Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

Retroviral Vectors

3,352 views

Published on

retroviral vectors

Published in: Education
  • Be the first to comment

Retroviral Vectors

  1. 1. Retroviral Vectors By: Maryam shakeel
  2. 2. Retrovirus Class of enveloped viruses Single stranded RNA Retroviruses are one of the mainstays of current gene therapy approaches. The recombinant retroviruses such as the Moloney murine leukemia virus have the ability to integrate into the host genome in a stable fashion
  3. 3. Genome of Retrovirus • LTR (long terminal repeat) U5 envpolgag5’ 3’ • PBS (primer binding site) • “Pol” gene • “gag” gene • “env” gene • “Ѱ” packaging signal site • 5’ss & 3’ss site Ѱ 5’ss 3’ss U3 R U5U3 R LTR LTR • PPT (polypurine Test)
  4. 4. Construction of Replication defective Retroviral Vector: • Retroviral vectors are derived from wild type retroviruses (Moloney murine leukaemia provirus) and are engineered to carry a foreign gene of interest into a target cell. • Retroviral vectors are composed of the gene of interest and the cis-acting elements of the viral genome , with the removal of the trans- acting viral genes (gag, pol, and env)
  5. 5. LTR LTRenvpolgag5’ 3’ Ѱ 5’ss 3’ss LTR LTR5’ 3’ Ѱ 5’ss Gene of Interest
  6. 6. Applications in Gene therapy:
  7. 7. Limitations: • Moloney retrovirus involves the requirement for cells to be actively dividing for transduction. • insertional mutagenesis due to integration into the host genome might lead to cancer or leukemia.
  8. 8. Thanks

×