adapt to internal and external factors impacting it?
2. What internal and external factors were considered in the transformation?
3. What were the barriers or obstacles were encountered (e.g. internal politics, economics, resource limitations, time constraints, etc.).
4. What is the potential impact on the program of technology, legislation, etc on the services provided on the program.
*Please note that you may add any additional questions that tie into the themes presented in module 2.
Expectations
Assignment Expectations:
1. Be sure to conduct additional research to gather sufficient information to familiarize yourself with the facts of the case and be sure to justify/support your position.
2. Limit your responses to a maximum of three pages.
3. Please support your discussions with scholarly support (3-5 references). Be sure to properly cite all references.
4. Apply critical thinking skills the the assignment component.
5. I will grade your paper on your ability to address the assignment criteria listed above with depth and breadth of discussion. I will also critique your writing format (i.e. proper reference citations, spacing, etc.).
Your case paper will be further evaluated based on the following criteria:
Precision (Excellent; Good; Average; Poor)
· Each question and or assignment requirement is addressed in the paper
· Accuracy of your answers, key points and supporting discussion
Clarity (Excellent; Good; Average; Poor)
· The paper is well organized, concise, reads clearly, and it is not confusing.
Breadth (Excellent; Good; Average; Poor)
· The paper presents appropriate breadth covering the assignment questions/requirements
Depth (Excellent; Good; Average; Poor)
· Presents key points that lead to deeper matters and issues
· Integrates several points into coherent conclusions
Critical Thinking (Excellent; Good; Average; Poor)
· The paper demonstrates good under.
1. Article Classification (this value is used for classificationAbbyWhyte974
1. Article Classification
(this value is used for classification rather than scoring purpose)
No
Somewhat
Yes
Yes, it is the most important contribution
Does the manuscript identify a research question or set of theories of potential interest to managers?
Does the manuscript specify the academic perspective on the question or theories?
Does the manuscript present one or more descriptions of real-world situations related to the questions/theories employing a narrative format?
Does the manuscript analyze how the situations conform or fail to conform to the existing perspectives?
Does this manuscript identify the management implications of the situation or situations described and their relationship to theory?
2. Manuscript Ratings
This section contains key review questions presented as a scale
Sufficiency of tag line ( is the tagline engaging and descriptive)
Needs substantial rewriting Needs tuning Satisfactory as is Will sell the article! Not Provided
Adequacy of keywords ( Will the keywords adequately aid in search )
Too few or too general Additional keywords would be useful Satisfactory as is Will make the article easy to find! Not Provided
Quality of executive summary Does the executive summary capture the essence of the article and make the potential reader want to dig deeper
Does not convey the sense of the article, or is too difficult to understand Should be tuned further to convey the message and engage the potential reader Satisfactory as is Will ensure that the potential reader becomes an actual reader! Not Provided
Missing
Weak
Adequate but could be improved
Solid
Outstanding
The significance of the issues being considered
( Dose the manuscript present a story that deals with an important issue )
The degree to which the outcomes of the story are likely to be either novel or confusing to practicing managers
(dose the manuscript present a story that readers are unlikely to be familiar with
The degree to which the author’s analysis or theory being presented offers a convincing explanation for the findings
Does the case provide a strong and logical explanation for what is observed in the case?
Sufficiency of the reviewer appendix does the manuscripts reviewer appendix present enough information on the background of the research the conduct of the literature review and the methodology to administrate the rigor behind the research
Summary: Is the writing itself of sufficient quality for publication? Pacifically we are interested in grammar spelling and follow
No Barely With some improvement Yes
No
Barely
With some improvement
Yes
Summary: Is the manuscript sufficiently well organized to warrant publication? (are you able to follow the sequence of the ideas presented?)
Summary: Are the ideas being conveyed by the manuscript interesting? (were you ...
1. Article Classification (this value is used for classificationMartineMccracken314
1. Article Classification
(this value is used for classification rather than scoring purpose)
No
Somewhat
Yes
Yes, it is the most important contribution
Does the manuscript identify a research question or set of theories of potential interest to managers?
Does the manuscript specify the academic perspective on the question or theories?
Does the manuscript present one or more descriptions of real-world situations related to the questions/theories employing a narrative format?
Does the manuscript analyze how the situations conform or fail to conform to the existing perspectives?
Does this manuscript identify the management implications of the situation or situations described and their relationship to theory?
2. Manuscript Ratings
This section contains key review questions presented as a scale
Sufficiency of tag line ( is the tagline engaging and descriptive)
Needs substantial rewriting Needs tuning Satisfactory as is Will sell the article! Not Provided
Adequacy of keywords ( Will the keywords adequately aid in search )
Too few or too general Additional keywords would be useful Satisfactory as is Will make the article easy to find! Not Provided
Quality of executive summary Does the executive summary capture the essence of the article and make the potential reader want to dig deeper
Does not convey the sense of the article, or is too difficult to understand Should be tuned further to convey the message and engage the potential reader Satisfactory as is Will ensure that the potential reader becomes an actual reader! Not Provided
Missing
Weak
Adequate but could be improved
Solid
Outstanding
The significance of the issues being considered
( Dose the manuscript present a story that deals with an important issue )
The degree to which the outcomes of the story are likely to be either novel or confusing to practicing managers
(dose the manuscript present a story that readers are unlikely to be familiar with
The degree to which the author’s analysis or theory being presented offers a convincing explanation for the findings
Does the case provide a strong and logical explanation for what is observed in the case?
Sufficiency of the reviewer appendix does the manuscripts reviewer appendix present enough information on the background of the research the conduct of the literature review and the methodology to administrate the rigor behind the research
Summary: Is the writing itself of sufficient quality for publication? Pacifically we are interested in grammar spelling and follow
No Barely With some improvement Yes
No
Barely
With some improvement
Yes
Summary: Is the manuscript sufficiently well organized to warrant publication? (are you able to follow the sequence of the ideas presented?)
Summary: Are the ideas being conveyed by the manuscript interesting? (were you ...
This session offers insights into the reviewing process associated with academic journals, which will help you in the roles of both reviewer and author. It will offer advice and reflection on when to accept an invitation to review, and on the benefits and practicalities of the process.
EBP Journal ArticleLeadership Team What kind oEvonCanales257
EBP Journal Article
Leadership Team
What kind of Article do I pick?
First review the group project problem and title.
Use key words from your problem for your lookup inquiry. i.e. infection control, nurse, retention, etc.
Use EBP articles no more than 5 years old.
Make sure you put your name at the top and include your article citation in 7th ed. APA format of your assignment - see exemplar.
EBP Journal Article in APA Format
Example:
Student Name: XXXXXXXX
EPB Journal Article in APA format:
Sánchez, M., Suárez, M., Asenjo, M., & Bragulat, E. (2018). Improvement of emergency department patient flow using lean thinking. International Journal For Quality In Health Care: Journal Of The International Society For Quality In Health Care, 30(4), 250–256. https://doi.org/10.1093/intqhc/mzy017
(- Authors – Last name, first initial. Followed by Date in ( parenthesis); Followed by a period. Journal article starts with a capital letter and all other words are lowercase except for words following a colon, semicolon or period within the title. Journal title each letter at the beginning od the word is a capital. This is followed by the volume, chapter and pages. https://www.scribbr.com/apa-style/in-text-citation/ )
2 points - EBP
thE Problem and The Goal
SWOT – “S” is for strengths
Strength - Example: Did they have support of the peers, manager, CEO. Did this project improve patient satisfaction, quality, efficiency? Usually found in the literature review and results
7
SWOT – “W” is for Weakness
Weakness - Example: This is the opposite. Why was this weak? Not enough education, no support, not enough money because of the expense? Usually found in the problem of the literature review, results and limitations.
8
Swot – “O” is for Opportunity
Opportunity - Example: If there wasn't enough education, is this an opportunity? A better survey or tool? Usually found in results or limitation and future implications.
9
Swot – “t” is for Threats
Threat - Example: Increased infection, possible death, etc. Usually found in the literature review.
10
Title:
Documentation of problem based assessment of the gastrointestinal system.
Purpose of Assignment:
Learning the required components of documenting a problem based subjective and objective assessment of gastrointestinal system. Identify abnormal findings.
Course Competency:
Prioritize appropriate assessment techniques for the gastrointestinal, breasts, and genitourinary systems.
Instructions:
Content: Use of three sections:
· Subjective
· Objective
· Actual or potential risk factors for the client based on the assessment findings with description or reason for selection of them.
Format:
· Standard American English (correct grammar, punctuation, etc.)
Documentation Grading Rubric- 10 possible points
Levels of Achievement
Criteria
Emerging
Competence
Proficiency
Mastery
Subjective
(4 Pts)
Missing components such as biographic data, medications, or aller ...
Aaa assessment 2/Assessment description.pdf
Critical Thinking and Managerial Decision Making
Written Assessment- Reflective Essay
Task: The essay must be 2000 words (+/-10%) in length.
Cover page: The essay must include a cover page that contains your name, student number,
resident campus, assessment title, and lecturer.
Format: Text should be word-processed, with appropriate layout and use of headings/sub
headings. Times New Roman, 12 size font and line spacing (1.5).
Referencing: A minimum of 10 academic references are required. The list of references
should form the last page or two, at the end of the assessment. Referencing should be in a
consistent APA style.
Task Description: The objectives of this reflective essay are for students to (1) summarize,
critically review and reinforce key concepts and theories learned from week 1 to week 9 of this
unit. (2) reflect on these concepts/theories’ potential impact on students’ decision making, and
how these learnings are being applied and could be applied in their current jobs, development
of future career and/or their life generally. (3) reflect on new insights gained about oneself as a
result of learning the unit and propose a plan for self-development.
Minimum number of concepts/theories/themes required to pass: 6
Students ought to start the reflective essay in week 4 after the submission of their first
assignment. From week 4 to week 9 (a total of 6 weeks), students are expected to reflect on
the topics (week 1-week 9) this unit has covered and evaluate which concepts or theories
have inspired their thinking or influenced their decision making. This assignment still
requires an essay format with introduction section outlining the scope, purpose and
structure information. The body paragraphs need to contain at least six themes over the
course of six weeks, and each theme needs to contain a CONVINCING and PERSONAL
justification of why these concepts/theories are chosen and how the mentioned
concepts/theories have changed/might change the students’ thinking or the students’
decisions. For example, students could identify the concepts of “automatic versus critical
thinking” as a theme for one week’s reflection, and illustrate with personal examples how
having learned about the differences has enabled him/her to be more effective in making a
certain decision at work. Close to the end, the essay needs to reflect on self-knowledge and
highlight implications for future self-development.
To successfully complete this assessment task, students should answer the following reflective essay
questions:
Which concepts/theories from which week’s learning activities are significant or important to
you?
Why are these concepts/theories you have identified important or significant to you?
(Theoretical review)
How are the concepts/theories you have learned influencing/impacting your deci.
Journal editors evaluate all manuscripts that are submitted to their journal, select those which they consider to be suitable for the journal to send for peer review, and consider peer reviewers’ advice to make a final decision about what gets published. Therefore, it is important to know how they make their decisions.
This presentation gives an insight of what is a review paper,
Purpose & Objective, functions of Review Articles, Difference between Research and Review Articles, Types of Review Articles
Key points to write Review Article, Writing a Scientific Literature Review, Parts of review paper, elements of a review paper, how to write different sections of a review article
Chapter 1 IntroductionBackgroundIntroductionIn this section, .docxketurahhazelhurst
Chapter 1 Introduction
Background/Introduction
In this section, present enough information about the proposed work such that the reader understands the general context or setting. It is also helpful to include a summary of how the rest of this document is organized.
Problem Statement
In this section, present a concise statement of a research-worthy problem addressed (i.e., why the work should be undertaken – don’t say required for the class). Follow the statement of the problem with a well-supported discussion of its scope and nature. The discussion of the problem should include: what the problem is, why it is a problem, how the problem evolved or developed, and the issues and events leading to the problem.
Goal
Next, include a concise definition of the goal of the work (i.e., what the work will accomplish). Aim to define a goal that is measurable.
Research Questions
Research questions are developed to help guide the authors through the literature for a given problem area. What were the open-ended questions asked and why did the student find (or not find) them adequate.
Relevance and Significance
The student should consider the following questions as they read through an article stating how the author(s) supported, or left unsupported the evidence, relevance, and significance of their research literature:
Why is there a problem? What groups or individuals are affected?
How far-ranging is the problem and how great is its impact? What’s the benefit of solving the problem?
What has been tried without success to correct the situation? Why weren’t those attempts successful? What are the consequences of not solving the problem?
How does the goal of the study address the research problem and how will the proposed study offer promise as a resolution to the problem?
How will the research add to the knowledge base?
What is the potential for generalization of the results?
What is the potential for original work?
Barriers and Issues
In these paragraphs, identify how the problem is inherently difficult to solve. How did the solution the author(s) propose address the difficulties?
Chapter 2 Literature Review
In this section, it is important to clearly identify the major areas on which the student will need to focus the student research in order to build a solid foundation for the study in the existing body of knowledge. The literature review is the presentation of quality literature in a particular field that serves as the foundation and justification for the research problem, research questions or hypothesis, and methodology. The student will develop a more comprehensive review of the literature as part of the research.
Chapter 3 Approach/Methodology
This chapter includes a summary of how the student are going to proceed with the evaluation of the problem statement and associated research question(s). Given the short time of this course, a compare / contrast or advantage / disadvantage analysis is recommended that
Chapter 4: Findings, ...
Compare and Contrast Essay AssignmentA Compare and Contrast essaLynellBull52
Compare and Contrast Essay Assignment
A Compare and Contrast essay explores the similarities and differences between two or more items, ideas, topics, trends, works, etc. When we compare, we point out the similarities between two items, and when we contrast, we show their differences. In fact, Compare and Contrast is one of the main rhetorical strategies that writers use to develop ideas and support their arguments.
For this assignment, you will write a Compare and Contrast essay in which you will
explore the similarities and differences between two of the following topics:
1. Generation Z vs. Millennials
2. Generation Z vs. Generation X
3. How Generation Z is perceived vs How Generation Z really is according to you
The American Family Then and Now
1. Compare and Contrast the way and times in which you were raised to that of children today. Do you think that your parent’s were more strict or concerned than today’s parents? Do you think that kids today expect too much? Provide specific examples as to how kids today are being raised similarly and differently than you.
2. How has the role of the woman changed in the family? Is this change good for families? How has the emergence of women in the workforce and their becoming "bread winners" affected the family?
3. As I look at the male figures in my own family, I can see that the role of the male has changed in two generations. My grandfather, for example, has never changed a diaper or cooked a meal in fifty-two years of marriage, yet I do these two things often. In your own family, how has the role of your gender changed in the last two generations. While the changes are probably many, there are still some things that have probably stayed the same. In considering this topic, be sure to include some of the things that are similar regarding the role of your gender in your family structure.
4. More and more couples are choosing not to be married. How and why is this different from the past? Why are people not getting married? How does the affect the family?
5. Non-traditional families are becoming the new normal in the United States. Discuss and compare the traditional family and non-traditional families.
Your purpose for this essay will be to simply inform your audience on their similarities and differences, on the relative merits of the items discussed, and establish the significance of this comparison and contrast. This last point will be expressed in the thesis of your essay which will also strengthen your essay and clarify its purpose.
Process
The first thing you want to do is brainstorm everything you know about each topic, research both of them, go back and look for connections that show similarities and differences, and then develop your thesis. Remember to select only those aspects that are explicitly comparable or contrastable. After you have formulated your thesis statement, established your basis of comparison, and selected your points for discussion, you are ready to organ ...
HLTH 630Article Review 3During this moduleweek you will fin.docxpooleavelina
HLTH 630
Article Review 3
During this module/week you will find an article dealing with the development and/or performance of a needs assessment. This may be an article about a community health assessment or about assessing the special needs of a population afflicted with a particular ailment. The article must come from the American Journal of Preventive Medicine, American Journal of Public Health, or another peer-reviewed source. You will read the article, evaluate needs assessment methodologies, briefly summarize its contents, and compare the findings to the assigned readings from Healthy People 2020 and other literature dealing with needs assessment methodologies.
Title Page
The primary goal of a title should be to summarize the main idea(s) of your paper simply and clearly. The title should be positioned in the upper half of the page and centered. Do not bold, underline, or italicize the title.
Article Abstract
You will write your own abstract for the article, using the author's abstract as a model. You are to write an original abstract (short article summary) of no more than 100 words. Include your first name, middle initial(s), and last name. Omit all titles (e.g. Dr., Professor) and degrees (e.g. MA, PhD, EdD), if applicable. Center and double space your name and the name of your college, university, or institution. The abstract should be on the title page.
Article Review
Include the following headings in your article review section:
Introduction
In this section, discuss the following: Statement of the Problem; Purpose of the Study; Research Questions or Hypotheses and Limitations (if any).
Methodolgy
This section will include Methods; Participants; Data Collection Procedures and Data Analysis Procedures. You will also discuss the quality of the 'literature review' your author completed before they wrote the article. In some cases, you will find a section that discusses the literature reviewed for the study. In other articles, the information is dispersed throughout the article. Discuss the following in this section: Was literature review present? Was it useful? Rigorous? What did you learn? Were there surprises?
Results
Report the results of the study as clearly as possible. If your article reported a quantitative study, report the results in numbers with a good level of detail. If this was a qualitative study, also report the outcomes of the study, being specific about emerging themes, topics, etc.
Conclusions and Recommendations
Did the researcher come to any conclusions or make recommendations? Sometimes this section is given a heading that says: Implications. This is the “so what” section of the research study. What does it mean to practice? Expected length of article review: 2-3 pages. In your review, describe how your selected article addresses each of the sections listed above.
Discussion
Begin this section on a new page, following your conlcusions/recommendations. Expected length of your di ...
In this SLP, you will prepare a presentation in which you provide .docxbradburgess22840
In this SLP, you will prepare a presentation in which you provide your audience with information on the following areas:
1. Introduce the immune system.
2. Explain the differences between antibody mediated immunity and cell mediated immunity.
3. List one example of antibody mediated and cell mediated immunity.
ASSIGNMENT EXPECTATIONS
Number of Slides: 15
Content of Slides: Your presentation should address each of the areas listed above.
Speaker Notes
Each slide will contain speaker notes sufficient to tell me what the purpose of each slide is—specifically, what you would say to a live audience that you were presenting your program to.
SLP: Second Part
Many healthcare programs have modified their operational design and culture to one of being patient-centered while being fiscally viable. As part of your interview of a healthcare manager or executive selected for module 1 discuss how the program was or will be transformed to be patient-centered. In your discussion please address the following questions.
1. How was the program restructured or reengineered to adapt to internal and external factors impacting it?
2. What internal and external factors were considered in the transformation?
3. What were the barriers or obstacles were encountered (e.g. internal politics, economics, resource limitations, time constraints, etc.).
4. What is the potential impact on the program of technology, legislation, etc on the services provided on the program.
*Please note that you may add any additional questions that tie into the themes presented in module 2.
Expectations
Assignment Expectations:
1. Be sure to conduct additional research to gather sufficient information to familiarize yourself with the facts of the case and be sure to justify/support your position.
2. Limit your responses to a maximum of three pages.
3. Please support your discussions with scholarly support (3-5 references). Be sure to properly cite all references.
4. Apply critical thinking skills the the assignment component.
5. I will grade your paper on your ability to address the assignment criteria listed above with depth and breadth of discussion. I will also critique your writing format (i.e. proper reference citations, spacing, etc.).
Your case paper will be further evaluated based on the following criteria:
Precision (Excellent; Good; Average; Poor)
· Each question and or assignment requirement is addressed in the paper
· Accuracy of your answers, key points and supporting discussion
Clarity (Excellent; Good; Average; Poor)
· The paper is well organized, concise, reads clearly, and it is not confusing.
Breadth (Excellent; Good; Average; Poor)
· The paper presents appropriate breadth covering the assignment questions/requirements
Depth (Excellent; Good; Average; Poor)
· Presents key points that lead to deeper matters and issues
· Integrates several points into coherent conclusions
Critical Thinking (Excellent; Good; Average; Poor)
· The paper demonstrates good under.
1. Article Classification (this value is used for classificationAbbyWhyte974
1. Article Classification
(this value is used for classification rather than scoring purpose)
No
Somewhat
Yes
Yes, it is the most important contribution
Does the manuscript identify a research question or set of theories of potential interest to managers?
Does the manuscript specify the academic perspective on the question or theories?
Does the manuscript present one or more descriptions of real-world situations related to the questions/theories employing a narrative format?
Does the manuscript analyze how the situations conform or fail to conform to the existing perspectives?
Does this manuscript identify the management implications of the situation or situations described and their relationship to theory?
2. Manuscript Ratings
This section contains key review questions presented as a scale
Sufficiency of tag line ( is the tagline engaging and descriptive)
Needs substantial rewriting Needs tuning Satisfactory as is Will sell the article! Not Provided
Adequacy of keywords ( Will the keywords adequately aid in search )
Too few or too general Additional keywords would be useful Satisfactory as is Will make the article easy to find! Not Provided
Quality of executive summary Does the executive summary capture the essence of the article and make the potential reader want to dig deeper
Does not convey the sense of the article, or is too difficult to understand Should be tuned further to convey the message and engage the potential reader Satisfactory as is Will ensure that the potential reader becomes an actual reader! Not Provided
Missing
Weak
Adequate but could be improved
Solid
Outstanding
The significance of the issues being considered
( Dose the manuscript present a story that deals with an important issue )
The degree to which the outcomes of the story are likely to be either novel or confusing to practicing managers
(dose the manuscript present a story that readers are unlikely to be familiar with
The degree to which the author’s analysis or theory being presented offers a convincing explanation for the findings
Does the case provide a strong and logical explanation for what is observed in the case?
Sufficiency of the reviewer appendix does the manuscripts reviewer appendix present enough information on the background of the research the conduct of the literature review and the methodology to administrate the rigor behind the research
Summary: Is the writing itself of sufficient quality for publication? Pacifically we are interested in grammar spelling and follow
No Barely With some improvement Yes
No
Barely
With some improvement
Yes
Summary: Is the manuscript sufficiently well organized to warrant publication? (are you able to follow the sequence of the ideas presented?)
Summary: Are the ideas being conveyed by the manuscript interesting? (were you ...
1. Article Classification (this value is used for classificationMartineMccracken314
1. Article Classification
(this value is used for classification rather than scoring purpose)
No
Somewhat
Yes
Yes, it is the most important contribution
Does the manuscript identify a research question or set of theories of potential interest to managers?
Does the manuscript specify the academic perspective on the question or theories?
Does the manuscript present one or more descriptions of real-world situations related to the questions/theories employing a narrative format?
Does the manuscript analyze how the situations conform or fail to conform to the existing perspectives?
Does this manuscript identify the management implications of the situation or situations described and their relationship to theory?
2. Manuscript Ratings
This section contains key review questions presented as a scale
Sufficiency of tag line ( is the tagline engaging and descriptive)
Needs substantial rewriting Needs tuning Satisfactory as is Will sell the article! Not Provided
Adequacy of keywords ( Will the keywords adequately aid in search )
Too few or too general Additional keywords would be useful Satisfactory as is Will make the article easy to find! Not Provided
Quality of executive summary Does the executive summary capture the essence of the article and make the potential reader want to dig deeper
Does not convey the sense of the article, or is too difficult to understand Should be tuned further to convey the message and engage the potential reader Satisfactory as is Will ensure that the potential reader becomes an actual reader! Not Provided
Missing
Weak
Adequate but could be improved
Solid
Outstanding
The significance of the issues being considered
( Dose the manuscript present a story that deals with an important issue )
The degree to which the outcomes of the story are likely to be either novel or confusing to practicing managers
(dose the manuscript present a story that readers are unlikely to be familiar with
The degree to which the author’s analysis or theory being presented offers a convincing explanation for the findings
Does the case provide a strong and logical explanation for what is observed in the case?
Sufficiency of the reviewer appendix does the manuscripts reviewer appendix present enough information on the background of the research the conduct of the literature review and the methodology to administrate the rigor behind the research
Summary: Is the writing itself of sufficient quality for publication? Pacifically we are interested in grammar spelling and follow
No Barely With some improvement Yes
No
Barely
With some improvement
Yes
Summary: Is the manuscript sufficiently well organized to warrant publication? (are you able to follow the sequence of the ideas presented?)
Summary: Are the ideas being conveyed by the manuscript interesting? (were you ...
This session offers insights into the reviewing process associated with academic journals, which will help you in the roles of both reviewer and author. It will offer advice and reflection on when to accept an invitation to review, and on the benefits and practicalities of the process.
EBP Journal ArticleLeadership Team What kind oEvonCanales257
EBP Journal Article
Leadership Team
What kind of Article do I pick?
First review the group project problem and title.
Use key words from your problem for your lookup inquiry. i.e. infection control, nurse, retention, etc.
Use EBP articles no more than 5 years old.
Make sure you put your name at the top and include your article citation in 7th ed. APA format of your assignment - see exemplar.
EBP Journal Article in APA Format
Example:
Student Name: XXXXXXXX
EPB Journal Article in APA format:
Sánchez, M., Suárez, M., Asenjo, M., & Bragulat, E. (2018). Improvement of emergency department patient flow using lean thinking. International Journal For Quality In Health Care: Journal Of The International Society For Quality In Health Care, 30(4), 250–256. https://doi.org/10.1093/intqhc/mzy017
(- Authors – Last name, first initial. Followed by Date in ( parenthesis); Followed by a period. Journal article starts with a capital letter and all other words are lowercase except for words following a colon, semicolon or period within the title. Journal title each letter at the beginning od the word is a capital. This is followed by the volume, chapter and pages. https://www.scribbr.com/apa-style/in-text-citation/ )
2 points - EBP
thE Problem and The Goal
SWOT – “S” is for strengths
Strength - Example: Did they have support of the peers, manager, CEO. Did this project improve patient satisfaction, quality, efficiency? Usually found in the literature review and results
7
SWOT – “W” is for Weakness
Weakness - Example: This is the opposite. Why was this weak? Not enough education, no support, not enough money because of the expense? Usually found in the problem of the literature review, results and limitations.
8
Swot – “O” is for Opportunity
Opportunity - Example: If there wasn't enough education, is this an opportunity? A better survey or tool? Usually found in results or limitation and future implications.
9
Swot – “t” is for Threats
Threat - Example: Increased infection, possible death, etc. Usually found in the literature review.
10
Title:
Documentation of problem based assessment of the gastrointestinal system.
Purpose of Assignment:
Learning the required components of documenting a problem based subjective and objective assessment of gastrointestinal system. Identify abnormal findings.
Course Competency:
Prioritize appropriate assessment techniques for the gastrointestinal, breasts, and genitourinary systems.
Instructions:
Content: Use of three sections:
· Subjective
· Objective
· Actual or potential risk factors for the client based on the assessment findings with description or reason for selection of them.
Format:
· Standard American English (correct grammar, punctuation, etc.)
Documentation Grading Rubric- 10 possible points
Levels of Achievement
Criteria
Emerging
Competence
Proficiency
Mastery
Subjective
(4 Pts)
Missing components such as biographic data, medications, or aller ...
Aaa assessment 2/Assessment description.pdf
Critical Thinking and Managerial Decision Making
Written Assessment- Reflective Essay
Task: The essay must be 2000 words (+/-10%) in length.
Cover page: The essay must include a cover page that contains your name, student number,
resident campus, assessment title, and lecturer.
Format: Text should be word-processed, with appropriate layout and use of headings/sub
headings. Times New Roman, 12 size font and line spacing (1.5).
Referencing: A minimum of 10 academic references are required. The list of references
should form the last page or two, at the end of the assessment. Referencing should be in a
consistent APA style.
Task Description: The objectives of this reflective essay are for students to (1) summarize,
critically review and reinforce key concepts and theories learned from week 1 to week 9 of this
unit. (2) reflect on these concepts/theories’ potential impact on students’ decision making, and
how these learnings are being applied and could be applied in their current jobs, development
of future career and/or their life generally. (3) reflect on new insights gained about oneself as a
result of learning the unit and propose a plan for self-development.
Minimum number of concepts/theories/themes required to pass: 6
Students ought to start the reflective essay in week 4 after the submission of their first
assignment. From week 4 to week 9 (a total of 6 weeks), students are expected to reflect on
the topics (week 1-week 9) this unit has covered and evaluate which concepts or theories
have inspired their thinking or influenced their decision making. This assignment still
requires an essay format with introduction section outlining the scope, purpose and
structure information. The body paragraphs need to contain at least six themes over the
course of six weeks, and each theme needs to contain a CONVINCING and PERSONAL
justification of why these concepts/theories are chosen and how the mentioned
concepts/theories have changed/might change the students’ thinking or the students’
decisions. For example, students could identify the concepts of “automatic versus critical
thinking” as a theme for one week’s reflection, and illustrate with personal examples how
having learned about the differences has enabled him/her to be more effective in making a
certain decision at work. Close to the end, the essay needs to reflect on self-knowledge and
highlight implications for future self-development.
To successfully complete this assessment task, students should answer the following reflective essay
questions:
Which concepts/theories from which week’s learning activities are significant or important to
you?
Why are these concepts/theories you have identified important or significant to you?
(Theoretical review)
How are the concepts/theories you have learned influencing/impacting your deci.
Similar to Review form cons rw-17-0047 by reviewer scientria ricerca (20)
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Review form cons rw-17-0047 by reviewer scientria ricerca
1. Article Review Form
Dear Reviewer,
Hope your valuable review helps to propel the quality of the Manuscript.
Manuscript Title: Immune shock - chronologic event in some brain pathology
Category : Review Process
Manuscript ID : CONS-RW-17-0047
Please indicate whether the following criteria are met Yes/No
The article title and abstract are appropriate yes
The purpose of the article is stated clearly yes
The significance of the article is explicitly stated. Yes
The study methods are sound and appropriate Yes
The writing is clear and concise Yes
The conclusions or summary are accurate and supported by the content No
The article is of interest to our target. Yes
Dear Reviewer,
Thank you very much for reviewing this paper. Please indicate your final decision concerning
this paper by choosing any one of the following actions you recommend
Please indicate any one of the following actions you recommend Yes/No
Publish, no revision needed
Publish, but needs minor revision Yes
Publish, but needs major revision
Reject, but encourage author to try a major revision
Reject, ethical issues
Review Comments & Suggestions
Ithas beenexplainedandcenteredon.The topic of thisarticle is very significant
the neurobiological foundationsof the mostcritical pervasivedevelopmental
disorders(Autism).
The researchershouldgoon throughthe followingcomments andmodifications:
1- Keyword shouldbe transferredto be after the "Abstract".
2- The abstract isveryshort.It shouldbe included: The objectives,Method,Most
important findings.
3- The text:shouldbe corrected: statements,paragraphs,
4- Afterpoint,coma: space
5- Titlesandsubtitles:plot.
6- It is veryimportant,scientifically,tomention: pervasive developmental disorder.
7- Results: to be after discussion.
8- Discussion:verypoor explained.
9-References:shouldorganizedaccording toAPA
The resultof evaluation:Acceptedafter minor revision& modifications.