1. Resume
VASUDEVAN AYYAPPAN
Phone: 848 228 9092
Email: vayyappan@desu.edu
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SUMMARY
Broadly trained biologist with expertise in mammalian, microbial and plant research
Research Strength: 10+ years working experience in Molecular Biological Techniques, and 5+
experience in Next Generation Sequencing, Genome Editing and Pathway Analysis
Excellent knowledge in design, optimization and implementation of complex experiments
Proven ability to effectively collaborate with multiple research teams, quickly scale-up lab
processes and meet tight deadlines
Actively seeking new job opportunities
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EDUCATION
Bharathidasan University, India
Ph.D. in Biotechnology 2006
Madurai Kamaraj University, India
M.S. in Microbiology 2000
Madurai Kamaraj University, India
B.S. in Botany 1998
SCIENTIFIC SKILLS & TECHNIQUES
Molecular Biology: CRISPR/Cas9 Genome Editing, Gene Knockout models in animals, RNA
and Plasmid/genomic DNA isolation; PCR; RT-PCR; qRT - PCR, RAPD; RFLP; PFGE; In Vitro
Transcription, Southern and Northern blotting; Cloning and cDNA Library Construction;
Protoplast Culture, Yeast2Hybrid
Biochemistry: ELISA; SDS-PAGE and Western blotting; HPLC; GC; GC Spectroscopy (UV
and Visible); Expression of GFP in transgenic Plants; GUS assay: Determination of ethylene;
Assay of ACC oxidase; Herbicide resistance analysis in Cucumber and Sugarcane with
glyphosate and Basta; MTT & MTS assay; Transfection

2. Next Generation Sequencing: Sample and Illumina library preparation for Chromatin
Immunoprecipitation-Seq (ChIP-Seq), RNA-Seq and Methyl-Seq.
Bioinformatics and large-scale sequence analysis: CLC Bio work bench
Mammalian Cell Culture: Multiple myeloma, Pancreatic and Breast cancer cell lines
Microbial Cell Culture: E.coli and fungal cell cultures, and Cell viability assay
Plant Tissue Culture and Transformation: In Tobacco, Cucumber, Common Bean, Rice and
Sugarcane; Agro-infiltration, Post-transcriptional gene silencing (PTGS), Production of
transgenic plants for viral coat proteins
Gene Knockout: CRISPR/Cas9 genome editing in Tobacco and Common bean
Imaging assays: GFP (Green Fluorescent Protein) analysis using confocal microscopy, GUS
reporter assay in transformed plants
Enzymatic Assays: Genes involved in growth and development of animals and plants
Pathway analysis: Identification of genes involved in biotic and abiotic stresses
Laboratory Management: Guidance to undergraduate and graduate students, ordering and
maintaining chemicals and laboratory equipment
PROFESSIONAL EXPERIENCE
Research Scientist (Delaware State University) 2014- Present
Title: Developing epigenomic and transcriptomic tools in bioenergy crop - switchgrass
 Developed chromatin immunoprecipitation sequencing (ChIP-Seq) protocol in
switchgrass for the first time
 Developed transcriptomic data from different geographical locations of switchgrass
 Identified biomass production and yield associated key enzymes in monolignol, C4-
photosynthesis, and photorespiratory pathways and genes belonged to various abiotic
stresses and disease resistance
Post-Doctoral Research Associate (Delaware State University) 2010 - 2014
Title: Molecular and epigenetic interaction in common bean with reference to fungal rust
infection
 Assessed selected epigenetic modifications at the whole genome level by using a
combination of methyl-C profiling, chromatin immunoprecipitation sequencing (ChIP-
Seq), and transcriptional profiling in response to fungal rust
 Identified 10 key defense responsive genes and 25 transcription factors in bean-rust
interaction
 This is the first report of an integrated analysis of histone modifications and gene
expression involved in the bean-rust interaction

3. Post-Doctoral Researcher (Rutgers University) 2009 – 2010
Title: Mechanism of cell killing by the aminoglycoside antibiotic streptomycin in Escherichia
coli
 Worked on an interdisciplinary NIH project “Mechanism of cell-killing by the
aminoglycoside antibiotic streptomycin in Escherichia coli” specifically investigating an
unexpected mutator phenotype observed in cells bearing mutant tRNA genes
 Conducted research on translational stress-induced mutagenesis (TSM) by elevated
mistranslation (through any means) that induces a mutator response
 Studied the functional recA gene for the expression of mutator phenotype that mediated
by mistranslation corruption of DNA polymerase III. Determined the effect of
aminoglycoside antibiotic streptomycin, which is known to promote mistranslation and
which ultimately completely blocks protein synthesis, also induces the TSM phenotype
under certain condition
Post-Doctoral Researcher (Seoul National University, Seoul, South Korea) 2008-2009
Title: Molecular mechanism of trans-dihydronarciclasine on multiple myeloma
 Conducted NRF sponsored novel research on “Isocarbostryl derivative, Trans-
dihydronarciclasine HYU-01, induces apoptosis and diminishes proliferation of human
multiple myeloma U266 cells”
 Investigated the effect of a novel synthetic compound trans-dihydronarciclasine (HYU-
01) on the induction of apoptosis in MM cells via the activation of apoptosis related
proteins by the inhibition of cell signaling pathways
 Studied the molecular mechanisms regulated by Trans-dihydronarciclasine and their
analogs during induction of apoptosis
Post-Doctoral Researcher (Pusan National University, Busan, South Korea) 2007 – 2008
Title: Characterization of Thioredoxin (TRXm) over expression in Arabidopsis thaliana
 The functional characterization of TRX over expression lines (Tg8 and Tg10) in
Arabidopsis under various abiotic stresses have been studied
 Gene involved in first committed step of de novo fatty acid biosynthesis namely ACCase
(Acetyl-CoA carboxylase) have been monitored and it was shown that this gene highly
expressed in TRX over expression lines compared with wild type and vector only plants
Research Assistant Professor (Pai Chai University, Daejeon, South Korea) 2005-2007
Title: Research in Plant Virology and Functional Genomics
 Studied the suppression of RNA silencing mechanism to overcome non-host type
resistance in Nicotiana benthamiana by synergistic interaction between Soybean mosaic
virus and Tobacco etch virus
 Developed transgenic protocol to produce tobacco plants that confers Turnip Crinkle
Virus-Coat Protein for viral resistance
 Cloned Porcine CircoVirus – Coat Protein (PCV-CP) in Tobacco Mosaic Virus (TMV)
vector for edible vaccine using Cucumber as a model system

4. SCIENTIFIC PUBLICATIONS
1. Ayyappan, V, Kalavacharla V, Thimmapuram, J, Bhide KP, Sripathi, VR, Smolinski, TG,
Manoharan M, Thurston Y, Todd A. and Kingham B. (2015). Genome Wide Profiling of Histone
Modifications (H3K9me2 and H4K12ac) and Gene Expression in Rust (Uromyces appendiculatus)
infected Common Bean (Phaseolus vulgaris L.). PLoS ONE. 10(7): e0132176
2. Humayun Z. and Vasudevan A (2013). Potential roles for DNA replication and repair
functions in cell killing by streptomycin. Mut Res. 749(1-2): 87-91
3. Udayakumar R, Kasthurirengan S, Vasudevan A, Mariashibu TS, Rayan JJS, Choi CW,
Ganapathi A. and Kim SC. (2010). Antioxidant Effect of Dietary Supplement Withania
somnifera L. Reduce Blood Glucose Levels in Alloxan-Induced Diabetic Rats. Plant Foods for
Human Nutrition. 65(2): 91-98
*4. Selvaraj N, Kasthurirengan S, Vasudevan A, Choi CW. and Ganapathi A. (2010). Evaluation
of green fluorescent protein as a reporter gene and phosphinothricin as the selective agent for
achieving a higher recovery of transformants in cucumber (Cucumis sativus L. cv. Poinsett76)
via Agrobacterium tumefaciens. In Vitro Cellular and Developmental Biology –Plant. 46 (4):
329-337
5. Park J, Vasudevan A, Bae EK, Lee C, Kim BS, Kim BK, Lee YY. Ahn KS. and Yoon SS.
(2008). Curcumin in combination with bortezomib synergistically induced apoptosis in human
multiple myeloma U266 cells. Molecular Oncology. 2: 317-326
6. Vasudevan A, Oh TK, Choi BK, Lee HJ, Ji J, Kim JH, Ganapathi A. and Choi CW. (2008).
Characterization of resistance mechanism in transgenic Nicotiana benthamiana containing
Turnip crinkle virus coat protein. Plant Cell Reports. 27: 1731-1740
7. Vasudevan A, Selvaraj N, Ganapathi A, Ramesh Anbazhagan V, Kasthuri Rengan S. and
Manickavasagam M. (2008). Leucine and Spermidine enhanced in vitro shoot multiplication in
cucumber (Cucumis sativus L.). In Vitro Cellular and Developmental Biology - Plant. 44 (4):
300-306
8. Vasudevan A, Selvaraj N, Ganapathi A, Ramesh Anbazhagan V, Kasthruri Rengen S. and
Manickavasagam M. (2007). Direct plant regeneration from cucumber embryonal axis. Biologia
Plantarum. 51(3): 521-524
**9. Selvaraj N, Vasudevan A, Manickavasagam M, Kasthurirengan S. and Ganapathi A.
(2007). High frequency shoot regeneration from cotyledon explants of cucumber via
organogenesis. Sci. Hortic. 112(1): 2-8
10. Vasudevan A, Selvaraj N, Ganapathi A. and Choi CW (2007). Agrobacterium-mediated
genetic transformation in cucumber (Cucumis sativus L.). American Journal of Biochemistry and
Biotechnology. 3(1): 24-32

5. 11. Park JS, Choi BK, Lakshmi SV, Vasudevan A, Chong CK, Lee KS, Kim SC. and Choi CW.
(2007). Immunodetection of Canine Parvovirus (CPV) in clinical samples by polyclonal antisera
against CPV-VP2 protein expressed in Escherichia coli as an antigen. J. Virol. Methods. 146:
181-187
12. Selvaraj N, Vasudevan A, Manickavasagam M. and Ganapathi A. (2006). In vitro
organogenesis and plantlet formation in cucumber. Biologia Plantarum. 50(1): 123-126
13. Vasudevan A. Ganapathi A. Selvaraj N, Choi CW. and Manickavasagam M. (2006).
Changes in L- Isoaspartyl Methyltransferase, storage components and anti –oxidants enzymes
during accelerated ageing in Cucumber (Cucumis sativus L.) seeds. Journal of Plant Sciences.
1(3): 228-239
14. Vasudevan A, Selvaraj N, Ganapathi A, Ramesh Anbazhagan V. and Choi CW. (2006).
Effect of ethylene inhibitors on In vitro shoot multiplication and their impact on ethylene
production in Cucumber (Cucumis sativus L.). Res J Bot. 1(3): 110-117
15. Choi BK, Park JS, Ahn HJ, Yum HJ, Vasudevan A, Lakshmi SV, Kim JS, Kim SC. and
Choi CW. (2006). Molecular Characterization of Soybean Mosaic 5 Virus NIa protein and its
processing event in bacterial expression. American Journal of Biochemistry and Biotechnology.
2(4): 148-153
16. Sureshkumar P, Selvaraj N, Ganapathi A, Kasthurirengan S, Vasudevan A. and Ramesh
Anbazhagan, V. (2005). Assessment of factors influencing Agrobacterium mediated
transformation in Cucumber (Cucumis sativus L.). J Plant Biotechnol. 7(4):225-231
17. Vasudevan A, Selvaraj N, Ganapathi A, Kasthuri Rengan S, Ramesh Anbazhagan V. and
Manickavasagam M. (2004). Glutamine: An ideal nitrogen source for enhanced shoot
multiplication in cucumber (Cucumis sativus L.). Biologia Plantarum. 48(1): 125-128
18. Manickavasagam M, Ganapathi A, Ramesh Anbazhagan V, Sudhakar B, Selvaraj N,
Vasudevan A. and Kasthuri Rengen S. (2004). Agrobacterium – mediated genetic
transformation and development of herbicide resistant sugarcane (Saccharum species hybrids)
using axially bud explants. Plant Cell Rep. 23:134-143
19. Muruganantham M, Ganapathi A, Selvaraj N, Prem Anand R, Vasudevan A. and
Vengadesan G. (2002). Role of Adenine sulphate and L-Glutamine on multiple shoot induction
from melon (Cucumis melo L.) cotyledon. Cucurbit Genetics Cooperative Report. 25: 24-27
20. Vasudevan A, Ganapathi A, Selvaraj N, Mururganantham M. and Vengadesan G. (2002).
Agrobacterium-mediated transformation in cucumber (Cucumis sativus L.). Cucurbit Genetics
Cooperative Report. 25: 14-16
21. Vasudevan A, Ganapathi A, Selvaraj N. and Vengadesan G. (2002). Factors influencing
GUS expression in cucumber (Cucumis sativus L.). Indian Journal of Biotech. 1: 344-349

6. 22. Vasudevan A, Selvaraj N, Suresh Kumar S. and Ganapathi A. (2001). Multiple shoot
induction from shoot tip explants of cucumber (Cucumis sativus L.). Cucurbit Genetics
Cooperative Report. 24: 8-12
*Paper appeared in Journal Highlights
** Paper appeared in the list of Top 25 articles in Science Direct in January – March, 2007