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SANDRA ANDERSON LASALLE
5600 Millwheel Way
Henrico, VA 23228
Email: sandy.belsky@yahoo.com
Mobile: (757) 822-9181
OBJECTIVE: Seeking a position as scientist using past education, scientific and/or clinical
knowledge.
CAREER SUMMARY:
 Over 20 years of experience in clinical
and basic science research
 Substantial experience using
computerized information systems,
Outlook, Word, Excel, PowerPoint,
teleconferencing
 Advanced knowledge of Microbiology
techniques
 Over 10 years’ experience in
laboratory management
 Experience in product development
and quality assurance in
pharmaceutical industry to include
Merck and Pfizer.
 Experience in quality assurance and
data validation in basic science and all
aspects of clinical industry, medical
schools, and private companies.
 Therapy areas include research and
development in oncology, diabetes
and female reproduction
EDUCATION:
1987-1988: Master’s Program, - Molecular Genetics, 10 credits
Rutgers University, New Brunswick, NJ
1986: Bachelor of Science - Microbiology – Dean’s List
Rutgers University, New Brunswick, NJ
1982: Associate of Applied Science - Marketing
Middlesex County College, Edison, NJ
EXPERIENCE:
March 7, 2016 – June 6, 2016 Henrico, Virginia
Biology Tutor, Henrico Public Schools
 Provide science education for 100 high school students to include science activities, lesson
plans and one-on-one assistance/teaching with students.
 Interact with teachers, staff, students to maintain order, accomplishment goals.
March 2014-June 2015 Richmond, Virginia
Contract Product Design Scientist, Pfizer
 Research and development of consumer health products: to improve or build upon current
uses. Products include Caltrate, Centrum, energy drinks and other dietary products.
 Full design and product development: tablet compression, stability trials, physical tests,
GMP work with strict adherence to standard operating procedures.
 Developed prototype gummy vitamins to incorporate consumer studies for gummy vitamins.
Prototype energy protein shakes. Interacted with Pfizer marketing professionals and
designed simplified method for an evening of hands-on gummy formation by the business
group. Detailed records for over 29 trials, various methods.
June 2009-June 2013 Norfolk, Virginia
ResearchLab Manager-Jones Institute for Reproductive Medicine
Eastern Virginia Medical School
 Researcher in embryo implantation and fertilization processes to ultimately increase
viability of intro fertilization with patients.
 3D Culture: grow up to 4 cell lines in a plasma-based construct, paraffin-embed for IHC
analysis of invasion and laser confocal microscope analysis for viability, depth of invasion.
 Develop proliferation and adhesion assay to study a protein’s role in implantation. IHC of
human and mouse tissues. Silence MfgE8 in human mammalian cell line and assess via
PCR, Westerns.
 Performed q PCR to assess 15 genes of 6 cell culture lines, primary cells and mouse embryo
RNA, human endometrial tissue.
 Lab manager: manage the budget, maintain lab and train new MD fellows, visiting scholars.
August 2007- July 2008 Norfolk, Virginia
ResearchAssociate II- Frank Reidy Research Center for Bioelectrics
Old Dominion University
 Preclinical research in oncology to study Melanoma mechanisms and necrosis/apoptosis
pathways for alternative therapies.
 Investigated Melanoma and blood flow of tumors in mice using trans-illumination and
ultrasound using these poration techniques in therapy.
 Collaborated with a medical device company to use nanoporation to create apoptosis of
adipose tissue in human and non-human tissue ultimately for cosmetic use.
 Trained on the FACS Aria Cell Sorter with BD Bioscience, San Jose, CA July 16-20, 2007
 Established cell sorting and flow cytometry capabilities in order to become a reference
laboratory.
August 2005 – August 2007 Norfolk, Virginia
ResearchAssociate II, Eastern Virginia Medical School – Physiology/Ctr for Bioelectrics
 Investigated use of nanosecond pulsed electric fields (nsPEF) on Melanoma tumors in mice
as alternative standard therapies.
 Developed immunohistochemical labeling of antibodies to assess nsPEF and irradiation in
cells using the laser scanning confocal microscope.
 Isolated cell clones, performed clonogenic analysis, caspase activation via flow
 Trained students in cell culture, flow cytometry, and tissue preparation
September 2003 – September 2004 Virginia Beach, Virginia
ResearchMicrobiologist, LifeNet
 Research in bio-implant technologies and cellular therapies to include new medical
applications for allografts and tissue engineering for possible FDA approvals.
 Sole project leader in quality compliance and validation for donor human tendon and bone
allografts for ultimate transplantation.
 Performed good laboratory practice and developed protocols and SOPs.
 Completed the validation and results presented at American Association of Tissue Banking
(Chicago, 8/04) and to the FDA (9/1/04).
July 2002 – September 2003 Norfolk, Virginia
ResearchAssociate, Strelitz Diabetes Institute
 Performed research in transgenic mouse lines to investigate role of genes in Diabetes I, II.
 Investigated diabetic neuropathy by analyzing human skin biopsies with double antibody
labeling to study nerve densities and dendritic length. Quantitated via laser confocal
microscope.
 Funded $150,000 grant by Johnson & Johnson based on neuropathy data.
1988 – 1991 Rahway, New Jersey
Molecular Biologist, Molecular Pharmacology Department
Merck Sharp and Dohme Research Labs
 Studied human, rat substance P receptor and develop drugs to modulate the pain response.
 Subcloned human substance P receptor into expression vectors.
 Screened lambda, bacterial libraries with synthesized DNA probes. Characterized PCR-
generated fragments by sequencing, identified splicing variants.
 Studied the minimum inhibitory concentration of an antifungal drug on Saccharomyces
cerevisae and assisted in design of antifungal drug screen.
 Initiated and maintained cell culture lines for a group of 15 amidst yeast lab.
1986 – 1988 New Brunswick, New Jersey
ResearchSpecialist III, Robert Wood Johnson Medical School,
 Isolated rat aorta RNA and mRNA for a phage cDNA library for studies of hypoxia-induced
hypertension.
 Screened and sequenced rat elastin clones, studied mRNA levels of hypoxic rats
 Poster presentation: “Elastin synthesis in the hypertensive pulmonary artery of the rat.”
Woods Hole, Massachusetts, 1987.
 Presented poster at NJ Thoracic Society Annual Meeting, April 1987.
RECENT PUBLICATIONS (selected)
Schmitz, C, Yu, L, Bocca,S, Anderson S,Cunha-Filho, BS, Rhavi, B., and Oehninger, S.
A role for the endometrial epithelial protein MFG-E8 and its receptor integrin αvβ3 in
human implantation: results of an in vitro trophoblast attachment study using established
human cell lines. Fertility and Sterility, accepted Dec 2013.
Wang H, Bocca S, Anderson S,Yu L, Rhavi BS, Horcajadas J,Oehninger S. Sex Steroids Regulate
Epithelial-Stromal Cell Cross Talk and Trophoblast Attachment Invasion in a Three-Dimensional Human
Endometrial Culture System. Tissue Eng Part C Methods. Epub 2013 Feb 19.
Lu Y, Bocca S, Anderson S,Wang H, Manhua C, Beydoun H, Oehninger S. Modulation of the Expression
of the Transcription Factors T-Bet and GATA-3 in Immortalized Human Endometrial Stromal Cells
(HESCs) by Sex Steroid Hormones and cAMP. Reprod Sci. 2013 Jun; 20(6):699-709. doi:
10.1177/1933719112466297. Epub 2013 Jan 10.
Riggs RM, Bocca S, Anderson S,Franchi A, Rhavi BS, Oehninger S. Epithelial cell protein milk fat
globule-epidermal growth factor 8 and human chorionic gonadotropin regulate stromal cell apoptosis in the
human endometrium. Fertil Steril. 2012 Dec; 98(6):1549-56.e3. doi: 10.1016/j... Ferti Steril.2012.07.1127.
Epub 2012 Aug 24.
Wang H, Pilla F, Anderson S,Martínez-Escribano S, Herrer I,Moreno-Moya JM, Musti S, Bocca S,
Oehninger S, Horcajadas JA. A novel model of human implantation: 3D endometrium-like culture system to
study attachment of human trophoblast (Jar) cell spheroids. Mol Hum Reprod. 2012 Jan; 18(1):33-43. Epub
2011 Oct 11.
Franchi A, Bocca S, Anderson S,Riggs R, Oehninger S. Expression of Milk Fat Globule EGF-Factor
*(MFG-E8) mRNA and protein in the human endometrium and its regulation by prolactin. Mol Hum Reprod.
2011 17: 360-371. Doi:10.1093/molehr/gaqq102.

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CV LaSalleSandra

  • 1. SANDRA ANDERSON LASALLE 5600 Millwheel Way Henrico, VA 23228 Email: sandy.belsky@yahoo.com Mobile: (757) 822-9181 OBJECTIVE: Seeking a position as scientist using past education, scientific and/or clinical knowledge. CAREER SUMMARY:  Over 20 years of experience in clinical and basic science research  Substantial experience using computerized information systems, Outlook, Word, Excel, PowerPoint, teleconferencing  Advanced knowledge of Microbiology techniques  Over 10 years’ experience in laboratory management  Experience in product development and quality assurance in pharmaceutical industry to include Merck and Pfizer.  Experience in quality assurance and data validation in basic science and all aspects of clinical industry, medical schools, and private companies.  Therapy areas include research and development in oncology, diabetes and female reproduction EDUCATION: 1987-1988: Master’s Program, - Molecular Genetics, 10 credits Rutgers University, New Brunswick, NJ 1986: Bachelor of Science - Microbiology – Dean’s List Rutgers University, New Brunswick, NJ 1982: Associate of Applied Science - Marketing Middlesex County College, Edison, NJ EXPERIENCE: March 7, 2016 – June 6, 2016 Henrico, Virginia Biology Tutor, Henrico Public Schools  Provide science education for 100 high school students to include science activities, lesson plans and one-on-one assistance/teaching with students.  Interact with teachers, staff, students to maintain order, accomplishment goals. March 2014-June 2015 Richmond, Virginia Contract Product Design Scientist, Pfizer  Research and development of consumer health products: to improve or build upon current uses. Products include Caltrate, Centrum, energy drinks and other dietary products.  Full design and product development: tablet compression, stability trials, physical tests, GMP work with strict adherence to standard operating procedures.  Developed prototype gummy vitamins to incorporate consumer studies for gummy vitamins. Prototype energy protein shakes. Interacted with Pfizer marketing professionals and designed simplified method for an evening of hands-on gummy formation by the business group. Detailed records for over 29 trials, various methods.
  • 2. June 2009-June 2013 Norfolk, Virginia ResearchLab Manager-Jones Institute for Reproductive Medicine Eastern Virginia Medical School  Researcher in embryo implantation and fertilization processes to ultimately increase viability of intro fertilization with patients.  3D Culture: grow up to 4 cell lines in a plasma-based construct, paraffin-embed for IHC analysis of invasion and laser confocal microscope analysis for viability, depth of invasion.  Develop proliferation and adhesion assay to study a protein’s role in implantation. IHC of human and mouse tissues. Silence MfgE8 in human mammalian cell line and assess via PCR, Westerns.  Performed q PCR to assess 15 genes of 6 cell culture lines, primary cells and mouse embryo RNA, human endometrial tissue.  Lab manager: manage the budget, maintain lab and train new MD fellows, visiting scholars. August 2007- July 2008 Norfolk, Virginia ResearchAssociate II- Frank Reidy Research Center for Bioelectrics Old Dominion University  Preclinical research in oncology to study Melanoma mechanisms and necrosis/apoptosis pathways for alternative therapies.  Investigated Melanoma and blood flow of tumors in mice using trans-illumination and ultrasound using these poration techniques in therapy.  Collaborated with a medical device company to use nanoporation to create apoptosis of adipose tissue in human and non-human tissue ultimately for cosmetic use.  Trained on the FACS Aria Cell Sorter with BD Bioscience, San Jose, CA July 16-20, 2007  Established cell sorting and flow cytometry capabilities in order to become a reference laboratory. August 2005 – August 2007 Norfolk, Virginia ResearchAssociate II, Eastern Virginia Medical School – Physiology/Ctr for Bioelectrics  Investigated use of nanosecond pulsed electric fields (nsPEF) on Melanoma tumors in mice as alternative standard therapies.  Developed immunohistochemical labeling of antibodies to assess nsPEF and irradiation in cells using the laser scanning confocal microscope.  Isolated cell clones, performed clonogenic analysis, caspase activation via flow  Trained students in cell culture, flow cytometry, and tissue preparation September 2003 – September 2004 Virginia Beach, Virginia ResearchMicrobiologist, LifeNet  Research in bio-implant technologies and cellular therapies to include new medical applications for allografts and tissue engineering for possible FDA approvals.  Sole project leader in quality compliance and validation for donor human tendon and bone allografts for ultimate transplantation.  Performed good laboratory practice and developed protocols and SOPs.  Completed the validation and results presented at American Association of Tissue Banking (Chicago, 8/04) and to the FDA (9/1/04). July 2002 – September 2003 Norfolk, Virginia ResearchAssociate, Strelitz Diabetes Institute  Performed research in transgenic mouse lines to investigate role of genes in Diabetes I, II.  Investigated diabetic neuropathy by analyzing human skin biopsies with double antibody labeling to study nerve densities and dendritic length. Quantitated via laser confocal microscope.
  • 3.  Funded $150,000 grant by Johnson & Johnson based on neuropathy data. 1988 – 1991 Rahway, New Jersey Molecular Biologist, Molecular Pharmacology Department Merck Sharp and Dohme Research Labs  Studied human, rat substance P receptor and develop drugs to modulate the pain response.  Subcloned human substance P receptor into expression vectors.  Screened lambda, bacterial libraries with synthesized DNA probes. Characterized PCR- generated fragments by sequencing, identified splicing variants.  Studied the minimum inhibitory concentration of an antifungal drug on Saccharomyces cerevisae and assisted in design of antifungal drug screen.  Initiated and maintained cell culture lines for a group of 15 amidst yeast lab. 1986 – 1988 New Brunswick, New Jersey ResearchSpecialist III, Robert Wood Johnson Medical School,  Isolated rat aorta RNA and mRNA for a phage cDNA library for studies of hypoxia-induced hypertension.  Screened and sequenced rat elastin clones, studied mRNA levels of hypoxic rats  Poster presentation: “Elastin synthesis in the hypertensive pulmonary artery of the rat.” Woods Hole, Massachusetts, 1987.  Presented poster at NJ Thoracic Society Annual Meeting, April 1987. RECENT PUBLICATIONS (selected) Schmitz, C, Yu, L, Bocca,S, Anderson S,Cunha-Filho, BS, Rhavi, B., and Oehninger, S. A role for the endometrial epithelial protein MFG-E8 and its receptor integrin αvβ3 in human implantation: results of an in vitro trophoblast attachment study using established human cell lines. Fertility and Sterility, accepted Dec 2013. Wang H, Bocca S, Anderson S,Yu L, Rhavi BS, Horcajadas J,Oehninger S. Sex Steroids Regulate Epithelial-Stromal Cell Cross Talk and Trophoblast Attachment Invasion in a Three-Dimensional Human Endometrial Culture System. Tissue Eng Part C Methods. Epub 2013 Feb 19. Lu Y, Bocca S, Anderson S,Wang H, Manhua C, Beydoun H, Oehninger S. Modulation of the Expression of the Transcription Factors T-Bet and GATA-3 in Immortalized Human Endometrial Stromal Cells (HESCs) by Sex Steroid Hormones and cAMP. Reprod Sci. 2013 Jun; 20(6):699-709. doi: 10.1177/1933719112466297. Epub 2013 Jan 10. Riggs RM, Bocca S, Anderson S,Franchi A, Rhavi BS, Oehninger S. Epithelial cell protein milk fat globule-epidermal growth factor 8 and human chorionic gonadotropin regulate stromal cell apoptosis in the human endometrium. Fertil Steril. 2012 Dec; 98(6):1549-56.e3. doi: 10.1016/j... Ferti Steril.2012.07.1127. Epub 2012 Aug 24. Wang H, Pilla F, Anderson S,Martínez-Escribano S, Herrer I,Moreno-Moya JM, Musti S, Bocca S, Oehninger S, Horcajadas JA. A novel model of human implantation: 3D endometrium-like culture system to study attachment of human trophoblast (Jar) cell spheroids. Mol Hum Reprod. 2012 Jan; 18(1):33-43. Epub 2011 Oct 11. Franchi A, Bocca S, Anderson S,Riggs R, Oehninger S. Expression of Milk Fat Globule EGF-Factor *(MFG-E8) mRNA and protein in the human endometrium and its regulation by prolactin. Mol Hum Reprod. 2011 17: 360-371. Doi:10.1093/molehr/gaqq102.