cell culture study , microbiological study of cell , culture media and its types, Basic components of cell culture media , Various types of cell culture media , Types of cell culture

1. C E L L C U LT U R E M E D I A A N D
VA R I O U S T Y P E S O F C E L L
C U LT U R E
Presented by,
Jikhila Machado
2nd sem M.Pharm
Pharmaceutical analysis

2. C U LT U R E M E D I A
o The culture medium is the most important
component of the culture environment
because it provides the necessary nutrients ,
growth factors, and hormones for cell
growth as well as regulating the pH and the
osmotic pressure of the culture.
o TYPES : On the basis of consistency
1. Solid Medium: Contains 1-2% of agar or
other inert solidifying agent that provides a
physical structure to allow the bacteria to
grow in an informative way.
2. Semi Solid Medium: Contains 0.5% or less
agar to facilitate the motility of microbe.
3. Liquid or Broth Medium: Contains no
agar. It is used when a large amount of
microbes is needed to grow.

3. B A S I C C O M P O N E N T S O F
C E L L C U LT U R E M E D I A
 Basal medium
 Serum
 Buffering system.
 Amino acids.
 Vitamins
 Carbohydrates.
 Inorganic salts
 Proteins and peptides
 Fatty acids and lipids
 Trace elements
 Hormones and growth factors
 Antibiotics

4. VA R I O U S T Y P E O F C E L L
C U LT U R E M E D I A
Animal cells can be cultured either using a completely natural medium or an
artificial / synthetic medium along with some natural products

6. C E L L C U LT U R E
o Cell culture refers to the removal of cells.
o The cells may be removed from the tissue directly and
disaggregated by enzymatic or mechanical means before
cultivation.
o They may be derived from a cell line or cell strain that has already
been established

7. T Y P E S O F C E L L
C U LT U R E
o There are four types of animal cell culture.
1. Primary cell culture
2. Secondary cell culture or cell line.
3. Diploid cell strain
4. Continuous cell line

8. P R I M A R Y C E L L
C U LT U R E
o This culture is derived from normal tissue
taken from an animal such as mouse, hamster,
chicken, monkey or human. This tissue is
treated with proteolytic enzymes to give a cell
suspension.
o These cells are then placed along with the
growth medium in a vessel and incubated
o This is reffered to as primary culture.

9. S E C O N D A R Y C U LT U R E
O R C E L L L I N E
o The primary culture or the original tissue contains
many(heterogeneous )type of cells and also have variable growth
functions.
o Subculturing a fraction of cells from the primary culture yields a
more homogenous culture of cells. This culture is called a cell
line, which can be propagated, characterized and stored

10. D I P L O I D C E L L S T R A I N
o In the cell line culture, still there may be several cell lineages.
Among the various cell lineages, a Particular cell lineage with
specific properties is identified from the bulk of cells and then
cultured. This is then described as strain.
o In other words, all strain is derived from a single cell type with
specific stable properties like having marker chromosomes,
marker enzymes or antigens.

11. C O N T I N U O U S C E L L
L I N E
o All the above described culture is derived from a normal tissue that can
be sub-culture only for a limited number of times with the cells.
generally dividing for 20-100 times (ie, they flick limit) and the cells in
culture finally die out .This is called cellular Senescence and may be
connected to aging process. Thus culture from a normal tissue is finite.
o But if cells are obtained from a malignant tissue or if they a mutated,
then such cell lines are capable of undergoing infinite number of
divisions in culture ie, they are immortal" This is referred to as
continuous cell line.

12. R E F E R E N C E S

13. THANK YOU