Laura Ann Kelley is an experienced molecular and cell biologist currently working as an Associate Scientist at Eli Lilly & Company. She has over 10 years of experience in industry and academia employing techniques such as flow cytometry, high throughput screening assays, tissue/cell culture, molecular cloning, and more. Previously she has worked at Cedars-Sinai Medical Center, Amcyte Diabetes, and the University of California, San Diego conducting research in areas like antibody therapeutics, stem cells, and virology. She holds an M.S. in Biology from the University of Nevada, Las Vegas and has authored several publications and posters.
Dr. Talita Resende - Organoids as an invitro model for enteric diseasesJohn Blue
Organoids as an invitro model for enteric diseases - Dr. Talita Resende, from the 2018 Allen D. Leman Swine Conference, September 15-18, 2018, St. Paul, Minnesota, USA.
More presentations at http://www.swinecast.com/2018-leman-swine-conference-material
Next Generation Sequencing for Identification and Subtyping of Foodborne Pat...nist-spin
"Next Generation Sequencing for Identification and Subtyping of Foodborne Pathogens" presentation at the Standards for Pathogen Identification via NGS (SPIN) workshop hosted by National Institute for Standards and Technology October 2014 by Rebecca Lindsey, PhD from Enteric Diseases Laboratory Branch of the CDC.
Utilizing system biology resources to decipher a tritrophic disease complex presented at 2017 Annual meeting of Entomological Society of America at Denver, Colorado
Dr. Talita Resende - Organoids as an invitro model for enteric diseasesJohn Blue
Organoids as an invitro model for enteric diseases - Dr. Talita Resende, from the 2018 Allen D. Leman Swine Conference, September 15-18, 2018, St. Paul, Minnesota, USA.
More presentations at http://www.swinecast.com/2018-leman-swine-conference-material
Next Generation Sequencing for Identification and Subtyping of Foodborne Pat...nist-spin
"Next Generation Sequencing for Identification and Subtyping of Foodborne Pathogens" presentation at the Standards for Pathogen Identification via NGS (SPIN) workshop hosted by National Institute for Standards and Technology October 2014 by Rebecca Lindsey, PhD from Enteric Diseases Laboratory Branch of the CDC.
Utilizing system biology resources to decipher a tritrophic disease complex presented at 2017 Annual meeting of Entomological Society of America at Denver, Colorado
Exosomes are smallest extracellular vesicles of size 30 to 100 nm originated from late endosomes. These are released by broad array of cells including B‐ cells, cells, dendritic cells (DCs), T‐cells, epithelial cells,
platelets and many more.
Genes and Tissue Culture Assignment Presentation (Group 3)Lim Ke Wen
The culture of cells in two dimensions does not reproduce the histological characteristics of a tissue for informative or useful study. Growing cells as three-dimensional (3D) models more analogous to their existence in vivo may be more clinically relevant. Discuss the potential of using three dimensional cell cultures for anti-cancer drug screening.
A normal cell can be transformed into a cancerous cell. Discuss the therapeutic strategies that are employed to target the cellular transformation process for cancer prevention and treatment.
Lecture presented by Dr.Fatma Taha at BIOCHEM Cairo 2014 organized by Department of Medical Biochemistry and Molecular Biology, Cairo University. BIOCHEM Cairo 2014 is a Scribe event ( www.scribeofegypt.com)
The culture of cells in two dimensions does not reproduce the histological characteristics of a tissue for informative or useful study. Growing cells as three-dimensional (3D) models more analogous to their existence in vivo may be more clinically relevant. Discuss the potential of using three dimensional cell cultures for anti-cancer drug screening.
https://www.creative-bioarray.com/support/resazurin-cell-viability-assay.htm
Resazurin cell viability assay is a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability.
Oncolytic virus immunotherapy is a therapeutic approach to cancer treatment that utilizes native or genetically modified viruses that selectively replicate within tumor cells.
https://www.creative-biolabs.com/oncolytic-virus/
Not many people seem to know what a testimonial should
read like. I see poor testimonials all the time whenever
I do critiques for clients.
Do you know what a good testimonial looks like?
An effective testimonial should have the following elements in them:
Exosomes are smallest extracellular vesicles of size 30 to 100 nm originated from late endosomes. These are released by broad array of cells including B‐ cells, cells, dendritic cells (DCs), T‐cells, epithelial cells,
platelets and many more.
Genes and Tissue Culture Assignment Presentation (Group 3)Lim Ke Wen
The culture of cells in two dimensions does not reproduce the histological characteristics of a tissue for informative or useful study. Growing cells as three-dimensional (3D) models more analogous to their existence in vivo may be more clinically relevant. Discuss the potential of using three dimensional cell cultures for anti-cancer drug screening.
A normal cell can be transformed into a cancerous cell. Discuss the therapeutic strategies that are employed to target the cellular transformation process for cancer prevention and treatment.
Lecture presented by Dr.Fatma Taha at BIOCHEM Cairo 2014 organized by Department of Medical Biochemistry and Molecular Biology, Cairo University. BIOCHEM Cairo 2014 is a Scribe event ( www.scribeofegypt.com)
The culture of cells in two dimensions does not reproduce the histological characteristics of a tissue for informative or useful study. Growing cells as three-dimensional (3D) models more analogous to their existence in vivo may be more clinically relevant. Discuss the potential of using three dimensional cell cultures for anti-cancer drug screening.
https://www.creative-bioarray.com/support/resazurin-cell-viability-assay.htm
Resazurin cell viability assay is a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability.
Oncolytic virus immunotherapy is a therapeutic approach to cancer treatment that utilizes native or genetically modified viruses that selectively replicate within tumor cells.
https://www.creative-biolabs.com/oncolytic-virus/
Not many people seem to know what a testimonial should
read like. I see poor testimonials all the time whenever
I do critiques for clients.
Do you know what a good testimonial looks like?
An effective testimonial should have the following elements in them:
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Current CV .
My objective is to obtain a rewarding and challenging research scientist position where my background and experience will contribute to the success of a growing company or research center.
Currently, I am a Senior Associate Scientist at Amgen Inc. and certified Molecular Biologist with the American Society of Clinical Pathology MB (ASCP). I have more than 10 years of experience in the biotechnology/ pharmaceutical industry. I am highly proficient in various lab techniques, technologies, and automation. I demonstrated consistent success in the execution of assay development and method validation activities supporting clinical stage programs within GCP and GLP regulated environments. I possess extensive experience in optimization and validation of drug potency assays (ELISA and cell based assays), protein purification and characterization, and DNA/RNA extraction and quantitation. I am a subject matter expertise in the areas of human and rodent cell lines propagation and tissue dis-aggregation. I have proven operational capabilities in the establishment of standard operating procedures to ensure our laboratory meets regulatory and business requirements.
I am a self-motivated professional who works effectively as an individual contributor or within a team matrix. As a quick learner, I can efficiently deliver results, easily adapt to changing environment and provide fresh ideas. My strengths include statistical analysis/guidance, report writing, and communication.
Thank you in advance for your consideration. Please feel free to call me at (805-990-6258), or by e-mail at (mahawally46@gmail.com) if you have questions or would like a list of references.
Sincerely,
Maha Rizk
Molecular Biologist Academic CV for Industry or Private Sector Consideration Sirie Godshalk
Molecular Biologist with over thirteen years of hands-on research experience, impactful writer and presenter, dynamic leader and enthusiastic team player with an eye for great ideas and a passion to move science in new directions seeks challenging opportunities beyond the bench.
1. Laura Ann Kelley
San Diego,CA 92124
lakelley310@gmail.com
Profile
Experienced Molecular and Cell Biologist with 10+ years of experience in industry and academia. Currently
working as an Associate Scientist for Eli Lilly & Company where I employ a variety of molecular and cellular
techniques in drug discovery and development.
Proficientin flow cytometry sample preparation, acquisition (LSRII,LSRFortessa, FACSCanto,
FACSAria, CyTof) and analyses (FlowJo,FACSDiva)
96 and 384 well High Throughput Screening Assay development
Tissue/cell culture of suspension and adherent cell lines and primary mammalian cells
In vitro assays (Bioplex 200, MSD, Cell Proliferation,Cell Death, Necrosis & Apoptosis, Reporter
Gene Assays, Functional Bioassays)
Molecular cloning techniques (DNA/RNA extraction and quantification, primer design, vector
design, ligation restriction digestion, PCR, DNA sequencing, bacterial transformation, protein
transfection),
General molecular, protein and biochemistry techniques (chemical and radio isotopic proliferation
and cytotoxicity assays,ELISA, Western blotting, protein purification techniques by metal affinity
chromatography, SDS-PAGE /Agarose gel electrophoresis)
Human and murine immune cell isolation (MACS) and characterization from pBMC and whole blood
Experience in virology techniques for transduction and working with live MCMV forpre-clinical
vaccinemodels
Additional training (Animal care and use program, Mouse/Rat handling techniques, Blood-borne
pathogen training, Radiation safety training )
Professional experience
Eli LillyandCompany,San Diego, CA, Associate Consultant Biologist (August 2007-present)
Responsible forprocessing and analysis of large multicolor flow cytometry panels involving surface
and intracellular staining of fresh and cultured human PBMCs, mouse splenocytes and lymph nodes
Optimize human mixed lymphocytereactions for bio-assay of antibodies
PerformHigh Throughput flow cytometry binding assays
Performin vitro cell based assays (ELISA,Luminex) for cytokines,chemokines, and
immunoglobulin from cells and condition medium
Train colleagues on FACSDiva software, proper instrument use, and education in multicolor flow
cytometry acquisition and analysis.
Optimize single cell PCR, high throughput cloning and expression protocols forfull length
antibodies in mammalian cells and demonstrate and troubleshoot the applicability and diversity of
scPCR across a wide variety of targets for pharmaceutical drug development
Support team projects by assisting with desired molecular biology work.
2. Cedars-SinaiMedical Center,LosAngeles, CA, ProjectResearch Associate II (June2006-August 2007)
Tissue culture, processing and analysis of tumor celllines used in the development of antibody
therapeutics forpre-clinical drug discovery in prostate cancer research
Transient transfection of suspension and adherent mammalian cell lines and gene and expression
of secreted proteins to identify prostate cancerprotein markers as prognostic factors
Western Blot analyses
Cell based proliferation and cytotoxicity assays
Cloning of targeted genes into expression vectors
Amcyte DiabetesInc.,Santa Monica, CA, ProjectResearch Associate II (October2004-June 2006)
Progenitor stem cell culturing, proliferation and maintenance (adult human and neonatal porcine)
Sample preparation for DNA, RNA (RT-PCR) and immunohistochemistry assays and
immunofluorecent staining
Sample preparation for FACS sorting
UniversityofCalifornia,SanDiego,ResearchAssociate/Asst Lab Manager (April 2003-October 2004)
Infectionand propagation of MCMV in culture
Viral preparation and titers transfection and gene expression of viral antigenic proteins
Plasmid DNA preparation and dosing for in vivovaccination studies
Education
UniversityofNevada,Las Vegas
M.S., Biology,May 2002.
B.S., Biology,May 1998
Publications/Posters
Morello CS, KelleyLA,Munks MW,Hill AB, Spector DH. Cytomegalovirus genes encoding homologs of
human cytomegalovirusUL54 (DNA polymerase) and UL105 (helicase) elicits strong CD8 T-cellresponses
and is protective against systemic challengeJ Virol. 2007 Jul;81(14):7766-75. Epub 2007 May 16.
Monica Florio, ErikPong, Laura Kelley,Wen-GhihTsang and Aleksandar Francki. Growth Factor-mediated
Activationof Akt1 in Adult Primary Human Pancreatic Cells Enhances the Survivaland Proliferationof
Endocrine Lineage Cells. AmCyte Inc., Santa Monica, California 90404, USA.
Keystone Symposia Poster. Pancreatic Islets: From Development to Transplantation, February 23-28,
2006, Sagebrush Inn and Conference Center, Taos, New Mexico
3. Morello CS, YeM, Hung S, KelleyLA,Spector DH. Systemic priming-boosting immunization with a
trivalent plasmid DNA and inactivated murine cytomegalovirus (MCMV) vaccineprovides long-term
protection against viral replication following systemic or mucosal MCMV challenge.
J Virol. 2005 Jan; 79(1):159-75.
Riddle, BR, SJ Mantooth, L Kelley,and T Lawlor.Population structure and mountain-top phylogeography of
the Tamiasumbrinus species complex in western North: expansion, migration, and diversificationin
response to changing climates. American Society of Mammologists, Lubbock,TX;2003.
Laura A. Kelleyand Brett R. Riddle, Is Palmer’s chipmunk (T. palmeri) a distinct evolutionarily significant
unit (ESU)withinthe Uinta chipmunk (T. umbrinus) species group? Department of Biological Sciences,
University of Nevada, Las Vegas, Las Vegas, Nevada 89154-4004. Annual meeting of the American Society
of Mammalogists, Missoula, MT; 2001.