SV 40, introduction of vectors, advantages of sv 40, future prospectus, structure of sv 40 vector

1. GENERAL INFORMATION ONGENERAL INFORMATION ON
SV-40SV-40
MANSI R. MORE
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2. CONTENTS
• Introduction
• SV-40 vector
• Structure
• Advantages
• Future prospects
• References
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3. INTRODUCTION
• Vectors are molecular vehicles which have a direction of transfer
and are used by researchers for the transfer of foreign molecule
into another host cell.
• A cloning vector is a small piece of DNA into which a foreign DNA• A cloning vector is a small piece of DNA into which a foreign DNA
fragment into the cloning vector is carried out by treating the
vehicle and the foreign DNA with the same restriction enzyme,
then ligating the fragments together.
• Genetically engineered plasmids and bacteriophages are perhaps
most commonly used for this purpose.
Vectors are molecular vehicles which have a direction of transfer
the transfer of foreign molecule
A cloning vector is a small piece of DNA into which a foreign DNAA cloning vector is a small piece of DNA into which a foreign DNA
fragment into the cloning vector is carried out by treating the
vehicle and the foreign DNA with the same restriction enzyme,
then ligating the fragments together.
Genetically engineered plasmids and bacteriophages are perhaps
most commonly used for this purpose. 3

4. SV vector
• SV-40 is for Simian vacuolating virus 40 or Simian virus 40, a
found in both monkeys and humans. It was named for the effect it produced
on infected green monkey cells, which developed unusual number of
vacuoles.
• Like other polymaviruses, SV 40 is a DNA virus that has the potential to cause• Like other polymaviruses, SV 40 is a DNA virus that has the potential to cause
tumors.
• SV-40 was first identified by Ben Sweet & Maurice Hilleman 1960 when they
found that between 10-30 % of polio vaccines in USA were contaminated with
SV-40.
• The complete viral genome was sequenced by Walter
40 is for Simian vacuolating virus 40 or Simian virus 40, a polymavirus
found in both monkeys and humans. It was named for the effect it produced
on infected green monkey cells, which developed unusual number of
, SV 40 is a DNA virus that has the potential to cause, SV 40 is a DNA virus that has the potential to cause
40 was first identified by Ben Sweet & Maurice Hilleman 1960 when they
30 % of polio vaccines in USA were contaminated with
The complete viral genome was sequenced by Walter Fiers and his team.
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5. • SV-40 is a spherical virus with a circular, double stranded 5243 bp
chromosomes. Which encodes 5 proteins, viz., small
protein), VP1,VP2 and VP3 (virion protein), has an origin of replication (about
80 bp) and is complexed with histones to form chromatin.
• Large-T is essential for viral replication, while VP1, VP2 and VP3 form the viral
capsid. In laboratory, it is multiplied in cultured kidney cells of African green
monkey; infected cells lyse after 4 days releasingmonkey; infected cells lyse after 4 days releasing
virions/cell.
• SV40 plasmids (vectors) can be packaged only if their DNA is within the range
of 3900 to 5300 bp.
• SV 40 is capable of multiplicity reactivation (MR). MR is the process by which
two, or more, virus genomes containing otherwise lethal damage interact
within an infected cell to form a visible virus genome.
40 is a spherical virus with a circular, double stranded 5243 bp
chromosomes. Which encodes 5 proteins, viz., small-t, large-t (both early
protein), VP1,VP2 and VP3 (virion protein), has an origin of replication (about
80 bp) and is complexed with histones to form chromatin.
T is essential for viral replication, while VP1, VP2 and VP3 form the viral
capsid. In laboratory, it is multiplied in cultured kidney cells of African green
monkey; infected cells lyse after 4 days releasing upto 10 raise to the power 5monkey; infected cells lyse after 4 days releasing upto 10 raise to the power 5
SV40 plasmids (vectors) can be packaged only if their DNA is within the range
SV 40 is capable of multiplicity reactivation (MR). MR is the process by which
two, or more, virus genomes containing otherwise lethal damage interact
within an infected cell to form a visible virus genome.
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6. STRUCTURE
surface envelope protein
transmembrane envelope protein
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membrane
Matrix protein
capsid
RNA genome, bound by nucleocapsid(NC)
Integrase (IN)
Reverse transcriptase (RT)
Protease(PR)

7. Retrovirus Vectors
• Retrovirus have a single-stranded RNA genome. Each virus has 2 copies of
genome, which resemble eukaryotic mRNAs. The viral genome reverse
transcribed by reverse transcriptase into a DNA double strand copy inside the
host cells.
• The following properties of the retroviruses are useful in their use as vectors:
1) A wide host range (Birds, mammals and other animals)
2) Infected cells are not killed and they continue to produce virus particles over
indefinite period.
3) Presence of strong promoters.
4) Regulation of promoter action in case of some viruses.
5) Retroviral vectors are constructed from cloned DNA genomes of retroviruses.
stranded RNA genome. Each virus has 2 copies of
genome, which resemble eukaryotic mRNAs. The viral genome reverse
transcribed by reverse transcriptase into a DNA double strand copy inside the
The following properties of the retroviruses are useful in their use as vectors:
A wide host range (Birds, mammals and other animals)
Infected cells are not killed and they continue to produce virus particles over
Regulation of promoter action in case of some viruses.
Retroviral vectors are constructed from cloned DNA genomes of retroviruses.7

8. CONT…
• These have the following features:
1) The vector has the viral sequences for replication, gene expression and
packaging.
2) DNA inserts may either replace or be located in the non essential coding2) DNA inserts may either replace or be located in the non essential coding
regions of the viral genome.
3) The viral proteins are usually provided by the helper virus or a provirus.
4) DNA copies of the retrovirus genome are used as vectors, generally as shuttle
vectors.
The vector has the viral sequences for replication, gene expression and
DNA inserts may either replace or be located in the non essential codingDNA inserts may either replace or be located in the non essential coding
The viral proteins are usually provided by the helper virus or a provirus.
DNA copies of the retrovirus genome are used as vectors, generally as shuttle
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9. Advantages of using SV 40 as a vector:
• SV40 vectors (SV40) are good candidates for gene transfer, as they display some
unique features. SV 40 is a well –known virus, non
make.
• They also efficiently transduce both, resting and dividing cells, deliver persistent
transgene expression to a wide range of cell types, and are nonimmunogenic.
1. It is easily modified to be non replicative.1. It is easily modified to be non replicative.
2. It can be produced in large quantities.
3. It infects almost every cell type that has been tested, both dividing and quiescent.
4. It is not immunogenic it allows long-term expression of the transgene.
5. Its molecular biology is well studied.
6. The effects in humans of wild-type SV40 have been documented.
Advantages of using SV 40 as a vector:-
SV40 vectors (SV40) are good candidates for gene transfer, as they display some
known virus, non-replicative vectors are easy-to-
They also efficiently transduce both, resting and dividing cells, deliver persistent
transgene expression to a wide range of cell types, and are nonimmunogenic.
It infects almost every cell type that has been tested, both dividing and quiescent.
term expression of the transgene.
type SV40 have been documented.
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10. FUTURE PROSPECTS
• Many ongoing research have been going on to develop
recombinant SV 40 vectors.
• It serves as a potential for new vectors.
• Because of it, retro viruses can be understood in more detail.
• Wide range of selection for gene transfer experiments.
Many ongoing research have been going on to develop
It serves as a potential for new vectors.
Because of it, retro viruses can be understood in more detail.
Wide range of selection for gene transfer experiments.
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11. REFERENCES
• Bibliography
• www.google.com• www.google.com
• www.Wikipedia.org
• http://www.jbc.org
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12. THANK YOUTHANK YOUTHANK YOUTHANK YOU
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